Detailed information of TA system TA100060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2581770..2582383
• Replicon: chromosome
• Accession: NZ_CP027732
• Organism: Pseudomonas sp. R4-35-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J89_RS11945
• Protein ID: WP_124414503.1
• Coordinates: 2582132..2582383 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J89_RS11940
• Protein ID: WP_124362545.1
• Coordinates: 2581770..2582135 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J89_RS27000	2577736..2579040	+	1305	WP_177413009.1	hypothetical protein	-
C4J89_RS11930	2579203..2580558	+	1356	WP_124362543.1	MATE family efflux transporter	-
C4J89_RS11935	2580598..2581803	+	1206	WP_124414502.1	MFS transporter	-
C4J89_RS11940	2581770..2582135	-	366	WP_124362545.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J89_RS11945	2582132..2582383	-	252	WP_124414503.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9564.09 Da
• Isoelectric Point: 8.5241

>T100060 WP_124414503.1 NZ_CP027732:c2582383-2582132 [Pseudomonas sp. R4-35-07]
VSKNEKLLAKLLNEHMAFTWSELVTLLSRLGYTQIEEAGSRVKFDIGDPNAMITLHKPYPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100060 NZ_CP027732:c2582383-2582132 [Pseudomonas sp. R4-35-07]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAACTCGTAACCCTGTT
AAGTCGACTGGGTTACACCCAGATTGAAGAGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAATGCAATGATCA
CCTTGCACAAGCCTTACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13208.01 Da
• Isoelectric Point: 6.7400

>AT100060 WP_124362545.1 NZ_CP027732:c2582135-2581770 [Pseudomonas sp. R4-35-07]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLHHHFPQPCAATG

Nucleotide

• Length: 366 bp

>AT100060 NZ_CP027732:c2582135-2581770 [Pseudomonas sp. R4-35-07]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTGGTGAGCTACGAAGGCAAAACCGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCGGTGGACG
ATTACCTCGCCACCTGCCAGTCGCTCGGGCAAACTCCTGAAAAGCCATGCAAGGGCTCGTTCAACGTACGCGTCGGCCAT
GACCTGCATCTGGCGGCAGCGTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACACGCCAGGCGCTGGATGA
GTTTCTACACCATCATTTCCCGCAGCCTTGCGCGGCGACTGGCTGA