Detailed information of TA system TA100048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2624574..2625187
• Replicon: chromosome
• Accession: NZ_CP027730
• Organism: Pseudomonas sp. R3-52-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J91_RS12180
• Protein ID: WP_124362546.1
• Coordinates: 2624936..2625187 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J91_RS12175
• Protein ID: WP_124362545.1
• Coordinates: 2624574..2624939 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J91_RS12155	2620541..2621521	+	981	WP_124362542.1	hypothetical protein	-
C4J91_RS12160	2621518..2621844	+	327	WP_177412825.1	hypothetical protein	-
C4J91_RS12165	2622007..2623362	+	1356	WP_124362543.1	MATE family efflux transporter	-
C4J91_RS12170	2623402..2624607	+	1206	WP_124362544.1	MFS transporter	-
C4J91_RS12175	2624574..2624939	-	366	WP_124362545.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J91_RS12180	2624936..2625187	-	252	WP_124362546.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9520.13 Da
• Isoelectric Point: 10.0459

>T100048 WP_124362546.1 NZ_CP027730:c2625187-2624936 [Pseudomonas sp. R3-52-08]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYIQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100048 NZ_CP027730:c2625187-2624936 [Pseudomonas sp. R3-52-08]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAGCTCGTAACGCTGTT
GCGCCGACTGGGCTACATACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAGTGCAATGATCA
CCTTGCACAAGCCGCACCCTGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13208.01 Da
• Isoelectric Point: 6.7400

>AT100048 WP_124362545.1 NZ_CP027730:c2624939-2624574 [Pseudomonas sp. R3-52-08]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLHHHFPQPCAATG

Nucleotide

• Length: 366 bp

>AT100048 NZ_CP027730:c2624939-2624574 [Pseudomonas sp. R3-52-08]
GTGAACAACCAACTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTGGTGAGCTACGAAGGCAAGACTGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCGGTGGACG
ATTACCTCGCCACCTGCCAGTCGCTCGGGCAAACTCCTGAAAAGCCATGCAAGGGCTCGTTCAACGTACGCGTCGGCCAT
GACCTGCATCTGGCGGCAGCGTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACACGCCAGGCGTTGGATGA
GTTTCTACACCATCATTTCCCGCAGCCTTGCGCGGCGACCGGCTGA