Detailed information of TA system TA100064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1836127..1836731
• Replicon: chromosome
• Accession: NZ_CP027733
• Organism: Pseudomonas sp. R4-39-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G8C3H2
• Locus tag: C4J88_RS08420
• Protein ID: WP_057722727.1
• Coordinates: 1836127..1836315 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J88_RS08425
• Protein ID: WP_048729935.1
• Coordinates: 1836327..1836731 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J88_RS08395	1831986..1832522	+	537	WP_124361949.1	DsbE family thiol:disulfide interchange protein	-
C4J88_RS08400	1832519..1832992	+	474	WP_124366413.1	cytochrome c-type biogenesis protein CcmH	-
C4J88_RS08405	1832985..1834169	+	1185	WP_124406709.1	c-type cytochrome biogenesis protein CcmI	-
C4J88_RS08410	1834185..1834580	+	396	WP_124370154.1	hypothetical protein	-
C4J88_RS08415	1835380..1835946	+	567	WP_124406710.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J88_RS08420	1836127..1836315	+	189	WP_057722727.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J88_RS08425	1836327..1836731	+	405	WP_048729935.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J88_RS08430	1836728..1837924	-	1197	WP_124406711.1	MFS transporter	-
C4J88_RS08435	1838149..1839153	-	1005	WP_124406712.1	sulfate ABC transporter substrate-binding protein	-
C4J88_RS08440	1839250..1840074	-	825	WP_124361959.1	ion transporter	-
C4J88_RS08445	1840106..1840981	-	876	WP_124406713.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7204.45 Da
• Isoelectric Point: 11.2000

>T100064 WP_057722727.1 NZ_CP027733:1836127-1836315 [Pseudomonas sp. R4-39-08]
VQSRLLIKELLEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100064 NZ_CP027733:1836127-1836315 [Pseudomonas sp. R4-39-08]
GTGCAAAGCAGGCTATTGATCAAGGAATTGCTGGAAGCGGGTTGGGTGCTGGATCGCGTCACCGGCAGCCATCACATTTT
TACCCACCGCTATAACCCGTACACGATTCCGGTGCCTCATCCGAAGAAGGATCTGCCTTTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14703.69 Da
• Isoelectric Point: 4.6000

>AT100064 WP_048729935.1 NZ_CP027733:1836327-1836731 [Pseudomonas sp. R4-39-08]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100064 NZ_CP027733:1836327-1836731 [Pseudomonas sp. R4-39-08]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGGAGTGATTCCGATGCCAACCTCAGTCGCCAATCATCACGCCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAGCTGGACATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTATGTCATCCAACGCAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCTTTGGAGAAGTTGGTGCGGT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A3G8C3H2