Detailed information of TA system TA100018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2536172..2536773
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4U3I3Z1
• Locus tag: C4J95_RS11685
• Protein ID: WP_057722035.1
• Coordinates: 2536522..2536773 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J95_RS11680
• Protein ID: WP_065884693.1
• Coordinates: 2536172..2536525 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS27470	2531625..2533238	+	1614	WP_206431942.1	hypothetical protein	-
C4J95_RS11670	2533604..2534959	+	1356	WP_124358145.1	MATE family efflux transporter	-
C4J95_RS11675	2535000..2536193	+	1194	WP_124358146.1	MFS transporter	-
C4J95_RS11680	2536172..2536525	-	354	WP_065884693.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J95_RS11685	2536522..2536773	-	252	WP_057722035.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9490.04 Da
• Isoelectric Point: 10.0459

>T100018 WP_057722035.1 NZ_CP027726:c2536773-2536522 [Pseudomonas orientalis]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100018 NZ_CP027726:c2536773-2536522 [Pseudomonas orientalis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCCGAGCTCGTAACGTTGTT
GCGCCGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGACCCCAGTGCACTGATTA
CCCTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 12737.61 Da
• Isoelectric Point: 7.3241

>AT100018 WP_065884693.1 NZ_CP027726:c2536525-2536172 [Pseudomonas orientalis]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFKEAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCPAIG

Nucleotide

• Length: 354 bp

>AT100018 NZ_CP027726:c2536525-2536172 [Pseudomonas orientalis]
GTGAACAACCAGCTCAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGTCTGTTCGGCAAAAT
CCTGTTCATCAAGGCGTTGGTGAGCTACGAAGGCAAAACCGTCGCCGAGTTGGACGCGGCTTTCAAGGAGGCGGTGGACG
ATTACCTGGCAACCTGCCAAGCCCTCGGGCAAACGCCGGAAAAGCCTTGCAAGGGCTCCTTCAACGTACGCGTGGGCCAT
GATCTGCATTTGGCGGCAGCGCTGGCGGCGACCCGTAAGAAGGTGACGTTGAACGACCTGACCCGCCAGGCGCTGAATGA
GTTTTTACAACATCATTGCCCGGCGATTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A4U3I3Z1