Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1601347..1601969 Replicon chromosome
Accession NZ_CP027735
Organism Pseudomonas chlororaphis subsp. piscium strain DTR133

Toxin (Protein)


Gene name hicA Uniprot ID A0A2N8AR08
Locus tag C4K34_RS07420 Protein ID WP_007929414.1
Coordinates 1601787..1601969 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2N8AQX2
Locus tag C4K34_RS07415 Protein ID WP_063429381.1
Coordinates 1601347..1601754 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4K34_RS07395 1596649..1597542 + 894 WP_173678573.1 LysR family transcriptional regulator -
C4K34_RS07400 1597778..1599556 + 1779 WP_063428837.1 GGDEF and EAL domain-containing protein -
C4K34_RS07405 1599590..1600504 - 915 WP_063428839.1 SGNH/GDSL hydrolase family protein -
C4K34_RS07410 1600634..1601101 - 468 WP_063428841.1 GAF domain-containing protein -
C4K34_RS07415 1601347..1601754 - 408 WP_063429381.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4K34_RS07420 1601787..1601969 - 183 WP_007929414.1 type II toxin-antitoxin system HicA family toxin Toxin
C4K34_RS07430 1602688..1603572 - 885 WP_009047472.1 alpha/beta hydrolase -
C4K34_RS07435 1603924..1604616 - 693 WP_025804997.1 16S rRNA pseudouridine(516) synthase -
C4K34_RS07440 1604650..1604883 - 234 WP_036997815.1 cysteine-rich CWC family protein -
C4K34_RS07445 1604876..1606363 - 1488 WP_025804999.1 sensor domain-containing diguanylate cyclase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6850.15 Da        Isoelectric Point: 11.6709

>T100081 WP_007929414.1 NZ_CP027735:c1601969-1601787 [Pseudomonas chlororaphis subsp. piscium]
VNSRYLIGQIVADGWYLVRVRGSHHHFRHPSKPGLVTVPHPKKDLLRKTAISILKQALLM

Download         Length: 183 bp

>T100081 NZ_CP027735:c1601969-1601787 [Pseudomonas chlororaphis subsp. piscium]
GTGAACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCACCATTT
CAGGCACCCCAGTAAACCGGGGCTGGTGACAGTTCCACATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14604.60 Da        Isoelectric Point: 4.3638

>AT100081 WP_063429381.1 NZ_CP027735:c1601754-1601347 [Pseudomonas chlororaphis subsp. piscium]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAAIPPANKVTLHAANPQYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Download         Length: 408 bp

>AT100081 NZ_CP027735:c1601754-1601347 [Pseudomonas chlororaphis subsp. piscium]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGCGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGCGCCGCCATCCCACCCGCCAACAAGGTCACCCTGCACGCGGCCAACCCTCAGTATGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCAAAAAGCCGCTCGGGTTTTCTCGCTTCGGCTGCATTGAAGGTATTGCAGC
AGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8AR08

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8AQX2

References