Detailed information of TA system TA100082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2428664..2429249
• Replicon: chromosome
• Accession: NZ_CP027735
• Organism: Pseudomonas chlororaphis subsp. piscium strain DTR133

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C4K34_RS11165
• Protein ID: WP_124372621.1
• Coordinates: 2428956..2429249 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7CJP2
• Locus tag: C4K34_RS11160
• Protein ID: WP_025804100.1
• Coordinates: 2428664..2428954 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4K34_RS11145	2423989..2426469	+	2481	WP_124372618.1	FtsX-like permease family protein	-
C4K34_RS11150	2426459..2427535	+	1077	WP_124372619.1	iron ABC transporter permease	-
C4K34_RS11155	2427663..2428250	+	588	WP_124372620.1	hypothetical protein	-
C4K34_RS11160	2428664..2428954	-	291	WP_025804100.1	putative addiction module antidote protein	Antitoxin
C4K34_RS11165	2428956..2429249	-	294	WP_124372621.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4K34_RS11170	2429485..2430879	-	1395	WP_124372622.1	VOC family protein	-
C4K34_RS11175	2431142..2432266	+	1125	WP_124372623.1	diguanylate cyclase	-
C4K34_RS11180	2432360..2433604	+	1245	WP_124372624.1	M20/M25/M40 family metallo-hydrolase	-
C4K34_RS11185	2433817..2434215	+	399	WP_124372625.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (41-83)

Antitoxin

• Uncharacterized protein HI1420 (9-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10851.52 Da
• Isoelectric Point: 10.7289

>T100082 WP_124372621.1 NZ_CP027735:c2429249-2428956 [Pseudomonas chlororaphis subsp. piscium]
MDYEILQTTVFAQWHTRLRDLRVRIAIARRIERASAGNLGDVKNLGGGLSEMRVNRGAGYRIYFTVRGNTLIVLLLGGDK
STQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T100082 NZ_CP027735:c2429249-2428956 [Pseudomonas chlororaphis subsp. piscium]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCAGACTACGGGACCTGCGCGTCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATAGGGGCGCCGGCTACCGCATCTATTTCACGGTTCGCGGCAACACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCCACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10249.81 Da
• Isoelectric Point: 8.0096

>AT100082 WP_025804100.1 NZ_CP027735:c2428954-2428664 [Pseudomonas chlororaphis subsp. piscium]
MSDTLVPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Nucleotide

• Length: 291 bp

>AT100082 NZ_CP027735:c2428954-2428664 [Pseudomonas chlororaphis subsp. piscium]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCGCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCCTTTGCCCCGGGCGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGTTGTGCACCACAGTGCCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7CJP2