Detailed information of TA system TA100052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1813044..1813648
• Replicon: chromosome
• Accession: NZ_CP027731
• Organism: Pseudomonas sp. R2-60-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S2UAI9
• Locus tag: C4J90_RS08295
• Protein ID: WP_065901605.1
• Coordinates: 1813044..1813232 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J90_RS08300
• Protein ID: WP_048729935.1
• Coordinates: 1813244..1813648 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J90_RS08270	1809511..1810047	+	537	WP_124414238.1	DsbE family thiol:disulfide interchange protein	-
C4J90_RS08275	1810044..1810517	+	474	WP_124419591.1	cytochrome c-type biogenesis protein CcmH	-
C4J90_RS08280	1810510..1811694	+	1185	WP_124419592.1	c-type cytochrome biogenesis protein CcmI	-
C4J90_RS08285	1811710..1812105	+	396	WP_124361952.1	hypothetical protein	-
C4J90_RS08290	1812297..1812863	+	567	WP_124361954.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J90_RS08295	1813044..1813232	+	189	WP_065901605.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J90_RS08300	1813244..1813648	+	405	WP_048729935.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J90_RS08305	1813645..1814841	-	1197	WP_124361957.1	MFS transporter	-
C4J90_RS08310	1815066..1816070	-	1005	WP_124419593.1	sulfate ABC transporter substrate-binding protein	-
C4J90_RS08315	1816167..1816991	-	825	WP_124419594.1	ion transporter	-
C4J90_RS08320	1817022..1817897	-	876	WP_124419595.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7219.42 Da
• Isoelectric Point: 11.2000

>T100052 WP_065901605.1 NZ_CP027731:1813044-1813232 [Pseudomonas sp. R2-60-08W]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100052 NZ_CP027731:1813044-1813232 [Pseudomonas sp. R2-60-08W]
GTGCAAAGCAGGCTATTGATCAAAGAGCTGCAAGAGGCGGGTTGGGTGCTGGATCGTGTCACCGGCAGCCATCACATTTT
TACCCACCGCTATAACCCGTACACGATCCCGGTGCCTCATCCGAAGAAAGATCTTCCCTTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14703.69 Da
• Isoelectric Point: 4.6000

>AT100052 WP_048729935.1 NZ_CP027731:1813244-1813648 [Pseudomonas sp. R2-60-08W]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100052 NZ_CP027731:1813244-1813648 [Pseudomonas sp. R2-60-08W]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTTACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTATAACGCGGCCGTGGAGGTTGCGCACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGGAGTGATTCCGATGCCAACCTCAGTCGCCAATCATCACGCCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAGCTGGATATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAACGTGACCTTGCCCGGCTATGTGATCCAACGCAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCTTTGGAGAAGTTGGTGCGGT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A1S2UAI9