Detailed information of TA system TA100114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 4264591..4265119
• Replicon: chromosome
• Accession: NZ_CP027740
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzS140

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C4K29_RS19075
• Protein ID: WP_124305096.1
• Coordinates: 4264591..4264881 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: C4K29_RS19080
• Protein ID: WP_124305097.1
• Coordinates: 4264874..4265119 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K29_RS19045	4259742..4261058	+	1317	WP_124305090.1	ParB N-terminal domain-containing protein	-
C4K29_RS19050	4261058..4261618	+	561	WP_124305091.1	DUF2857 domain-containing protein	-
C4K29_RS19055	4261622..4262761	+	1140	WP_124305092.1	hypothetical protein	-
C4K29_RS19060	4262939..4263700	+	762	WP_124305093.1	TIGR03761 family integrating conjugative element protein	-
C4K29_RS19065	4263688..4264155	+	468	WP_124305094.1	DUF3158 family protein	-
C4K29_RS19070	4264152..4264553	+	402	WP_124305095.1	single-stranded DNA-binding protein	-
C4K29_RS19075	4264591..4264881	-	291	WP_124305096.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K29_RS19080	4264874..4265119	-	246	WP_124305097.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
C4K29_RS19085	4266349..4266651	+	303	WP_124305098.1	hypothetical protein	-
C4K29_RS19090	4266824..4267543	+	720	WP_124305099.1	ABC transporter substrate-binding protein	-
C4K29_RS19095	4267882..4268355	+	474	WP_124305100.1	DUF3577 domain-containing protein	-
C4K29_RS19100	4268416..4268724	+	309	WP_124305101.1	hypothetical protein	-
C4K29_RS19105	4268772..4269413	-	642	WP_124305102.1	glycoside hydrolase family 25 protein	-
C4K29_RS19110	4269565..4270056	+	492	WP_164485778.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	spaQ / invA	4146067..4301980	155913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-91)

Antitoxin

• Antitoxin ParD (7-80)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11415.08 Da
• Isoelectric Point: 7.8924

>T100114 WP_124305096.1 NZ_CP027740:c4264881-4264591 [Pseudomonas chlororaphis subsp. piscium]
MAEYHLAPAAERDLEGIWVYTVQRWNVEQAHRYIDIFTAAFSALAQSPRTFPACDYIRPGYRCSRIERHMVYFRVTDYGI
SIIRVLHDRMDALRYL

Nucleotide

• Length: 291 bp

>T100114 NZ_CP027740:c4264881-4264591 [Pseudomonas chlororaphis subsp. piscium]
ATGGCTGAGTATCATCTTGCACCGGCCGCTGAGCGTGACCTGGAGGGGATCTGGGTTTACACCGTTCAGCGGTGGAATGT
GGAGCAAGCGCATCGTTATATCGACATATTTACCGCCGCTTTTTCAGCTTTGGCGCAGTCGCCCAGAACATTCCCCGCCT
GCGACTACATCCGACCGGGCTATCGTTGCAGTCGGATTGAACGGCATATGGTTTATTTCCGCGTCACCGATTACGGGATT
TCAATCATCCGCGTATTGCATGATCGAATGGATGCGCTGCGTTATCTGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9108.15 Da
• Isoelectric Point: 5.8432

>AT100114 WP_124305097.1 NZ_CP027740:c4265119-4264874 [Pseudomonas chlororaphis subsp. piscium]
MGTVRKTITLTDTQDGWVKAQIDAGRFTNDSEYIRDLIRREQERSAEVDAIRAALIVGESSGEPRPFDPDAFKQRMSAKH
G

Nucleotide

• Length: 246 bp

>AT100114 NZ_CP027740:c4265119-4264874 [Pseudomonas chlororaphis subsp. piscium]
ATGGGAACAGTGCGTAAAACCATCACGCTGACTGACACACAGGACGGTTGGGTTAAAGCGCAAATTGATGCCGGCCGCTT
TACCAATGACAGCGAATACATCCGTGACCTGATCCGGCGGGAGCAGGAGCGCAGTGCTGAAGTGGACGCGATCCGTGCGG
CGCTAATTGTCGGTGAGTCCAGTGGCGAACCGCGCCCGTTTGACCCCGACGCCTTCAAGCAGAGAATGTCAGCCAAACAT
GGCTGA