Detailed information of TA system TA100062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1501082..1501595
• Replicon: chromosome
• Accession: NZ_CP027733
• Organism: Pseudomonas sp. R4-39-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J88_RS06680
• Protein ID: WP_124361707.1
• Coordinates: 1501082..1501366 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J88_RS06685
• Protein ID: WP_124361708.1
• Coordinates: 1501356..1501595 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J88_RS06655	1496394..1497200	-	807	WP_124366191.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
C4J88_RS06660	1497553..1498227	+	675	WP_124406515.1	NAD-dependent epimerase/dehydratase family protein	-
C4J88_RS06665	1498320..1499513	+	1194	WP_124406516.1	CoA transferase	-
C4J88_RS06670	1499600..1500088	-	489	WP_124369938.1	DMT family transporter	-
C4J88_RS06675	1500166..1501074	+	909	WP_177412903.1	LysR family transcriptional regulator	-
C4J88_RS06680	1501082..1501366	-	285	WP_124361707.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J88_RS06685	1501356..1501595	-	240	WP_124361708.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4J88_RS06690	1501696..1502106	+	411	WP_124406518.1	fosfomycin resistance glutathione transferase	-
C4J88_RS06695	1502276..1502803	+	528	WP_057723086.1	DUF4142 domain-containing protein	-
C4J88_RS06700	1502856..1503278	-	423	WP_124406519.1	low affinity iron permease family protein	-
C4J88_RS06705	1503455..1504237	+	783	WP_124406520.1	GNAT family N-acetyltransferase	-
C4J88_RS06710	1504238..1504738	-	501	WP_124361714.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4J88_RS06715	1504838..1505170	-	333	WP_124406521.1	hypothetical protein	-
C4J88_RS06725	1505575..1506459	-	885	WP_124406522.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11014.73 Da
• Isoelectric Point: 10.1506

>T100062 WP_124361707.1 NZ_CP027733:c1501366-1501082 [Pseudomonas sp. R4-39-08]
MQVEWLRTALRNLDDEAAFIAEENPKAAYEFVQAILAGLEHIAQFPALGKTGRVPGTREWVVPRWPYIVPYRIREGRLQV
MRIFHTRRQPPTSW

Nucleotide

• Length: 285 bp

>T100062 NZ_CP027733:c1501366-1501082 [Pseudomonas sp. R4-39-08]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGAAACCTGGATGATGAAGCGGCCTTTATTGCTGAAGAAAACCCCAAAGC
CGCCTACGAGTTCGTCCAGGCGATTTTGGCCGGGCTTGAACACATTGCGCAATTTCCCGCGTTGGGAAAAACCGGGCGCG
TCCCAGGGACGCGTGAATGGGTGGTGCCCCGGTGGCCCTACATTGTCCCTTACCGTATTCGTGAAGGGCGACTACAGGTG
ATGCGAATTTTTCATACCCGCCGCCAACCACCTACCTCCTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8597.72 Da
• Isoelectric Point: 4.7571

>AT100062 WP_124361708.1 NZ_CP027733:c1501595-1501356 [Pseudomonas sp. R4-39-08]
MSVMSLRIPDDVADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALKEADAGDFATAAEVKAVADKWRNNAG

Nucleotide

• Length: 240 bp

>AT100062 NZ_CP027733:c1501595-1501356 [Pseudomonas sp. R4-39-08]
ATGTCTGTTATGTCCCTGCGGATACCGGACGATGTCGCCGATACGCTTGCCAGTCTGGCCAAAGCGACCGGTCGCAGCAA
ATCGTTCCTCGCTGTTGACGCGCTACGTGAATACTTGACGCGTGAAGCATGGCAAATCGAAGAGATTCAAAAGGCGCTGA
AAGAAGCTGATGCGGGGGATTTCGCGACGGCTGCCGAGGTAAAAGCCGTCGCTGACAAATGGCGCAACAATGCAGGTTGA