Detailed information of TA system TA100094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4159663..4160182
• Replicon: chromosome
• Accession: NZ_CP027737
• Organism: Pseudomonas chlororaphis subsp. piscium strain PCL1607

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4K32_RS18515
• Protein ID: WP_097304079.1
• Coordinates: 4159663..4159944 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4K32_RS18520
• Protein ID: WP_124348673.1
• Coordinates: 4159934..4160182 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K32_RS18495	4155551..4155787	-	237	WP_124348670.1	hypothetical protein	-
C4K32_RS18500	4155807..4156982	-	1176	WP_124348671.1	S-type pyocin domain-containing protein	-
C4K32_RS18505	4157308..4157790	+	483	WP_009049637.1	acyl-CoA thioesterase	-
C4K32_RS18510	4157847..4159496	+	1650	WP_124348672.1	FMN-binding glutamate synthase family protein	-
C4K32_RS18515	4159663..4159944	-	282	WP_097304079.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K32_RS18520	4159934..4160182	-	249	WP_124348673.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C4K32_RS18525	4160279..4161067	-	789	WP_124299848.1	helix-turn-helix transcriptional regulator	-
C4K32_RS18530	4161152..4162150	+	999	WP_124348674.1	bile acid:sodium symporter	-
C4K32_RS18535	4162364..4164127	+	1764	WP_124348675.1	hypothetical protein	-
C4K32_RS18540	4164201..4164662	+	462	WP_124348676.1	YccF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10863.71 Da
• Isoelectric Point: 10.6993

>T100094 WP_097304079.1 NZ_CP027737:c4159944-4159663 [Pseudomonas chlororaphis subsp. piscium]
MTYELEFSEKAWKEWQKLGPTLKEQFKKKLQERLVSPHVPADRLSGLGNAYKIKLRTAGYRLVYRVVDEVLVVTVIAVGK
RERGSVYQQARKR

Nucleotide

• Length: 282 bp

>T100094 NZ_CP027737:c4159944-4159663 [Pseudomonas chlororaphis subsp. piscium]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAGCCCCCATGTCCCGGCCGATCGCCTGAGCGGCTTGGGCAATGCCTACAAAATCA
AACTGCGCACGGCTGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGGGAGCGGGGGAGTGTCTATCAGCAAGCCAGGAAGCGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8996.39 Da
• Isoelectric Point: 4.5488

>AT100094 WP_124348673.1 NZ_CP027737:c4160182-4159934 [Pseudomonas chlororaphis subsp. piscium]
MTHVLLSNVVASISELKKNPMGTVAAGGGSSVAILNRNEPAFYCVPAKEYEAMMERLEDMELLALCRERENDPTIKVSLN
DL

Nucleotide

• Length: 249 bp

>AT100094 NZ_CP027737:c4160182-4159934 [Pseudomonas chlororaphis subsp. piscium]
ATGACCCATGTCCTTCTGTCCAACGTCGTTGCGAGCATTTCCGAGCTCAAGAAAAACCCCATGGGCACCGTTGCTGCCGG
TGGCGGTTCCTCCGTGGCCATACTCAATCGCAACGAACCGGCGTTTTACTGCGTTCCGGCCAAGGAATACGAAGCCATGA
TGGAGCGCCTGGAGGATATGGAACTGCTCGCCTTGTGCCGTGAGCGAGAGAATGATCCGACCATCAAGGTTTCGCTCAAT
GACTTATGA