Detailed information of TA system TA100024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1913523..1914127
• Replicon: chromosome
• Accession: NZ_CP027727
• Organism: Pseudomonas sp. R5-89-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S2UAI9
• Locus tag: C4J94_RS08865
• Protein ID: WP_065901605.1
• Coordinates: 1913523..1913711 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J94_RS08870
• Protein ID: WP_124361956.1
• Coordinates: 1913723..1914127 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J94_RS08840	1909915..1910451	+	537	WP_124385808.1	DsbE family thiol:disulfide interchange protein	-
C4J94_RS08845	1910448..1910921	+	474	WP_124385809.1	cytochrome c-type biogenesis protein CcmH	-
C4J94_RS08850	1910914..1912098	+	1185	WP_124385810.1	c-type cytochrome biogenesis protein CcmI	-
C4J94_RS08855	1912114..1912509	+	396	WP_124385811.1	hypothetical protein	-
C4J94_RS08860	1912798..1913364	+	567	WP_124385812.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
C4J94_RS08865	1913523..1913711	+	189	WP_065901605.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J94_RS08870	1913723..1914127	+	405	WP_124361956.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J94_RS08875	1914124..1915320	-	1197	WP_124385813.1	MFS transporter	-
C4J94_RS08880	1915546..1916550	-	1005	WP_124385814.1	sulfate ABC transporter substrate-binding protein	-
C4J94_RS08885	1916647..1917471	-	825	WP_124385815.1	ion transporter	-
C4J94_RS08890	1917503..1918378	-	876	WP_124385816.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7219.42 Da
• Isoelectric Point: 11.2000

>T100024 WP_065901605.1 NZ_CP027727:1913523-1913711 [Pseudomonas sp. R5-89-07]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T100024 NZ_CP027727:1913523-1913711 [Pseudomonas sp. R5-89-07]
GTGCAAAGCAGGCTATTGATCAAGGAGTTGCAAGAGGCGGGTTGGGTGCTGGATCGGGTCACTGGCAGTCATCATATTTT
TACCCACCGCTATAACCCGTACACGATCCCGGTGCCTCATCCAAAGAAGGATCTTCCCTTGGGCACCGTCAGAAGCATCA
GGAAGCGCGCCGGGCTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14690.69 Da
• Isoelectric Point: 4.6000

>AT100024 WP_124361956.1 NZ_CP027727:1913723-1914127 [Pseudomonas sp. R5-89-07]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGVIPMPTSVATHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT100024 NZ_CP027727:1913723-1914127 [Pseudomonas sp. R5-89-07]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCAGGCGCGGT
CACCGCCGGGGACAGTTTCGAAGAGGCTTACAACGCGGCCGTGGAGGTTGCACACATCATGCTGCAGGAGATCGCCGCAG
AGGGCGGAGTGATTCCGATGCCAACCTCGGTCGCCACTCATCACGCACATGAAGACTACGCCGGCATGGGCTGGGGTATG
TTGGAGCTGGATATCTCGCCCTATCTGGGCAAAACCGAGAAGGTCAATGTGACTTTGCCCGGTTATGTCATTCAGCGCAT
CGATCGTTACGTGCGCGAGCATAAAGTCAAAAGCCGCTCGTCATTTCTGGCTGATGCGGCTCTGGAGAAGTTGGTGCGTT
CCTAA

Structures

Toxin

• AlphaFold DB: A0A1S2UAI9