Detailed information of TA system TA100028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1621555..1622068
• Replicon: chromosome
• Accession: NZ_CP027728
• Organism: Pseudomonas sp. R2-37-08W

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J93_RS07465
• Protein ID: WP_124428986.1
• Coordinates: 1621555..1621839 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J93_RS07470
• Protein ID: WP_124428987.1
• Coordinates: 1621829..1622068 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J93_RS07440	1616788..1617936	+	1149	WP_124428983.1	L-threonine dehydrogenase	-
C4J93_RS07445	1618028..1618702	+	675	WP_124369937.1	NAD-dependent epimerase/dehydratase family protein	-
C4J93_RS07450	1618779..1619987	+	1209	WP_124430854.1	CoA transferase	-
C4J93_RS07455	1620074..1620562	-	489	WP_124428984.1	DMT family transporter	-
C4J93_RS07460	1620630..1621547	+	918	WP_124428985.1	LysR family transcriptional regulator	-
C4J93_RS07465	1621555..1621839	-	285	WP_124428986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J93_RS07470	1621829..1622068	-	240	WP_124428987.1	ribbon-helix-helix protein, CopG family	Antitoxin
C4J93_RS07475	1622169..1622603	+	435	WP_124428988.1	fosfomycin resistance glutathione transferase	-
C4J93_RS07480	1622633..1623274	+	642	WP_124428989.1	LysE family translocator	-
C4J93_RS07485	1623395..1623922	+	528	WP_057723086.1	DUF4142 domain-containing protein	-
C4J93_RS07490	1623993..1624418	-	426	WP_124428990.1	low affinity iron permease family protein	-
C4J93_RS07495	1624595..1625377	+	783	WP_124369941.1	GNAT family N-acetyltransferase	-
C4J93_RS07500	1625378..1625878	-	501	WP_124428991.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
C4J93_RS07505	1625978..1626310	-	333	WP_124406521.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11014.73 Da
• Isoelectric Point: 10.1506

>T100028 WP_124428986.1 NZ_CP027728:c1621839-1621555 [Pseudomonas sp. R2-37-08W]
MQVEWLRTALRNLDDEAAFIAEENPKAAYEFVQAILAGLEHLAQFPALGKTGRVPGTREWVVPRWPYIVPYRIREGRLQV
MRIFHTRRQPPTSW

Nucleotide

• Length: 285 bp

>T100028 NZ_CP027728:c1621839-1621555 [Pseudomonas sp. R2-37-08W]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGAAACCTGGATGATGAAGCGGCCTTTATTGCTGAAGAAAACCCCAAAGC
CGCCTACGAATTCGTCCAGGCGATTTTGGCCGGGCTTGAACACCTTGCGCAATTTCCCGCGCTGGGAAAAACCGGGCGCG
TCCCAGGGACGCGTGAATGGGTGGTGCCCCGGTGGCCCTACATTGTCCCTTACCGTATTCGTGAAGGGCGACTACAGGTG
ATGCGAATTTTTCATACCCGCCGCCAACCACCGACCTCCTGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8570.61 Da
• Isoelectric Point: 4.3867

>AT100028 WP_124428987.1 NZ_CP027728:c1622068-1621829 [Pseudomonas sp. R2-37-08W]
MSVMSLRIPDDVADTLASLAKATGRSKSFLAVDALREYLTREAWQIEEIQKALTEADAGDFATAAEVNAVAEKWRNNAG

Nucleotide

• Length: 240 bp

>AT100028 NZ_CP027728:c1622068-1621829 [Pseudomonas sp. R2-37-08W]
ATGTCTGTTATGTCCCTGCGGATACCGGACGATGTCGCTGATACGCTTGCCAGTCTCGCCAAAGCGACCGGTCGCAGCAA
ATCGTTCCTCGCTGTTGACGCGTTACGTGAATACCTGACGCGTGAAGCATGGCAAATCGAAGAGATTCAAAAGGCGCTGA
CGGAAGCTGACGCGGGTGATTTCGCGACGGCTGCCGAGGTAAATGCCGTCGCTGAAAAATGGCGCAACAATGCAGGTTGA