Detailed information of TA system TA100026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2461651..2462264
• Replicon: chromosome
• Accession: NZ_CP027727
• Organism: Pseudomonas sp. R5-89-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J94_RS11395
• Protein ID: WP_116665433.1
• Coordinates: 2462013..2462264 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J94_RS11390
• Protein ID: WP_124386244.1
• Coordinates: 2461651..2462016 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J94_RS27685	2457634..2458920	+	1287	WP_177413450.1	hypothetical protein	-
C4J94_RS11380	2459083..2460438	+	1356	WP_124386242.1	MATE family efflux transporter	-
C4J94_RS11385	2460478..2461650	+	1173	WP_124386243.1	MFS transporter	-
C4J94_RS11390	2461651..2462016	-	366	WP_124386244.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J94_RS11395	2462013..2462264	-	252	WP_116665433.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9508.07 Da
• Isoelectric Point: 10.0459

>T100026 WP_116665433.1 NZ_CP027727:c2462264-2462013 [Pseudomonas sp. R5-89-07]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100026 NZ_CP027727:c2462264-2462013 [Pseudomonas sp. R5-89-07]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCCGAACTCGTAACGCTGTT
GCGTCGACTGGGTTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGATCCAAGTGCAATGATCA
CCCTGCACAAGCCTCACCCCGGCAACGAGTTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13229.13 Da
• Isoelectric Point: 6.3193

>AT100026 WP_124386244.1 NZ_CP027727:c2462016-2461651 [Pseudomonas sp. R5-89-07]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFQEAVDDYLATCQSLGQMPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALDEFLQHHFPEPCAAIG

Nucleotide

• Length: 366 bp

>AT100026 NZ_CP027727:c2462016-2461651 [Pseudomonas sp. R5-89-07]
GTGAACAACCAACTGAAACACAAAGGCTACATCGGCTCTATCGAAGCCAGCCTCGAGGACAACTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCCCTCGTGAGCTACGAAGGCAAAACCGTCGCAGAGTTGGACGCGGCCTTCCAAGAGGCGGTGGACG
ACTACCTCGCCACCTGCCAATCGCTCGGGCAGATGCCTGAAAAGCCCTGCAAGGGTTCTTTCAACGTACGCGTCGGCCAT
GACCTGCATTTGGCGGCAGCGTTGGCGGCGACCCGCAAGAAAGTGACGTTGAACGACCTGACACGCCAGGCGCTGGATGA
GTTTCTACAGCATCATTTCCCAGAGCCCTGCGCGGCGATTGGCTGA