Detailed information of TA system TA100030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2575805..2576418
• Replicon: chromosome
• Accession: NZ_CP027728
• Organism: Pseudomonas sp. R2-37-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J93_RS12030
• Protein ID: WP_124429419.1
• Coordinates: 2576167..2576418 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J93_RS12025
• Protein ID: WP_124362545.1
• Coordinates: 2575805..2576170 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J93_RS27210	2571808..2573013	+	1206	WP_177413531.1	hypothetical protein	-
C4J93_RS12015	2573238..2574593	+	1356	WP_124362543.1	MATE family efflux transporter	-
C4J93_RS12020	2574633..2575838	+	1206	WP_124429418.1	MFS transporter	-
C4J93_RS12025	2575805..2576170	-	366	WP_124362545.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J93_RS12030	2576167..2576418	-	252	WP_124429419.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.99 Da
• Isoelectric Point: 9.6591

>T100030 WP_124429419.1 NZ_CP027728:c2576418-2576167 [Pseudomonas sp. R2-37-08W]
VSKNEKLLAKLLNEHMAFTWSELVTLLSRLGYTQIEGAGSRVKFDIGDPNAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100030 NZ_CP027728:c2576418-2576167 [Pseudomonas sp. R2-37-08W]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCTGAACTCGTAACCCTGTT
AAGTCGACTGGGTTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGACATTGGCGACCCCAATGCAATGATCA
CCTTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13208.01 Da
• Isoelectric Point: 6.7400

>AT100030 WP_124362545.1 NZ_CP027728:c2576170-2575805 [Pseudomonas sp. R2-37-08W]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALDEFLHHHFPQPCAATG

Nucleotide

• Length: 366 bp

>AT100030 NZ_CP027728:c2576170-2575805 [Pseudomonas sp. R2-37-08W]
GTGAACAACCAGCTGAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTGGTGAGCTACGAAGGCAAAACCGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCGGTGGACG
ATTACCTCGCCACCTGCCAGTCGCTCGGGCAAACTCCTGAAAAGCCATGCAAGGGCTCGTTCAATGTACGCGTCGGCCAT
GACCTGCATCTGGCAGCAGCGTTGGCGGCGAACCGCAAGAAGGTGACGTTGAACGACCTGACACGCCAGGCGCTGGATGA
GTTTCTACACCATCATTTCCCGCAGCCTTGCGCGGCGACTGGCTGA