Detailed information of TA system TA100097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 385983..387415
• Replicon: chromosome
• Accession: NZ_CP027738
• Organism: Pseudomonas chlororaphis subsp. piscium strain ChPhzS135

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: C4K31_RS01725
• Protein ID: WP_124325944.1
• Coordinates: 386327..387415 (-)
• Length: 363 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: C4K31_RS01720
• Protein ID: WP_124325943.1
• Coordinates: 385983..386312 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K31_RS01710	381473..383059	-	1587	WP_124325941.1	hypothetical protein	-
C4K31_RS01715	383005..385479	-	2475	WP_124325942.1	hypothetical protein	-
C4K31_RS01720	385983..386312	-	330	WP_124325943.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
C4K31_RS01725	386327..387415	-	1089	WP_124325944.1	type II toxin-antitoxin system RnlA family toxin	Toxin
C4K31_RS01730	387946..388221	-	276	WP_124325945.1	hypothetical protein	-
C4K31_RS01735	388218..389198	-	981	WP_124325946.1	integrase domain-containing protein	-
C4K31_RS01745	390103..391431	-	1329	WP_124325947.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	369537..389198	19661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (105-189)

Antitoxin

• Antitoxin RnlB/LsoB (14-109)

Sequences

Toxin

Protein

• Length: 363 a.a.
• Molecular weight: 40569.91 Da
• Isoelectric Point: 5.7844

>T100097 WP_124325944.1 NZ_CP027738:c387415-386327 [Pseudomonas chlororaphis subsp. piscium]
MEHNILEGRDYKDLNLNRDNIVPTVTSFAGSNALNIRNIVDRPGGPGMRVLIGKAGIEDATVDIYFTTTGSSTIQFKTGR
NHPLGEALADSLYETIHPDEFKSINLSLKGITFDDISAIISQLTEQPGAEVFIADHGKNKNSTMWKLKSKSNQDEITVHQ
HGTTNTLQIQGRPLSCYRELTYLTADLLDLNALECILFKKDDNRSEVIRSEVAEQFLSQKLGACVDRLPEATKKLLLSGL
CVQLASPALPDYCMLLYPELRSLEGAIRQRLAENSLEDQEDSFGCFFDKANGRFTLKQEYHSSIKDAKIVDILGDAYTFF
NKHRHGLFHMEAQPDGSRMLSNFTQLLTLSDKAYEHLTKLYS

Nucleotide

• Length: 1089 bp

>T100097 NZ_CP027738:c387415-386327 [Pseudomonas chlororaphis subsp. piscium]
ATGGAGCATAATATTTTGGAAGGCAGAGACTATAAAGATCTCAATCTGAATCGAGACAATATTGTACCAACAGTTACCTC
GTTCGCCGGCAGCAACGCACTAAATATTAGAAACATTGTTGATAGACCCGGAGGCCCCGGCATGAGGGTCTTAATTGGAA
AGGCTGGAATTGAAGACGCCACTGTAGACATCTATTTCACCACTACTGGCAGCTCTACCATCCAATTCAAAACTGGACGT
AACCATCCCTTAGGCGAAGCTCTTGCGGACTCTTTATACGAGACCATTCATCCAGACGAATTCAAGTCAATAAATCTTTC
ATTGAAAGGAATAACTTTCGACGACATTTCGGCCATAATATCTCAACTAACGGAACAGCCAGGCGCGGAAGTCTTCATCG
CAGATCACGGAAAAAACAAAAACTCCACAATGTGGAAGCTAAAATCAAAATCAAACCAAGATGAAATAACCGTACATCAG
CATGGGACTACTAACACCCTCCAAATTCAAGGAAGACCTCTCAGCTGCTACAGAGAGTTAACTTATCTCACTGCAGACCT
TTTAGACTTAAATGCACTAGAGTGCATACTATTCAAAAAAGACGACAATCGTTCCGAAGTAATTCGGTCGGAAGTCGCAG
AGCAGTTCCTATCCCAAAAGCTAGGAGCTTGCGTAGACCGGCTCCCCGAGGCGACAAAAAAACTACTTCTTTCAGGGCTG
TGTGTGCAGCTCGCAAGCCCAGCTTTACCTGACTACTGCATGCTTTTGTATCCCGAGCTTAGGTCGCTGGAGGGAGCCAT
TCGCCAGAGACTAGCCGAAAATAGCCTTGAGGATCAGGAAGATAGCTTCGGTTGTTTTTTCGACAAAGCCAACGGTCGGT
TCACTCTCAAGCAGGAATACCACAGTTCCATAAAAGATGCTAAAATTGTTGATATTCTCGGTGATGCTTATACGTTTTTT
AACAAGCATCGCCACGGTTTGTTTCACATGGAAGCACAACCAGATGGCAGCCGAATGCTAAGTAACTTCACTCAGTTATT
AACGCTTTCAGATAAAGCGTACGAACATCTGACGAAGCTTTACTCATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.74 Da
• Isoelectric Point: 5.4371

>AT100097 WP_124325943.1 NZ_CP027738:c386312-385983 [Pseudomonas chlororaphis subsp. piscium]
MFEIKRSDTSNGLKTVVIATSYENPLSSLSAIAKQMLSEHHTHTEEVIFDLLCANGDEWNRFAAIEYNGHSFVRSTFHIL
PPSSIDSKLIESQSHYFASHPEFLENSVL

Nucleotide

• Length: 330 bp

>AT100097 NZ_CP027738:c386312-385983 [Pseudomonas chlororaphis subsp. piscium]
ATGTTTGAGATTAAAAGATCAGACACTTCTAACGGCCTTAAAACTGTCGTTATAGCGACTAGTTATGAGAACCCGCTGTC
TAGTCTTTCTGCTATCGCAAAGCAAATGCTGAGCGAGCATCACACTCATACAGAGGAGGTCATATTTGATCTTCTATGCG
CCAATGGAGATGAATGGAATAGATTTGCTGCCATCGAGTATAATGGTCATAGCTTTGTTAGATCTACCTTTCATATTTTA
CCGCCAAGCTCCATCGACTCGAAATTAATAGAATCACAGTCTCATTACTTCGCATCACACCCTGAGTTTCTTGAGAACTC
AGTGCTCTGA