Detailed information of TA system TA100054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2552485..2553098
• Replicon: chromosome
• Accession: NZ_CP027731
• Organism: Pseudomonas sp. R2-60-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J90_RS11735
• Protein ID: WP_116665433.1
• Coordinates: 2552847..2553098 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J90_RS11730
• Protein ID: WP_124419833.1
• Coordinates: 2552485..2552850 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J90_RS26970	2548451..2549755	+	1305	WP_177413398.1	hypothetical protein	-
C4J90_RS11720	2549918..2551273	+	1356	WP_124419831.1	MATE family efflux transporter	-
C4J90_RS11725	2551313..2552518	+	1206	WP_124419832.1	MFS transporter	-
C4J90_RS11730	2552485..2552850	-	366	WP_124419833.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J90_RS11735	2552847..2553098	-	252	WP_116665433.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9508.07 Da
• Isoelectric Point: 10.0459

>T100054 WP_116665433.1 NZ_CP027731:c2553098-2552847 [Pseudomonas sp. R2-60-08W]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100054 NZ_CP027731:c2553098-2552847 [Pseudomonas sp. R2-60-08W]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAACTCGTAACCCTGTT
ACGGCGACTGGGTTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAGTGCAATGATCA
CCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13220.07 Da
• Isoelectric Point: 6.7400

>AT100054 WP_124419833.1 NZ_CP027731:c2552850-2552485 [Pseudomonas sp. R2-60-08W]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLHHHFPQPCAAIG

Nucleotide

• Length: 366 bp

>AT100054 NZ_CP027731:c2552850-2552485 [Pseudomonas sp. R2-60-08W]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTGGTGAGTTACGAAGGCAAGACCGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCGGTGGACG
ATTACCTCGCCACCTGCCAGTCGCTCGGGCAAACTCCCGAAAAGCCATGCAAGGGCTCGTTCAACGTACGCGTCGGCCAT
GACCTGCATCTGGCGGCAGCGTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACACGCCAGGCGCTGGATGA
GTTTCTACACCATCATTTCCCGCAGCCTTGCGCGGCGATTGGCTGA