Detailed information of TA system TA100037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1855587..1856191
• Replicon: chromosome
• Accession: NZ_CP027729
• Organism: Pseudomonas sp. R3-18-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S2UAI9
• Locus tag: C4J92_RS08550
• Protein ID: WP_065901605.1
• Coordinates: 1855587..1855775 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J92_RS08555
• Protein ID: WP_124403546.1
• Coordinates: 1855787..1856191 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J92_RS08520	1851328..1851864	+	537	WP_124403543.1	DsbE family thiol:disulfide interchange protein	-
C4J92_RS08525	1851861..1852334	+	474	WP_124403544.1	cytochrome c-type biogenesis protein CcmH	-
C4J92_RS08530	1852327..1853510	+	1184	Protein_1648	c-type cytochrome biogenesis protein CcmI	-
C4J92_RS08535	1853526..1853921	+	396	WP_124403545.1	hypothetical protein	-
C4J92_RS08540	1854087..1854362	-	276	WP_124361953.1	DUF3077 domain-containing protein	-
C4J92_RS08545	1854840..1855406	+	567	WP_124361954.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J92_RS08550	1855587..1855775	+	189	WP_065901605.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J92_RS08555	1855787..1856191	+	405	WP_124403546.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J92_RS08560	1856188..1857384	-	1197	WP_124403547.1	MFS transporter	-
C4J92_RS08565	1857625..1858629	-	1005	WP_124403548.1	sulfate ABC transporter substrate-binding protein	-
C4J92_RS08570	1858726..1859550	-	825	WP_124361959.1	ion transporter	-
C4J92_RS08575	1859582..1860457	-	876	WP_124403549.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7219.42 Da
• Isoelectric Point: 11.2000

>T100037 WP_065901605.1 NZ_CP027729:1855587-1855775 [Pseudomonas sp. R3-18-08]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100037 NZ_CP027729:1855587-1855775 [Pseudomonas sp. R3-18-08]
GTGCAAAGCAGGCTATTGATCAAAGAGCTGCAAGAGGCGGGTTGGGTGCTGGATCGTGTCACCGGCAGCCATCATATTTT
CACCCACCGCTATAACCCGTACACGATTCCGGTGCCTCATCCGAAGAAAGATCTTCCCTTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14717.72 Da
• Isoelectric Point: 4.6000

>AT100037 WP_124403546.1 NZ_CP027729:1855787-1856191 [Pseudomonas sp. R3-18-08]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGVAIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100037 NZ_CP027729:1855787-1856191 [Pseudomonas sp. R3-18-08]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCCGGGGACAGTTTCGAAGAGGCGTACAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGTAGCGATTCCGATGCCAACCTCAGTCGCCAATCATCACGCCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAGCTGGATATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTATGTCATCCAACGCAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCTTTGGAGAAGTTGGTGCGGT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A1S2UAI9