Detailed information of TA system TA100017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 2246688..2247216
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C4J95_RS10320
• Protein ID: WP_124357928.1
• Coordinates: 2246926..2247216 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: C4J95_RS10315
• Protein ID: WP_124357927.1
• Coordinates: 2246688..2246933 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS10285	2241862..2242353	-	492	WP_164484894.1	PilL N-terminal domain-containing protein	-
C4J95_RS10290	2242505..2243146	+	642	WP_124357922.1	glycoside hydrolase family 25 protein	-
C4J95_RS10295	2243212..2243685	-	474	WP_124357923.1	DUF3577 domain-containing protein	-
C4J95_RS10300	2244029..2244748	-	720	WP_124357924.1	ABC transporter substrate-binding protein	-
C4J95_RS10305	2244825..2245217	-	393	WP_124357925.1	hypothetical protein	-
C4J95_RS10310	2245466..2246368	-	903	WP_124357926.1	hypothetical protein	-
C4J95_RS10315	2246688..2246933	+	246	WP_124357927.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
C4J95_RS10320	2246926..2247216	+	291	WP_124357928.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J95_RS10325	2247221..2248501	-	1281	WP_124357929.1	DNA cytosine methyltransferase	-
C4J95_RS10330	2248479..2248880	-	402	WP_124357930.1	single-stranded DNA-binding protein	-
C4J95_RS10335	2248877..2249335	-	459	WP_206431949.1	DUF3158 family protein	-
C4J95_RS10340	2249332..2250093	-	762	WP_124357932.1	TIGR03761 family integrating conjugative element protein	-
C4J95_RS10345	2250270..2251409	-	1140	WP_124357933.1	hypothetical protein	-
C4J95_RS10350	2251413..2251973	-	561	WP_124357934.1	DUF2857 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vipB/mglB / fpvA / pvdE / pvdF / pvdO / pvdM / pvdP	2206062..2377975	171913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (7-73)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11245.94 Da
• Isoelectric Point: 7.8953

>T100017 WP_124357928.1 NZ_CP027726:2246926-2247216 [Pseudomonas orientalis]
MAEYRLAPAAERDLEVIWAYTVQQWSVEQAHRYIDLLTTAFAALAQSPKTAQACDHIRAGYRCSRVERHMVYFRITEYGI
AIIRVLHDRMDALRYL

Nucleotide

• Length: 291 bp

>T100017 NZ_CP027726:2246926-2247216 [Pseudomonas orientalis]
ATGGCTGAGTATCGCCTTGCACCGGCTGCCGAGCGCGACCTGGAGGTGATCTGGGCCTATACCGTTCAACAGTGGAGCGT
GGAGCAAGCGCATCGCTACATCGACCTCCTGACTACCGCTTTTGCTGCTTTGGCGCAGTCGCCCAAGACCGCCCAAGCCT
GTGACCACATTCGGGCGGGTTATCGTTGCAGCCGCGTTGAGCGGCACATGGTTTACTTTCGCATCACCGAGTACGGGATC
GCGATCATTCGTGTCTTGCATGATCGGATGGATGCGCTGCGTTACCTGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9110.12 Da
• Isoelectric Point: 6.4688

>AT100017 WP_124357927.1 NZ_CP027726:2246688-2246933 [Pseudomonas orientalis]
MGTVRKTITVTDKQDGWIKAQIDAGHYTNDSEYIRDLIRREQERSAEVEAIRSALIAGESSGEPRAFDADVFKQRMSAKH
G

Nucleotide

• Length: 246 bp

>AT100017 NZ_CP027726:2246688-2246933 [Pseudomonas orientalis]
ATGGGAACCGTACGTAAAACCATCACTGTGACCGACAAGCAGGACGGCTGGATCAAGGCTCAGATCGATGCCGGTCACTA
CACCAATGACAGCGAATACATCCGCGACTTGATCCGCCGTGAGCAGGAGCGTAGTGCCGAAGTGGAGGCGATTCGTTCGG
CGTTGATCGCCGGTGAGTCCAGCGGCGAACCGCGAGCCTTCGACGCGGACGTCTTCAAGCAGAGGATGTCGGCCAAGCAT
GGCTGA