Detailed information of TA system TA100014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 943472..944111
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J95_RS04120
• Protein ID: WP_124357016.1
• Coordinates: 943472..943654 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J95_RS04125
• Protein ID: WP_124357017.1
• Coordinates: 943710..944111 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS04105	939350..941638	-	2289	WP_124357013.1	TonB-dependent siderophore receptor	-
C4J95_RS04110	941843..942523	+	681	WP_124357014.1	Fe2+-dependent dioxygenase	-
C4J95_RS04115	942525..943274	+	750	WP_124357015.1	sel1 repeat family protein	-
C4J95_RS04120	943472..943654	+	183	WP_124357016.1	type II toxin-antitoxin system HicA family toxin	Toxin
C4J95_RS04125	943710..944111	+	402	WP_124357017.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J95_RS04130	944184..945347	-	1164	WP_003188470.1	type III PLP-dependent enzyme	-
C4J95_RS04135	945783..946940	-	1158	WP_057701635.1	ABC transporter ATP-binding protein	-
C4J95_RS04140	946937..947590	-	654	WP_057726112.1	ABC transporter permease	-
C4J95_RS04145	947603..948496	-	894	WP_124357018.1	glycine betaine ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6728.84 Da
• Isoelectric Point: 11.3464

>T100014 WP_124357016.1 NZ_CP027726:943472-943654 [Pseudomonas orientalis]
MRSREVIELLVADGWFEVAAKGSHHQFKHPVKRGRVTVPHPKSEIAKGTLHSIFRSAGLK

Nucleotide

• Length: 183 bp

>T100014 NZ_CP027726:943472-943654 [Pseudomonas orientalis]
ATGAGAAGTCGAGAGGTGATTGAGCTACTGGTTGCGGATGGTTGGTTTGAGGTGGCAGCGAAGGGAAGCCATCACCAATT
TAAACATCCGGTGAAGCGGGGGCGAGTGACGGTCCCTCATCCCAAGTCAGAAATTGCCAAGGGCACTCTGCATAGTATTT
TCAGATCAGCAGGCCTCAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14411.16 Da
• Isoelectric Point: 4.4720

>AT100014 WP_124357017.1 NZ_CP027726:943710-944111 [Pseudomonas orientalis]
MKFPVVVHKDADSDYSVTVPDVPGCFSAGGSFSEALDNVREALALHLEGLVADQEALPQALEVDAHLGNPDFAGGVWAIV
DFDLTPYLGKSVRFNASLPEQLLQRIDERVQKDHRYASRSGFLATAALRELSA

Nucleotide

• Length: 402 bp

>AT100014 NZ_CP027726:943710-944111 [Pseudomonas orientalis]
ATGAAGTTTCCCGTGGTAGTCCATAAAGATGCTGACTCGGATTACAGCGTAACGGTCCCGGACGTACCAGGATGTTTCTC
GGCCGGAGGATCTTTTTCCGAAGCGCTGGACAATGTCCGTGAGGCTCTCGCGCTACATTTGGAGGGGTTGGTGGCAGATC
AGGAAGCGCTTCCCCAGGCACTGGAGGTCGATGCCCATCTGGGTAATCCGGATTTTGCTGGGGGCGTATGGGCGATAGTC
GACTTTGATCTGACGCCTTATCTGGGCAAGTCAGTCCGATTCAATGCTTCCTTGCCCGAGCAGTTGCTGCAACGCATTGA
TGAGCGGGTGCAGAAGGATCATCGCTATGCGTCGAGGTCAGGTTTTCTGGCGACGGCGGCACTGCGTGAGCTGTCTGCAT
GA