Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 2425111..2425712 Replicon chromosome
Accession NZ_CP027725
Organism Pseudomonas orientalis strain R2-66-08W

Toxin (Protein)


Gene name hicA Uniprot ID A0A4U3I3Z1
Locus tag C4J96_RS11050 Protein ID WP_057722035.1
Coordinates 2425461..2425712 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1B5E0X2
Locus tag C4J96_RS11045 Protein ID WP_065894293.1
Coordinates 2425111..2425464 (-) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J96_RS27410 2420567..2422180 + 1614 WP_206425505.1 hypothetical protein -
C4J96_RS11035 2422543..2423898 + 1356 WP_124358145.1 MATE family efflux transporter -
C4J96_RS11040 2423939..2425132 + 1194 WP_124426536.1 MFS transporter -
C4J96_RS11045 2425111..2425464 - 354 WP_065894293.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4J96_RS11050 2425461..2425712 - 252 WP_057722035.1 type II toxin-antitoxin system HicA family toxin Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-76)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9490.04 Da        Isoelectric Point: 10.0459

>T100009 WP_057722035.1 NZ_CP027725:c2425712-2425461 [Pseudomonas orientalis]
VSKNEKLLAKLLNEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Download         Length: 252 bp

>T100009 NZ_CP027725:c2425712-2425461 [Pseudomonas orientalis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAATGAGCACATGGCATTCACCTGGTCCGAGCTCGTAACGTTGTT
GCGCCGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGACCCCAGTGCACTGATTA
CCCTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAAATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 12765.63 Da        Isoelectric Point: 7.3242

>AT100009 WP_065894293.1 NZ_CP027725:c2425464-2425111 [Pseudomonas orientalis]
VNNQLKHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCPAIG

Download         Length: 354 bp

>AT100009 NZ_CP027725:c2425464-2425111 [Pseudomonas orientalis]
GTGAACAACCAGCTCAAACACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGTCTGTTCGGCAAAAT
CCTGTTCATCAAGGCGTTGGTGAGCTACGAAGGCAAAACCGTCGCCGAGTTGGACGCGGCCTTCAGAGAGGCGGTGGACG
ATTACCTGGCAACCTGCCAAGCCCTCGGGCAAACGCCGGAAAAGCCTTGCAAGGGCTCCTTCAACGTACGCGTGGGCCAT
GATCTGCATTTGGCGGCAGCGCTGGCGGCGACCCGTAAGAAGGTGACGTTGAACGACCTGACCCGCCAGGCGCTGAATGA
GTTTTTACAACATCATTGCCCGGCGATTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U3I3Z1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B5E0X2

References