Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PhRel-ATphRel/- |
| Location | 25126..26227 | Replicon | chromosome |
| Accession | NC_031266 | ||
| Organism | Mycobacterium phage Phrann | ||
Toxin (Protein)
| Gene name | phRel | Uniprot ID | A0A142K822 |
| Locus tag | BJD68_gp29 | Protein ID | YP_009304221.1 |
| Coordinates | 25126..26004 (+) | Length | 293 a.a. |
Antitoxin (Protein)
| Gene name | ATphRel | Uniprot ID | A0A142K823 |
| Locus tag | BJD68_gp30 | Protein ID | YP_009304222.1 |
| Coordinates | 26012..26227 (+) | Length | 72 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BJD68_gp21 (SEA_PHRANN_21) | 20409..21479 | + | 1071 | YP_009304213.1 | minor tail protein | - |
| BJD68_gp22 (SEA_PHRANN_22) | 21476..21652 | + | 177 | YP_009304214.1 | hypothetical protein | - |
| BJD68_gp23 (SEA_PHRANN_23) | 21664..22026 | + | 363 | YP_009304215.1 | hypothetical protein | - |
| BJD68_gp24 (SEA_PHRANN_24) | 22023..22196 | + | 174 | YP_009304216.1 | hypothetical protein | - |
| BJD68_gp25 (SEA_PHRANN_25) | 22267..23202 | + | 936 | YP_009304217.1 | endolysin | - |
| BJD68_gp26 (SEA_PHRANN_26) | 23199..24365 | + | 1167 | YP_009304218.1 | endolysin | - |
| BJD68_gp27 (SEA_PHRANN_27) | 24392..24637 | + | 246 | YP_009304219.1 | holin | - |
| BJD68_gp28 (SEA_PHRANN_28) | 24634..24999 | + | 366 | YP_009304220.1 | minor tail protein | - |
| BJD68_gp29 (SEA_PHRANN_29) | 25126..26004 | + | 879 | YP_009304221.1 | hypothetical protein | Toxin |
| BJD68_gp30 (SEA_PHRANN_30) | 26012..26227 | + | 216 | YP_009304222.1 | hypothetical protein | Antitoxin |
| BJD68_gp31 (SEA_PHRANN_31) | 26339..26566 | + | 228 | YP_009304223.1 | hypothetical protein | - |
| BJD68_gp32 (SEA_PHRANN_32) | 26572..27258 | - | 687 | YP_009304224.1 | hypothetical protein | - |
| BJD68_gp33 (SEA_PHRANN_33) | 27355..27801 | - | 447 | YP_009304225.1 | HicB-like antitoxin | - |
| BJD68_gp34 (SEA_PHRANN_34) | 27798..28016 | - | 219 | YP_009304226.1 | toxin | - |
| BJD68_gp35 (SEA_PHRANN_35) | 28062..28271 | - | 210 | YP_009304227.1 | hypothetical protein | - |
| BJD68_gp36 (SEA_PHRANN_36) | 28461..28799 | - | 339 | YP_009304228.1 | DUF2510 domain-containing protein | - |
| BJD68_gp37 (SEA_PHRANN_37) | 28845..29816 | - | 972 | YP_009304229.1 | integrase | - |
| BJD68_gp38 (SEA_PHRANN_38) | 29820..30191 | - | 372 | YP_009304230.1 | transcriptional repressor | - |
| BJD68_gp39 (SEA_PHRANN_39) | 30325..30558 | + | 234 | YP_009304231.1 | transcriptional repressor | - |
| BJD68_gp40 (SEA_PHRANN_40) | 30555..30902 | + | 348 | YP_009304232.1 | hypothetical protein | - |
| BJD68_gp41 (SEA_PHRANN_41) | 30899..31072 | + | 174 | YP_009304233.1 | membrane protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 96..44399 | 44303 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 293 a.a. Molecular weight: 33418.56 Da Isoelectric Point: 6.2493
>T10006 YP_009304221.1 NC_031266:25126-26004 [Mycobacterium phage Phrann]
VENRPSSIINVAPAWSKGELKRLGEALFSGTATPEQHARYNEVMLWHNELAAEVAATLYTTDWQACPSELFDITARPKTI
DTLIQKLQRERSMSLDEVQDLAGVRIDADIDLKVQTALAEEIATHFGERSRIRDMRENPHSGYRAVHVWLRLPAGRVEVQ
IRTVPQSEWANTYERLGDRYGRGIRYGASAEDDEARELVERMHKMSAALASSEESTVELAQLEDELHQLKERLAEMRPLA
RASGPINHRRVSKALDRTFRKAEETRTRLNQHRDSYLQMLREMRSMLDTDGS
VENRPSSIINVAPAWSKGELKRLGEALFSGTATPEQHARYNEVMLWHNELAAEVAATLYTTDWQACPSELFDITARPKTI
DTLIQKLQRERSMSLDEVQDLAGVRIDADIDLKVQTALAEEIATHFGERSRIRDMRENPHSGYRAVHVWLRLPAGRVEVQ
IRTVPQSEWANTYERLGDRYGRGIRYGASAEDDEARELVERMHKMSAALASSEESTVELAQLEDELHQLKERLAEMRPLA
RASGPINHRRVSKALDRTFRKAEETRTRLNQHRDSYLQMLREMRSMLDTDGS
Download Length: 879 bp
>T10006 NC_031266:25126-26004 [Mycobacterium phage Phrann]
GTGGAGAATCGCCCATCGAGCATCATCAACGTGGCGCCGGCGTGGAGCAAAGGTGAGCTGAAACGGCTCGGGGAAGCTCT
TTTCAGCGGTACCGCTACACCCGAACAGCACGCCAGATACAACGAGGTGATGCTGTGGCACAACGAGCTGGCCGCCGAGG
TCGCCGCCACGCTCTACACGACCGATTGGCAAGCCTGCCCCTCTGAATTGTTTGACATCACCGCTCGCCCCAAAACCATC
GACACGCTGATTCAGAAGCTGCAGCGAGAACGCAGCATGAGCCTCGACGAGGTCCAAGACCTGGCGGGTGTGCGAATCGA
CGCCGACATCGATCTCAAGGTTCAGACGGCACTCGCCGAGGAGATCGCCACACACTTCGGGGAACGGTCTCGGATACGCG
ATATGCGAGAAAACCCCCATAGTGGCTATCGTGCCGTCCACGTCTGGCTGCGACTGCCGGCCGGCCGCGTGGAGGTCCAG
ATCCGAACAGTGCCTCAGAGCGAATGGGCGAACACCTACGAGCGACTGGGCGACCGGTACGGTCGAGGTATCCGCTACGG
CGCGTCGGCAGAGGACGATGAAGCGCGCGAGCTGGTCGAGAGAATGCACAAGATGTCCGCGGCGTTAGCAAGTAGTGAGG
AGTCCACGGTCGAGTTGGCCCAACTCGAAGACGAGCTCCATCAGCTAAAGGAAAGGCTGGCGGAGATGCGGCCGCTCGCG
AGGGCGTCGGGGCCGATTAATCACCGGAGAGTGTCGAAGGCGCTGGATAGGACCTTCCGCAAGGCCGAGGAGACAAGGAC
GCGATTGAACCAGCACCGGGATTCGTACTTGCAAATGCTCCGCGAGATGCGCAGTATGCTCGATACAGACGGGAGTTGA
GTGGAGAATCGCCCATCGAGCATCATCAACGTGGCGCCGGCGTGGAGCAAAGGTGAGCTGAAACGGCTCGGGGAAGCTCT
TTTCAGCGGTACCGCTACACCCGAACAGCACGCCAGATACAACGAGGTGATGCTGTGGCACAACGAGCTGGCCGCCGAGG
TCGCCGCCACGCTCTACACGACCGATTGGCAAGCCTGCCCCTCTGAATTGTTTGACATCACCGCTCGCCCCAAAACCATC
GACACGCTGATTCAGAAGCTGCAGCGAGAACGCAGCATGAGCCTCGACGAGGTCCAAGACCTGGCGGGTGTGCGAATCGA
CGCCGACATCGATCTCAAGGTTCAGACGGCACTCGCCGAGGAGATCGCCACACACTTCGGGGAACGGTCTCGGATACGCG
ATATGCGAGAAAACCCCCATAGTGGCTATCGTGCCGTCCACGTCTGGCTGCGACTGCCGGCCGGCCGCGTGGAGGTCCAG
ATCCGAACAGTGCCTCAGAGCGAATGGGCGAACACCTACGAGCGACTGGGCGACCGGTACGGTCGAGGTATCCGCTACGG
CGCGTCGGCAGAGGACGATGAAGCGCGCGAGCTGGTCGAGAGAATGCACAAGATGTCCGCGGCGTTAGCAAGTAGTGAGG
AGTCCACGGTCGAGTTGGCCCAACTCGAAGACGAGCTCCATCAGCTAAAGGAAAGGCTGGCGGAGATGCGGCCGCTCGCG
AGGGCGTCGGGGCCGATTAATCACCGGAGAGTGTCGAAGGCGCTGGATAGGACCTTCCGCAAGGCCGAGGAGACAAGGAC
GCGATTGAACCAGCACCGGGATTCGTACTTGCAAATGCTCCGCGAGATGCGCAGTATGCTCGATACAGACGGGAGTTGA
Antitoxin
Download Length: 72 a.a. Molecular weight: 8339.51 Da Isoelectric Point: 7.3206
>AT10006 YP_009304222.1 NC_031266:26012-26227 [Mycobacterium phage Phrann]
MPGFLIEFNRRTRERRVREFVDHSEAMHVRLQLEAAREDQDVEIAALISPSLETLKRTHSRYFTGKELAAI
MPGFLIEFNRRTRERRVREFVDHSEAMHVRLQLEAAREDQDVEIAALISPSLETLKRTHSRYFTGKELAAI
Download Length: 216 bp
>AT10006 NC_031266:26012-26227 [Mycobacterium phage Phrann]
ATGCCAGGTTTCTTGATCGAGTTCAATCGCCGTACCCGCGAGCGTCGGGTGCGGGAGTTTGTCGACCACAGCGAGGCCAT
GCACGTCAGGCTCCAGCTTGAAGCCGCACGCGAAGACCAGGACGTTGAAATCGCAGCGCTGATCAGTCCATCCCTCGAGA
CCCTGAAGCGTACTCATTCGCGGTACTTCACCGGCAAGGAACTCGCGGCGATCTGA
ATGCCAGGTTTCTTGATCGAGTTCAATCGCCGTACCCGCGAGCGTCGGGTGCGGGAGTTTGTCGACCACAGCGAGGCCAT
GCACGTCAGGCTCCAGCTTGAAGCCGCACGCGAAGACCAGGACGTTGAAATCGCAGCGCTGATCAGTCCATCCCTCGAGA
CCCTGAAGCGTACTCATTCGCGGTACTTCACCGGCAAGGAACTCGCGGCGATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Tatsuaki Kurata et al. (2021) RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis. Molecular Cell 81(15):3160-3170.e9. [PubMed:34174184]
(2) Steffi Jimmy et al. (2020) A widespread toxin-antitoxin system exploiting growth control via alarmone signaling. Proceedings of The National Academy of Sciences of The United States of America 117(19):10500-10510. [PubMed:32345719]