Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2398188..2398773 Replicon chromosome
Accession NZ_CP027740
Organism Pseudomonas chlororaphis subsp. piscium strain ChPhzS140

Toxin (Protein)


Gene name PumA Uniprot ID A0A3G7C0N8
Locus tag C4K29_RS10950 Protein ID WP_025804099.1
Coordinates 2398480..2398773 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A3G7CJP2
Locus tag C4K29_RS10945 Protein ID WP_025804100.1
Coordinates 2398188..2398478 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K29_RS10930 2393513..2395993 + 2481 WP_124305345.1 FtsX-like permease family protein -
C4K29_RS10935 2395983..2397059 + 1077 WP_063429487.1 iron ABC transporter permease -
C4K29_RS10940 2397187..2397774 + 588 WP_063429489.1 hypothetical protein -
C4K29_RS10945 2398188..2398478 - 291 WP_025804100.1 putative addiction module antidote protein Antitoxin
C4K29_RS10950 2398480..2398773 - 294 WP_025804099.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4K29_RS10955 2399009..2400403 - 1395 WP_063429491.1 VOC family protein -
C4K29_RS10960 2400666..2401790 + 1125 WP_051519186.1 diguanylate cyclase -
C4K29_RS10965 2401884..2403128 + 1245 WP_025804096.1 M20/M25/M40 family metallo-hydrolase -
C4K29_RS10970 2403329..2403727 + 399 WP_025804095.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-96)

Antitoxin

Uncharacterized protein HI1420

(9-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10837.49 Da        Isoelectric Point: 10.7289

>T100113 WP_025804099.1 NZ_CP027740:c2398773-2398480 [Pseudomonas chlororaphis subsp. piscium]
MEYEILQTTVFAQWHTRLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNRGAGYRIYFTVRGNTLIVLLLGGDK
STQPADICQARSLAKEF

Download         Length: 294 bp

>T100113 NZ_CP027740:c2398773-2398480 [Pseudomonas chlororaphis subsp. piscium]
ATGGAATACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10249.81 Da        Isoelectric Point: 8.0096

>AT100113 WP_025804100.1 NZ_CP027740:c2398478-2398188 [Pseudomonas chlororaphis subsp. piscium]
MSDTLVPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Download         Length: 291 bp

>AT100113 NZ_CP027740:c2398478-2398188 [Pseudomonas chlororaphis subsp. piscium]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G7C0N8

Antitoxin

Source ID Structure
AlphaFold DB A0A3G7CJP2

References