Detailed information of TA system TA100070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1532746..1533259
• Replicon: chromosome
• Accession: NZ_CP027734
• Organism: Pseudomonas sp. R1-43-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J87_RS06895
• Protein ID: WP_124361707.1
• Coordinates: 1532746..1533030 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J87_RS06900
• Protein ID: WP_124361708.1
• Coordinates: 1533020..1533259 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J87_RS06870	1528058..1528864	-	807	WP_124366191.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
C4J87_RS06875	1529217..1529891	+	675	WP_124366192.1	NAD-dependent epimerase/dehydratase family protein	-
C4J87_RS06880	1529984..1531177	+	1194	WP_124366193.1	CoA transferase	-
C4J87_RS06885	1531264..1531752	-	489	WP_124366194.1	DMT family transporter	-
C4J87_RS06890	1531821..1532738	+	918	WP_124366195.1	LysR family transcriptional regulator	-
C4J87_RS06895	1532746..1533030	-	285	WP_124361707.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J87_RS06900	1533020..1533259	-	240	WP_124361708.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4J87_RS06905	1533360..1533770	+	411	WP_124366196.1	fosfomycin resistance glutathione transferase	-
C4J87_RS06910	1533940..1534467	+	528	WP_057723086.1	DUF4142 domain-containing protein	-
C4J87_RS06915	1534521..1534946	-	426	WP_124366197.1	low affinity iron permease family protein	-
C4J87_RS06920	1535123..1535905	+	783	WP_124366198.1	GNAT family N-acetyltransferase	-
C4J87_RS06925	1535906..1536406	-	501	WP_124366199.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4J87_RS06930	1536506..1536838	-	333	WP_124366200.1	hypothetical protein	-
C4J87_RS06940	1537243..1538127	-	885	WP_124366201.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11014.73 Da
• Isoelectric Point: 10.1506

>T100070 WP_124361707.1 NZ_CP027734:c1533030-1532746 [Pseudomonas sp. R1-43-08]
MQVEWLRTALRNLDDEAAFIAEENPKAAYEFVQAILAGLEHIAQFPALGKTGRVPGTREWVVPRWPYIVPYRIREGRLQV
MRIFHTRRQPPTSW

Nucleotide

• Length: 285 bp

>T100070 NZ_CP027734:c1533030-1532746 [Pseudomonas sp. R1-43-08]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGAAACCTGGATGATGAAGCGGCCTTTATTGCTGAAGAAAACCCCAAAGC
CGCCTACGAGTTCGTCCAGGCGATTTTGGCCGGGCTTGAACACATTGCGCAATTTCCCGCGTTGGGAAAAACCGGGCGCG
TCCCAGGGACGCGTGAATGGGTGGTGCCCCGGTGGCCCTACATTGTCCCTTACCGTATTCGTGAAGGGCGACTACAGGTG
ATGCGAATTTTTCATACCCGCCGCCAACCACCGACCTCCTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8597.72 Da
• Isoelectric Point: 4.7571

>AT100070 WP_124361708.1 NZ_CP027734:c1533259-1533020 [Pseudomonas sp. R1-43-08]
MSVMSLRIPDDVADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALKEADAGDFATAAEVKAVADKWRNNAG

Nucleotide

• Length: 240 bp

>AT100070 NZ_CP027734:c1533259-1533020 [Pseudomonas sp. R1-43-08]
ATGTCTGTTATGTCCCTGCGGATACCGGACGATGTCGCCGATACGCTTGCCAGTCTGGCCAAAGCGACCGGTCGCAGCAA
ATCGTTCCTCGCTGTTGACGCGCTACGTGAATACTTGACGCGTGAAGCATGGCAAATCGAAGAGATTCAAAAGGCGCTGA
AAGAAGCTGATGCGGGGGATTTCGCGACGGCTGCCGAGGTAAAAGCCGTCGCTGACAAATGGCGCAACAATGCAGGTTGA