Detailed information of TA system TA100029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1916264..1916868
• Replicon: chromosome
• Accession: NZ_CP027728
• Organism: Pseudomonas sp. R2-37-08W

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G8C3H2
• Locus tag: C4J93_RS08945
• Protein ID: WP_057722727.1
• Coordinates: 1916264..1916452 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J93_RS08950
• Protein ID: WP_048729935.1
• Coordinates: 1916464..1916868 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J93_RS08920	1912642..1913178	+	537	WP_124429123.1	DsbE family thiol:disulfide interchange protein	-
C4J93_RS08925	1913175..1913648	+	474	WP_124429124.1	cytochrome c-type biogenesis protein CcmH	-
C4J93_RS08930	1913641..1914825	+	1185	WP_124429125.1	c-type cytochrome biogenesis protein CcmI	-
C4J93_RS08935	1914841..1915236	+	396	WP_124370154.1	hypothetical protein	-
C4J93_RS08940	1915520..1916086	+	567	WP_124429126.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J93_RS08945	1916264..1916452	+	189	WP_057722727.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J93_RS08950	1916464..1916868	+	405	WP_048729935.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J93_RS08955	1916865..1918061	-	1197	WP_124429127.1	MFS transporter	-
C4J93_RS08960	1918301..1919305	-	1005	WP_124429128.1	sulfate ABC transporter substrate-binding protein	-
C4J93_RS08965	1919402..1920226	-	825	WP_124429129.1	ion transporter	-
C4J93_RS08970	1920258..1921133	-	876	WP_124429130.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7204.45 Da
• Isoelectric Point: 11.2000

>T100029 WP_057722727.1 NZ_CP027728:1916264-1916452 [Pseudomonas sp. R2-37-08W]
VQSRLLIKELLEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100029 NZ_CP027728:1916264-1916452 [Pseudomonas sp. R2-37-08W]
GTGCAAAGCAGGCTATTGATCAAGGAATTGCTGGAAGCGGGTTGGGTGCTGGATCGCGTCACCGGCAGCCATCACATTTT
TACCCACCGCTATAACCCGTACACGATCCCGGTGCCTCATCCGAAGAAGGATCTTCCCTTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14703.69 Da
• Isoelectric Point: 4.6000

>AT100029 WP_048729935.1 NZ_CP027728:1916464-1916868 [Pseudomonas sp. R2-37-08W]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100029 NZ_CP027728:1916464-1916868 [Pseudomonas sp. R2-37-08W]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCGGGTGCTGT
CACCGCTGGGGACAGTTTTGAAGAGGCGTATAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCCG
AGGGCGGAGTGATTCCGATGCCAACCTCAGTCGCCAATCATCATGCCCATGAAGACTACGCCGGCATGGGCTGGGGCATG
TTGGAGCTGGATATCTCGCCCTATCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGCTATGTCATCCAACGCAT
CGACCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCGGACGCGGCGTTGGAGAAGTTGGTGCGGT
CTTAA

Structures

Toxin

• AlphaFold DB: A0A3G8C3H2