Detailed information of TA system TA100003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2414711..2415312
• Replicon: chromosome
• Accession: NZ_CP027724
• Organism: Pseudomonas orientalis strain L1-3-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C4J97_RS11010
• Protein ID: WP_124423315.1
• Coordinates: 2415061..2415312 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C4J97_RS11005
• Protein ID: WP_124423314.1
• Coordinates: 2414711..2415064 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J97_RS27490	2410227..2411774	+	1548	WP_206425421.1	hypothetical protein	-
C4J97_RS10995	2412143..2413498	+	1356	WP_124423312.1	MATE family efflux transporter	-
C4J97_RS11000	2413539..2414732	+	1194	WP_124423313.1	MFS transporter	-
C4J97_RS11005	2414711..2415064	-	354	WP_124423314.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C4J97_RS11010	2415061..2415312	-	252	WP_124423315.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9491.03 Da
• Isoelectric Point: 9.6583

>T100003 WP_124423315.1 NZ_CP027724:c2415312-2415061 [Pseudomonas orientalis]
VSKNEKLLAKLLDEHMAFTWSELVTLLRRLGYTQIEGAGSRVKFDIGDPSALITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T100003 NZ_CP027724:c2415312-2415061 [Pseudomonas orientalis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCGATGAGCACATGGCCTTCACCTGGTCCGAGCTCGTAACGCTGTT
ACGCCGACTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGGGTCAAATTCGATATCGGCGACCCCAGTGCACTGATCA
CCCTGCACAAGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 12843.70 Da
• Isoelectric Point: 7.3236

>AT100003 WP_124423314.1 NZ_CP027724:c2415064-2414711 [Pseudomonas orientalis]
VNNQLKYKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELEAAFREAVDDYLATCHSLGQTPEKPCKGSFNVRVGH
DLHLAAALAANRKKVTLNDLTRQALNEFLQHHCPAIG

Nucleotide

• Length: 354 bp

>AT100003 NZ_CP027724:c2415064-2414711 [Pseudomonas orientalis]
GTGAACAACCAGCTGAAATACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAATTGCCTGTTCGGCAAAAT
CCTGTTCATCAAGGCACTGGTGAGCTACGAAGGCAAAACCGTCGCCGAGTTGGAGGCGGCCTTCAGAGAGGCGGTGGACG
ATTACCTCGCAACCTGCCATTCCCTGGGGCAAACGCCGGAAAAGCCTTGCAAGGGGTCCTTCAACGTACGCGTCGGCCAT
GACCTGCACCTGGCTGCGGCCTTGGCGGCGAACCGCAAGAAAGTGACGTTGAACGACCTGACCCGCCAGGCGCTGAATGA
GTTTTTACAACATCATTGTCCGGCGATTGGCTGA