Detailed information of TA system TA100035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 962129..962768
• Replicon: chromosome
• Accession: NZ_CP027729
• Organism: Pseudomonas sp. R3-18-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J92_RS04310
• Protein ID: WP_124369634.1
• Coordinates: 962129..962311 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J92_RS04315
• Protein ID: WP_124403066.1
• Coordinates: 962367..962768 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J92_RS04295	958018..960303	-	2286	WP_124403063.1	TonB-dependent siderophore receptor	-
C4J92_RS04300	960509..961189	+	681	WP_124403064.1	Fe2+-dependent dioxygenase	-
C4J92_RS04305	961191..961940	+	750	WP_124403065.1	sel1 repeat family protein	-
C4J92_RS04310	962129..962311	+	183	WP_124369634.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J92_RS04315	962367..962768	+	402	WP_124403066.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J92_RS04320	962844..964007	-	1164	WP_124361263.1	type III PLP-dependent enzyme	-
C4J92_RS04325	964482..965639	-	1158	WP_003171711.1	ABC transporter ATP-binding protein	-
C4J92_RS04330	965636..966289	-	654	WP_124361264.1	ABC transporter permease	-
C4J92_RS04335	966301..967197	-	897	WP_124403067.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6756.89 Da
• Isoelectric Point: 11.3464

>T100035 WP_124369634.1 NZ_CP027729:962129-962311 [Pseudomonas sp. R3-18-08]
MRSREVIELLVADGWFEVAVKGSHHQFKHPVKRGRVTVPHPKSELAKGTLHSIFRSAGLK

Nucleotide

• Length: 183 bp

>T100035 NZ_CP027729:962129-962311 [Pseudomonas sp. R3-18-08]
ATGAGAAGTCGAGAGGTGATTGAGCTACTGGTAGCGGACGGTTGGTTTGAGGTGGCGGTGAAGGGTAGTCATCACCAATT
CAAACATCCAGTGAAGCGAGGGCGGGTGACGGTCCCTCATCCCAAGTCAGAACTTGCAAAGGGCACTCTGCATAGTATTT
TCAGATCGGCAGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14481.34 Da
• Isoelectric Point: 4.3897

>AT100035 WP_124403066.1 NZ_CP027729:962367-962768 [Pseudomonas sp. R3-18-08]
MKFPVVVHKDADSDYSVTVPDIPGCFSAGGSFSEALDNVREAIALHLEGLVADQEALPQALEVDAHLDNPDFVGGVWAIV
DFDLTPYLGKSVRFNASLPEQLLLRIDERVLKDHRYASRSGFLATAALRELSA

Nucleotide

• Length: 402 bp

>AT100035 NZ_CP027729:962367-962768 [Pseudomonas sp. R3-18-08]
ATGAAATTTCCCGTGGTAGTCCATAAAGATGCTGATTCGGATTACAGCGTGACGGTTCCGGATATTCCGGGATGTTTCTC
GGCCGGAGGATCTTTTTCCGAAGCGCTGGACAATGTGCGTGAGGCGATAGCGCTGCATCTGGAAGGATTGGTTGCCGATC
AGGAAGCGCTCCCCCAGGCGCTGGAGGTCGATGCACATTTGGATAATCCGGATTTTGTCGGCGGTGTATGGGCGATAGTC
GACTTTGATCTCACGCCTTATCTGGGCAAGTCAGTCCGTTTCAACGCTTCCTTGCCGGAACAGTTGCTGTTGCGCATTGA
CGAGCGGGTGCTGAAGGATCATCGGTATGCGTCGCGGTCAGGTTTTCTGGCGACGGCAGCGCTGCGTGAGCTATCTGCCT
GA