Detailed information of TA system TA100074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2369329..2369942
• Replicon: chromosome
• Accession: NZ_CP027734
• Organism: Pseudomonas sp. R1-43-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4U3I3Z1
• Locus tag: C4J87_RS10910
• Protein ID: WP_057722035.1
• Coordinates: 2369691..2369942 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J87_RS10905
• Protein ID: WP_124366775.1
• Coordinates: 2369329..2369694 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J87_RS26200	2365332..2366552	+	1221	WP_177412792.1	hypothetical protein	-
C4J87_RS10895	2366762..2368117	+	1356	WP_124366773.1	MATE family efflux transporter	-
C4J87_RS10900	2368157..2369362	+	1206	WP_124366774.1	MFS transporter	-
C4J87_RS10905	2369329..2369694	-	366	WP_124366775.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J87_RS10910	2369691..2369942	-	252	WP_057722035.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9490.04 Da
• Isoelectric Point: 10.0459

>T100074 WP_057722035.1 NZ_CP027734:c2369942-2369691 [Pseudomonas sp. R1-43-08]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100074 NZ_CP027734:c2369942-2369691 [Pseudomonas sp. R1-43-08]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAACTCGTAACCCTGTT
ACGGCGACTGGGTTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAGTGCACTGATCA
CCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13211.06 Da
• Isoelectric Point: 6.6267

>AT100074 WP_124366775.1 NZ_CP027734:c2369694-2369329 [Pseudomonas sp. R1-43-08]
VNNQLKHKGYIGSIEASLEDNCLFGKVLFIKALVSYEGKTVAELDAAFQEAVDDYLSTCQSLGQVPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLQHHFPQPCAAIG

Nucleotide

• Length: 366 bp

>AT100074 NZ_CP027734:c2369694-2369329 [Pseudomonas sp. R1-43-08]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAGGT
CCTGTTCATCAAGGCCCTGGTGAGCTACGAAGGCAAAACCGTCGCAGAGTTGGATGCGGCCTTCCAAGAGGCGGTGGACG
ATTACCTCTCCACCTGCCAGTCGCTCGGGCAAGTGCCTGAAAAGCCCTGCAAGGGCTCATTCAACGTACGCGTCGGCCAT
GACCTGCATCTGGCGGCAGCGTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACACGCCAGGCGCTGGATGA
GTTTCTACAGCATCATTTCCCGCAGCCTTGCGCGGCGATTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A4U3I3Z1