Detailed information of TA system TA100071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1564486..1565008
• Replicon: chromosome
• Accession: NZ_CP027734
• Organism: Pseudomonas sp. R1-43-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J87_RS07095
• Protein ID: WP_124366221.1
• Coordinates: 1564486..1564779 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J87_RS07100
• Protein ID: WP_124366222.1
• Coordinates: 1564769..1565008 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J87_RS07065	1559879..1560439	-	561	WP_003189463.1	glycine cleavage system protein R	-
C4J87_RS07070	1560702..1561580	+	879	WP_124366220.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C4J87_RS07075	1561598..1562713	+	1116	WP_124361739.1	outer membrane protein assembly factor BamC	-
C4J87_RS07080	1562718..1563476	+	759	WP_057723051.1	MBL fold metallo-hydrolase	-
C4J87_RS07085	1563504..1564217	+	714	WP_048726015.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C4J87_RS07095	1564486..1564779	-	294	WP_124366221.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J87_RS07100	1564769..1565008	-	240	WP_124366222.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C4J87_RS07110	1565475..1566701	-	1227	WP_124366224.1	MFS transporter	-
C4J87_RS07115	1566921..1567238	-	318	WP_124366225.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4J87_RS07120	1567235..1567516	-	282	WP_177412808.1	type II toxin-antitoxin system ParD family antitoxin	-
C4J87_RS07125	1567850..1568752	+	903	WP_065886461.1	LysR family transcriptional regulator	-
C4J87_RS07130	1568744..1569502	-	759	WP_124366227.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11634.49 Da
• Isoelectric Point: 10.2263

>T100071 WP_124366221.1 NZ_CP027734:c1564779-1564486 [Pseudomonas sp. R1-43-08]
MIYSLDFDARALKEWQKLGDTLRQQFKKKLVKILNNPHIDANRLHGLPDCYKIKLRSSGYRLVYQVIDQEITVFVVAVDK
REHDQAYRKAFERLDRR

Nucleotide

• Length: 294 bp

>T100071 NZ_CP027734:c1564779-1564486 [Pseudomonas sp. R1-43-08]
ATGATCTATAGCCTTGATTTTGATGCGCGTGCATTGAAGGAATGGCAAAAACTCGGCGACACCCTTCGTCAGCAATTCAA
GAAGAAGCTGGTCAAAATCCTGAACAACCCACACATTGACGCGAACCGCCTGCATGGCCTGCCTGACTGCTACAAGATCA
AACTGCGTAGCAGCGGGTACAGGCTGGTCTATCAAGTGATCGATCAGGAAATTACTGTGTTTGTGGTCGCCGTTGATAAA
CGTGAGCATGATCAGGCTTATCGGAAAGCGTTTGAGCGCTTGGACCGGCGCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8951.35 Da
• Isoelectric Point: 4.6017

>AT100071 WP_124366222.1 NZ_CP027734:c1565008-1564769 [Pseudomonas sp. R1-43-08]
MASINIRIDDDLKARAYLELEKLGVTPSELIRQALQYVAERGQLPFKPVLMTEEDEALIATVRERLASPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT100071 NZ_CP027734:c1565008-1564769 [Pseudomonas sp. R1-43-08]
TTGGCCTCTATCAACATTCGTATCGATGACGACCTTAAGGCTCGCGCCTACCTTGAACTGGAAAAACTCGGTGTCACGCC
TTCCGAACTGATACGCCAGGCGCTGCAATATGTAGCCGAGCGCGGCCAACTGCCATTCAAGCCCGTGCTGATGACTGAAG
AAGATGAAGCCTTGATAGCGACCGTGCGTGAGCGCCTTGCGTCGCCTCAACGAGTGAAGGTTTCTTTAGATGATCTATAG