Detailed information of TA system TA100013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 691944..692534
• Replicon: chromosome
• Accession: NZ_CP027726
• Organism: Pseudomonas orientalis strain R4-35-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C4J95_RS02925
• Protein ID: WP_124356831.1
• Coordinates: 692235..692534 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C4J95_RS02920
• Protein ID: WP_124356830.1
• Coordinates: 691944..692231 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J95_RS02900	688352..690412	-	2061	WP_124356826.1	TonB-dependent copper receptor	-
C4J95_RS02905	690505..690912	-	408	WP_124356827.1	DUF2946 domain-containing protein	-
C4J95_RS02910	690924..691406	-	483	WP_124356828.1	copper chaperone PCu(A)C	-
C4J95_RS02915	691454..691846	-	393	WP_124356829.1	DUF2946 domain-containing protein	-
C4J95_RS02920	691944..692231	-	288	WP_124356830.1	putative addiction module antidote protein	Antitoxin
C4J95_RS02925	692235..692534	-	300	WP_124356831.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4J95_RS02930	692821..693540	-	720	WP_124356832.1	cobalt-precorrin-6A reductase	-
C4J95_RS02935	693537..694742	-	1206	WP_124356833.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
C4J95_RS02940	694781..696127	+	1347	WP_124356834.1	precorrin-3B synthase	-
C4J95_RS02945	696120..696746	+	627	WP_124356835.1	precorrin-8X methylmutase	-
C4J95_RS02950	696746..697477	+	732	WP_124356836.1	precorrin-2 C(20)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	621840..733172	111332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (2-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11570.12 Da
• Isoelectric Point: 8.0369

>T100013 WP_124356831.1 NZ_CP027726:c692534-692235 [Pseudomonas orientalis]
MIHFKRSDEFQEWLYSLRSKPAHGRVLTRLDNARMGNFGDCENVGNGVSEMRIHYGPGYRVYFKRIGEVVYLLLIGGDKS
TQKRDIERAKEIADEFEKE

Nucleotide

• Length: 300 bp

>T100013 NZ_CP027726:c692534-692235 [Pseudomonas orientalis]
ATGATTCACTTCAAGAGATCTGATGAGTTTCAGGAATGGCTTTATTCGCTACGCAGTAAACCCGCCCATGGACGCGTGCT
GACAAGATTGGATAACGCAAGAATGGGCAATTTTGGAGACTGCGAAAACGTCGGAAATGGCGTATCCGAAATGCGCATCC
ACTATGGTCCTGGATACCGTGTGTACTTCAAGCGTATAGGTGAGGTGGTTTACCTTCTACTGATAGGCGGCGATAAATCA
ACGCAAAAACGCGACATCGAACGCGCTAAAGAAATTGCCGATGAATTTGAAAAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10349.77 Da
• Isoelectric Point: 4.5972

>AT100013 WP_124356830.1 NZ_CP027726:c692231-691944 [Pseudomonas orientalis]
MTQFKDFDIAEHLTTPEDMAEYLEASFEEDAGDGLLIRSALNNIARAQGMTQIARDAGLGRESLYKALSSTGNPEFATIM
KVMKALGLKLHATSI

Nucleotide

• Length: 288 bp

>AT100013 NZ_CP027726:c692231-691944 [Pseudomonas orientalis]
ATGACTCAATTTAAAGATTTCGATATAGCCGAGCATCTGACGACCCCTGAGGACATGGCGGAGTATCTCGAAGCCTCTTT
CGAGGAGGACGCCGGTGACGGATTACTGATTCGCTCAGCGCTCAACAATATCGCCCGCGCCCAAGGCATGACCCAAATAG
CCCGCGACGCCGGCCTCGGACGAGAAAGTCTGTACAAAGCCCTCTCCAGCACGGGCAATCCCGAATTCGCGACTATCATG
AAAGTCATGAAAGCTCTGGGCCTCAAGCTTCATGCCACATCGATCTGA