Detailed information of TA system TA100045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1598306..1598819
• Replicon: chromosome
• Accession: NZ_CP027730
• Organism: Pseudomonas sp. R3-52-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J91_RS07295
• Protein ID: WP_124361707.1
• Coordinates: 1598306..1598590 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J91_RS07300
• Protein ID: WP_124361708.1
• Coordinates: 1598580..1598819 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J91_RS07275	1594492..1595433	+	942	WP_124361703.1	hydroxymethylglutaryl-CoA lyase	-
C4J91_RS07280	1595492..1596823	+	1332	WP_124361704.1	MFS transporter	-
C4J91_RS07285	1596824..1597312	-	489	WP_124361705.1	DMT family transporter	-
C4J91_RS07290	1597381..1598298	+	918	WP_124361706.1	LysR family transcriptional regulator	-
C4J91_RS07295	1598306..1598590	-	285	WP_124361707.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J91_RS07300	1598580..1598819	-	240	WP_124361708.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4J91_RS07305	1598920..1599354	+	435	WP_124361709.1	fosfomycin resistance glutathione transferase	-
C4J91_RS07310	1599384..1600025	+	642	WP_124361710.1	LysE family translocator	-
C4J91_RS07315	1600146..1600673	+	528	WP_124361711.1	DUF4142 domain-containing protein	-
C4J91_RS07320	1600728..1601153	-	426	WP_124361712.1	low affinity iron permease family protein	-
C4J91_RS07325	1601330..1602112	+	783	WP_124361713.1	GNAT family N-acetyltransferase	-
C4J91_RS07330	1602113..1602613	-	501	WP_124361714.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11014.73 Da
• Isoelectric Point: 10.1506

>T100045 WP_124361707.1 NZ_CP027730:c1598590-1598306 [Pseudomonas sp. R3-52-08]
MQVEWLRTALRNLDDEAAFIAEENPKAAYEFVQAILAGLEHIAQFPALGKTGRVPGTREWVVPRWPYIVPYRIREGRLQV
MRIFHTRRQPPTSW

Nucleotide

• Length: 285 bp

>T100045 NZ_CP027730:c1598590-1598306 [Pseudomonas sp. R3-52-08]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGAAACCTGGATGATGAAGCGGCCTTTATTGCTGAAGAAAACCCCAAAGC
CGCCTACGAGTTCGTCCAGGCGATTTTGGCCGGGCTTGAACACATTGCGCAATTTCCCGCGCTGGGAAAAACCGGGCGCG
TCCCAGGGACGCGTGAATGGGTGGTGCCCCGGTGGCCCTACATTGTCCCTTACCGTATTCGTGAAGGGCGACTACAGGTG
ATGCGAATTTTTCATACCCGCCGCCAACCACCGACCTCCTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8597.72 Da
• Isoelectric Point: 4.7571

>AT100045 WP_124361708.1 NZ_CP027730:c1598819-1598580 [Pseudomonas sp. R3-52-08]
MSVMSLRIPDDVADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALKEADAGDFATAAEVKAVADKWRNNAG

Nucleotide

• Length: 240 bp

>AT100045 NZ_CP027730:c1598819-1598580 [Pseudomonas sp. R3-52-08]
ATGTCTGTTATGTCCCTGCGGATACCGGACGATGTCGCCGATACGCTTGCCAGTCTGGCCAAAGCGACCGGTCGCAGCAA
ATCGTTCCTCGCTGTTGACGCGCTACGTGAATACTTGACGCGTGAAGCATGGCAAATCGAAGAAATTCAAAAGGCGCTGA
AAGAAGCTGATGCGGGGGATTTCGCGACGGCTGCCGAGGTAAAAGCCGTCGCTGACAAATGGCGCAACAATGCAGGTTGA