Detailed information of TA system TA100016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 2222606..2223216
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J95_RS10190
• Protein ID: WP_124357903.1
• Coordinates: 2222606..2222908 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J95_RS10195
• Protein ID: WP_124357904.1
• Coordinates: 2222905..2223216 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS10170	2218289..2218588	-	300	WP_124357899.1	hypothetical protein	-
C4J95_RS10175	2218877..2219134	-	258	WP_124357900.1	DUF1778 domain-containing protein	-
C4J95_RS10180	2219183..2219686	-	504	WP_124357901.1	DNA repair protein RadC	-
C4J95_RS10185	2220490..2222439	+	1950	WP_164484893.1	AAA family ATPase	-
C4J95_RS10190	2222606..2222908	-	303	WP_124357903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J95_RS10195	2222905..2223216	-	312	WP_124357904.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C4J95_RS10200	2223282..2226110	-	2829	WP_124357905.1	conjugative transfer ATPase	-
C4J95_RS10205	2226110..2226520	-	411	WP_124357906.1	TIGR03751 family conjugal transfer lipoprotein	-
C4J95_RS10210	2226501..2227883	-	1383	WP_124357907.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vipB/mglB / fpvA / pvdE / pvdF / pvdO / pvdM / pvdP	2206062..2377975	171913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-54)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11780.39 Da
• Isoelectric Point: 5.1955

>T100016 WP_124357903.1 NZ_CP027726:c2222908-2222606 [Pseudomonas orientalis]
VKLEWSPWALKDRDAIFDFIEQDNPRAAVNIDSRIAEQTQRLIQFPESGRIGRCLGTRELLIDRTAYIAAYRIYDDRVRI
LRVLHEAQSWPEDLPTLVER

Nucleotide

• Length: 303 bp

>T100016 NZ_CP027726:c2222908-2222606 [Pseudomonas orientalis]
GTGAAGCTGGAGTGGTCACCTTGGGCCCTGAAGGATCGCGATGCCATCTTCGATTTCATTGAGCAGGACAACCCGCGTGC
AGCTGTCAACATAGATAGCCGGATTGCTGAACAGACCCAGCGGCTGATTCAGTTCCCGGAAAGCGGCCGAATTGGCCGTT
GCTTGGGCACGCGCGAGTTGCTAATTGATCGCACAGCATATATCGCTGCGTATCGGATCTACGATGACCGTGTCCGAATC
CTGCGCGTATTGCATGAGGCGCAGAGTTGGCCGGAGGATTTACCGACATTGGTGGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11161.61 Da
• Isoelectric Point: 5.8561

>AT100016 WP_124357904.1 NZ_CP027726:c2223216-2222905 [Pseudomonas orientalis]
MASNALIQTRIDPDVKDRATAVLQNLGLTVSDAVRILLTRVANEGALPFGFATDPATYDAWFRAKVQEALDDPRPAIAAE
DVEAHFAKRRAKAQRKLSEGGEA

Nucleotide

• Length: 312 bp

>AT100016 NZ_CP027726:c2223216-2222905 [Pseudomonas orientalis]
ATGGCCAGCAACGCCCTGATCCAGACCCGCATCGACCCGGACGTGAAAGACCGTGCCACGGCGGTCTTGCAAAACCTGGG
GCTAACAGTATCCGACGCCGTGCGCATCCTGCTCACCCGCGTCGCCAATGAAGGCGCCCTGCCCTTTGGGTTTGCCACCG
ACCCCGCCACCTATGACGCCTGGTTCCGAGCCAAAGTGCAGGAAGCCTTGGACGACCCGCGCCCGGCGATTGCCGCCGAA
GATGTGGAAGCCCACTTCGCCAAGCGTCGTGCCAAAGCCCAGCGTAAGCTGTCGGAAGGTGGTGAGGCGTGA