Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HTH_37(antitoxin)
Location 6451798..6452438 Replicon chromosome
Accession NZ_CP027735
Organism Pseudomonas chlororaphis subsp. piscium strain DTR133

Toxin (Protein)


Gene name hicA Uniprot ID A0A285GR45
Locus tag C4K34_RS28930 Protein ID WP_028681531.1
Coordinates 6451798..6451980 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PIE8
Locus tag C4K34_RS28935 Protein ID WP_041984783.1
Coordinates 6452013..6452438 (+) Length 142 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K34_RS28900 6447114..6447824 + 711 WP_124372868.1 hypothetical protein -
C4K34_RS28905 6447821..6448744 + 924 WP_124372892.1 hypothetical protein -
C4K34_RS28910 6448741..6449289 + 549 WP_096375630.1 DNA-binding protein -
C4K34_RS28915 6449286..6449990 + 705 WP_124372869.1 hypothetical protein -
C4K34_RS28920 6449963..6450934 + 972 WP_124372870.1 hypothetical protein -
C4K34_RS28925 6450931..6451572 - 642 WP_124372871.1 hypothetical protein -
C4K34_RS28930 6451798..6451980 + 183 WP_028681531.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K34_RS28935 6452013..6452438 + 426 WP_041984783.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K34_RS28940 6452984..6453175 + 192 WP_124372872.1 hypothetical protein -
C4K34_RS28945 6453608..6454411 + 804 WP_063433210.1 hypothetical protein -
C4K34_RS28950 6454499..6456865 - 2367 WP_063433211.1 glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 6412431..6452438 40007


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6764.83 Da        Isoelectric Point: 10.7304

>T100083 WP_028681531.1 NZ_CP027735:6451798-6451980 [Pseudomonas chlororaphis subsp. piscium]
MKYSEFRRWLKAQGAEFQPGKGSHFKVTLNGKSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Download         Length: 183 bp

>T100083 NZ_CP027735:6451798-6451980 [Pseudomonas chlororaphis subsp. piscium]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGCGGAGTTCCAACCCGGCAAGGGCAGTCACTTCAAGGT
CACCTTGAATGGCAAGTCAACCGTGTTTCCAGACCACGGAGCCAAGGAAATGGGCGAAGGGTTAAGGAAGTCGATAATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 142 a.a.        Molecular weight: 15470.58 Da        Isoelectric Point: 4.4313

>AT100083 WP_041984783.1 NZ_CP027735:6452013-6452438 [Pseudomonas chlororaphis subsp. piscium]
MFKYALEIHEEPGTVWLSCEEIPEFHAAGDTVGEALDSVLDALETALSIYVDERRPIPSGNAEAQAGHVLLCLPALTAAK
VALWNAVLDEGTTKAELARRLGIQRPQVDRLLDFLHDSKIENVERALQELGRRISITVEAA

Download         Length: 426 bp

>AT100083 NZ_CP027735:6452013-6452438 [Pseudomonas chlororaphis subsp. piscium]
ATGTTCAAGTACGCATTGGAAATTCACGAAGAGCCCGGCACCGTCTGGCTTTCGTGTGAGGAGATTCCAGAATTTCACGC
TGCCGGCGACACCGTAGGCGAGGCGCTGGATAGTGTTTTGGATGCGCTCGAAACGGCTCTGTCTATCTATGTCGATGAAC
GCCGCCCGATTCCATCTGGTAACGCCGAGGCTCAGGCAGGGCACGTTCTGTTATGCCTTCCAGCCCTCACCGCGGCGAAG
GTTGCACTGTGGAACGCGGTGTTGGATGAAGGCACCACCAAGGCAGAGCTGGCGCGCCGCCTGGGGATTCAGCGGCCGCA
GGTGGACCGCTTGCTGGACTTCCTGCACGACTCGAAAATCGAGAACGTCGAGCGCGCCCTGCAGGAACTCGGTCGGCGGA
TCTCTATCACCGTCGAAGCTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285GR45

Antitoxin

Source ID Structure
AlphaFold DB A0A554PIE8

References