MGE detailed information
MGE type: Conjugative plasmid MGE length: 145346 bp
Accession: NZ_CP034739 Location: 1..145346 Organism: Escherichia coli strain L65 Replicon: plasmid pL65-2
Accession: NZ_CP034739 Location: 1..145346 Organism: Escherichia coli strain L65 Replicon: plasmid pL65-2
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EM854_RS23165 () | 497..1858 | - | 1362 | WP_000170681.1 | DUF3560 domain-containing protein | - |
| EM854_RS23170 () | 1910..2140 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| EM854_RS24690 () | 2818..3146 | + | 329 | Protein_3 | pilus assembly protein | - |
| EM854_RS23190 () | 3134..3316 | - | 183 | WP_001027505.1 | hypothetical protein | - |
| EM854_RS23195 () | 3316..3777 | - | 462 | WP_042007484.1 | DUF1380 family protein | - |
| EM854_RS23200 () | 3777..4079 | - | 303 | WP_072256028.1 | antirestriction protein | - |
| EM854_RS23210 () | 4604..5575 | + | 972 | WP_000082154.1 | IS110 family transposase ISEc32 | Transposase |
| EM854_RS23215 () | 5628..6325 | - | 698 | Protein_8 | IS1 family transposase | - |
| EM854_RS23220 () | 6379..6711 | - | 333 | Protein_9 | hypothetical protein | - |
| EM854_RS24695 () | 6780..7118 | - | 339 | WP_001513511.1 | hypothetical protein | - |
| EM854_RS23235 () | 7930..8901 | - | 972 | WP_000817028.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| EM854_RS23240 () | 8901..10067 | - | 1167 | WP_001238646.1 | Sporulation initiation inhibitor protein Soj | VF |
| EM854_RS23245 () | 10367..11064 | - | 698 | Protein_13 | IS1 family transposase | - |
| EM854_RS23250 () | 11163..11294 | - | 132 | Protein_14 | phosphopyruvate hydratase | - |
| EM854_RS23255 () | 11292..11549 | + | 258 | Protein_15 | Tn3 family transposase | - |
| EM854_RS23260 () | 11783..12640 | - | 858 | WP_000968140.1 | Manganese transport system membrane protein MntB | ARG |
| EM854_RS23265 () | 12637..13494 | - | 858 | WP_000992806.1 | Manganese transport system membrane protein MntB | ARG |
| EM854_RS23270 () | 13491..14318 | - | 828 | WP_000983710.1 | Iron(3+)-hydroxamate import ATP-binding protein FhuC | VF |
| EM854_RS23275 () | 14318..15232 | - | 915 | WP_000949004.1 | Putative metal ABC transporter substrate-binding protein Hpf | VF |
| EM854_RS23285 () | 15588..16285 | + | 698 | Protein_20 | IS1 family transposase | - |
| EM854_RS23290 () | 16465..16978 | - | 514 | Protein_21 | DUF4113 domain-containing protein | - |
| EM854_RS24700 () | 17039..17143 | - | 105 | Protein_22 | AAA family ATPase | - |
| EM854_RS23300 () | 17145..17468 | - | 324 | Protein_23 | IS21 family transposase IS21 | Transposase |
| EM854_RS23305 () | 17717..18930 | + | 1214 | WP_085947772.1 | IS3 family transposase ISCfr6 | Transposase |
| EM854_RS23310 () | 19475..20452 | + | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| EM854_RS23315 () | 20737..21477 | - | 741 | WP_001066954.1 | tyrosine-type recombinase/integrase | - |
| EM854_RS23320 () | 21598..21786 | - | 189 | WP_065203495.1 | serine/threonine protein kinase | - |
| EM854_RS23325 () | 22160..23062 | - | 903 | WP_001312822.1 | hypothetical protein | VF |
| EM854_RS23330 () | 23131..24240 | - | 1110 | WP_000771475.1 | SDR family oxidoreductase | - |
| EM854_RS23340 () | 24673..25626 | - | 954 | WP_000280980.1 | Protease 7 | VF |
| EM854_RS23355 () | 26900..27058 | - | 159 | WP_001312823.1 | integrase core domain-containing protein | - |
| EM854_RS23360 () | 27242..28441 | + | 1200 | Protein_32 | IS3 family transposase IS629 | Transposase |
| EM854_RS23365 () | 28589..28914 | - | 326 | Protein_33 | IS3 family transposase ISLad1 | Transposase |
| EM854_RS23370 () | 29895..31082 | + | 1188 | WP_000928804.1 | efflux RND transporter periplasmic adaptor subunit | - |
| EM854_RS23375 () | 31079..33019 | + | 1941 | WP_000733250.1 | Macrolide export ATP-binding/permease protein MacB | VF |
| EM854_RS23380 () | 33023..34393 | + | 1371 | WP_001312828.1 | efflux transporter outer membrane subunit | - |
| EM854_RS23390 () | 34867..35061 | + | 195 | Protein_37 | IS200-IS605 family transposase IS200C | Transposase |
| EM854_RS23395 () | 35190..36131 | - | 942 | WP_000974762.1 | hypothetical protein | - |
| EM854_RS23405 () | 36366..37755 | - | 1390 | Protein_39 | ISNCY family transposase ISKpn21 | Transposase |
| EM854_RS23410 () | 38065..38214 | - | 150 | Protein_40 | IS3 family transposase IS2 | Transposase |
| EM854_RS23415 () | 38392..39585 | - | 1194 | WP_000450494.1 | GTP-binding protein | - |
| EM854_RS23425 () | 40808..42044 | - | 1237 | Protein_42 | IS3 family transposase IS2 | Transposase |
| EM854_RS23430 () | 42671..42964 | + | 294 | WP_000738422.1 | increased serum survival lipoprotein Iss | - |
| EM854_RS23435 () | 43022..43221 | - | 200 | Protein_44 | lysis system i-spanin subunit Rz | - |
| EM854_RS23445 () | 43775..44875 | + | 1101 | Protein_45 | IS481 family transposase ISKpn28 | Transposase |
| EM854_RS23455 () | 46111..47226 | + | 1116 | WP_001318220.1 | Elloramycin glycosyltransferase ElmGT | VF |
| EM854_RS23460 () | 47366..51025 | + | 3660 | WP_001111199.1 | Multidrug efflux ATP-binding/permease protein | VF |
| EM854_RS23465 () | 51129..52358 | + | 1230 | WP_000933675.1 | Enterochelin esterase | VF |
| EM854_RS23470 () | 52443..53399 | + | 957 | WP_064055648.1 | Ferri-bacillibactin esterase BesA | VF |
| EM854_RS23475 () | 53444..55621 | - | 2178 | WP_001222186.1 | Ferric enterobactin receptor | VF |
| EM854_RS23480 () | 55856..56553 | + | 698 | Protein_51 | IS1 family transposase | - |
| EM854_RS23485 () | 56564..56767 | - | 204 | WP_001259759.1 | colicin V family bacteriocin | - |
| EM854_RS23490 () | 56745..56981 | - | 237 | WP_014640552.1 | colicin V immunity protein | - |
| EM854_RS23495 () | 57445..57726 | - | 282 | WP_001105066.1 | hypothetical protein | - |
| EM854_RS23500 () | 57899..58066 | + | 168 | Protein_55 | colicin-B | - |
| EM854_RS23505 () | 58084..58611 | - | 528 | WP_000203272.1 | colicin B immunity protein | - |
| EM854_RS23515 () | 58855..59670 | + | 816 | WP_001312845.1 | lipid II-degrading bacteriocin colicin M | - |
| EM854_RS23520 () | 59720..60073 | - | 354 | WP_000864812.1 | colicin M immunity protein | - |
| EM854_RS23525 () | 60251..61042 | - | 792 | WP_000016493.1 | site-specific integrase | - |
| EM854_RS23530 () | 61039..61728 | - | 690 | WP_000796228.1 | hypothetical protein | - |
| EM854_RS23535 () | 61772..62122 | - | 351 | WP_000493379.1 | hypothetical protein | - |
| EM854_RS23540 () | 62654..66475 | - | 3822 | WP_064055658.1 | hypothetical protein | - |
| EM854_RS24585 () | 66719..66880 | + | 162 | WP_000251882.1 | hypothetical protein | - |
| EM854_RS23545 () | 66898..67245 | - | 348 | WP_000142450.1 | hypothetical protein | - |
| EM854_RS23555 () | 67547..68029 | - | 483 | WP_001311056.1 | hypothetical protein | - |
| EM854_RS23560 () | 68146..68994 | - | 849 | WP_023154360.1 | 3'-5' exonuclease | - |
| EM854_RS23565 () | 69040..69321 | - | 282 | WP_000969990.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EM854_RS23570 () | 69318..69395 | - | 78 | Protein_68 | toxin-antitoxin system protein | - |
| EM854_RS23575 () | 69428..71179 | - | 1752 | Protein_69 | Tn3 family transposase TnpA_TnAs1 | Transposase |
| EM854_RS23580 () | 71168..71575 | + | 408 | WP_001214976.1 | cysteine hydrolase | - |
| EM854_RS23585 () | 71713..72597 | + | 885 | WP_000058717.1 | EamA family transporter | - |
| EM854_RS23590 () | 72629..73828 | - | 1200 | WP_000804064.1 | Tetracycline resistance protein, class C | ARG |
| EM854_RS23595 () | 73934..74584 | + | 651 | WP_000164043.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| EM854_RS23600 () | 74616..74858 | - | 243 | Protein_74 | relaxase | - |
| EM854_RS23605 () | 74916..75632 | - | 717 | Protein_75 | Tn3 family transposase | - |
| EM854_RS23610 () | 75666..78671 | - | 3006 | WP_001143760.1 | Tn3 family transposase TnpA_Tn3 | Transposase |
| EM854_RS23615 () | 78835..79392 | + | 558 | WP_001235713.1 | Tn3 family transposase TnpR_Tn3 | Transposase |
| EM854_RS23620 () | 79575..80435 | + | 861 | WP_015058868.1 | Beta-lactamase TEM | ARG |
| EM854_RS23625 () | 80583..80930 | - | 348 | Protein_79 | IS6 family transposase IS15DII | Transposase |
| EM854_RS23630 () | 81177..81650 | - | 474 | WP_004201280.1 | Dihydrofolate reductase type 1 | ARG |
| EM854_RS23635 () | 81806..82810 | + | 1005 | Protein_81 | IS91 family transposase ISAzo26 | Transposase |
| EM854_RS23640 () | 82864..83568 | - | 705 | WP_086525284.1 | IS6 family transposase IS26 | Transposase |
| EM854_RS23645 () | 83635..84251 | - | 617 | Protein_83 | transposase | - |
| EM854_RS23650 () | 84646..85302 | + | 657 | WP_001516695.1 | Pentapeptide repeat protein | ARG |
| EM854_RS23655 () | 85621..86049 | - | 429 | Protein_85 | Tn3 family transposase TnpR_ISMex22 | Transposase |
| EM854_RS23660 () | 86082..87473 | - | 1392 | WP_001493761.1 | ISKra4 family transposase ISKpn19 | Transposase |
| EM854_RS23665 () | 87510..88082 | - | 573 | WP_001493762.1 | Tn3 family transposase TnpR_ISPa40 | Transposase |
| EM854_RS23670 () | 88219..88809 | - | 591 | WP_012477564.1 | plasmid pRiA4b ORF-3 family protein | - |
| EM854_RS23675 () | 88927..89169 | + | 243 | WP_127528489.1 | relaxase | - |
| EM854_RS23680 () | 89201..89851 | - | 651 | WP_159186494.1 | tetracycline resistance transcriptional repressor TetR(A) | - |
| EM854_RS23685 () | 89958..91157 | + | 1200 | WP_032153701.1 | Tetracycline resistance protein, class C | ARG |
| EM854_RS23690 () | 91124..91312 | - | 189 | Protein_92 | IS91 family transposase | - |
| EM854_RS23695 () | 91424..91729 | - | 306 | WP_001255015.1 | HTH-type transcriptional regulator CynR | VF |
| EM854_RS23700 () | 91757..92971 | - | 1215 | WP_058100717.1 | Bicyclomycin resistance protein | ARG |
| EM854_RS24705 () | 93188..93634 | - | 447 | Protein_95 | DUF3363 domain-containing protein | - |
| EM854_RS23710 () | 94103..95596 | - | 1494 | WP_001120888.1 | IS91 family transposase ISVsa3 | Transposase |
| EM854_RS23715 () | 95807..96031 | - | 225 | WP_000743213.1 | hypothetical protein | - |
| EM854_RS23720 () | 96028..96765 | - | 738 | WP_001366550.1 | Tn3 family transposase TnpR_TnEc1 | Transposase |
| EM854_RS24590 () | 97251..97391 | - | 141 | WP_001044210.1 | hypothetical protein | - |
| EM854_RS23730 () | 97397..98101 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| EM854_RS23735 () | 98224..98625 | - | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| EM854_RS23740 () | 98558..98815 | - | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| EM854_RS23745 () | 98908..99561 | - | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| EM854_RS23760 () | 100501..101358 | - | 858 | WP_000130940.1 | incFII family plasmid replication initiator RepA | - |
| EM854_RS23765 () | 101351..101425 | - | 75 | WP_001365705.1 | RepA leader peptide Tap | - |
| EM854_RS23775 () | 101651..101911 | - | 261 | WP_000083818.1 | replication regulatory protein RepA | - |
| EM854_RS23780 () | 102150..102723 | - | 574 | Protein_107 | DUF2726 domain-containing protein | - |
| EM854_RS23785 () | 102761..102970 | - | 210 | WP_001333231.1 | hemolysin expression modulator Hha | - |
| EM854_RS23790 () | 103016..103477 | - | 462 | WP_001233871.1 | thermonuclease family protein | - |
| EM854_RS23795 () | 103720..103932 | - | 213 | WP_012311405.1 | hypothetical protein | - |
| EM854_RS23800 () | 104070..104630 | - | 561 | WP_000139359.1 | fertility inhibition protein FinO | - |
| EM854_RS23805 () | 104685..105431 | - | 747 | WP_000205777.1 | conjugal transfer pilus acetylase TraX | - |
| EM854_RS23810 () | 105451..110721 | - | 5271 | WP_072769080.1 | Multifunctional conjugation protein TraI | Relaxase |
| EM854_RS23815 () | 110721..112910 | - | 2190 | WP_106667887.1 | type IV conjugative transfer system coupling protein TraD | - |
| EM854_RS23825 () | 113163..113894 | - | 732 | WP_000850422.1 | complement resistance protein TraT | - |
| EM854_RS23830 () | 113926..114429 | - | 504 | WP_000605510.1 | hypothetical protein | - |
| EM854_RS23835 () | 114444..117266 | - | 2823 | WP_001007026.1 | conjugal transfer mating pair stabilization protein TraG | - |
| EM854_RS23840 () | 117263..118636 | - | 1374 | WP_001137358.1 | conjugal transfer protein TraH | - |
| EM854_RS23845 () | 118636..118998 | - | 363 | WP_001443292.1 | P-type conjugative transfer protein TrbJ | - |
| EM854_RS23850 () | 118928..119473 | - | 546 | WP_000052618.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| EM854_RS23855 () | 119460..119744 | - | 285 | WP_000624110.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| EM854_RS23860 () | 119863..120210 | - | 348 | WP_001287893.1 | conjugal transfer protein TrbA | - |
| EM854_RS23865 () | 120226..120969 | - | 744 | WP_001030378.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| EM854_RS23870 () | 120962..121219 | - | 258 | WP_000864325.1 | conjugal transfer protein TrbE | - |
| EM854_RS23875 () | 121246..123054 | - | 1809 | WP_000821821.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| EM854_RS23880 () | 123051..123689 | - | 639 | WP_001104248.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| EM854_RS23885 () | 123717..124169 | - | 453 | WP_000069427.1 | hypothetical protein | - |
| EM854_RS23890 () | 124199..124660 | - | 462 | WP_000277845.1 | hypothetical protein | - |
| EM854_RS23895 () | 124686..125678 | - | 993 | WP_000830839.1 | conjugal transfer pilus assembly protein TraU | - |
| EM854_RS23900 () | 125675..126307 | - | 633 | WP_001203745.1 | type-F conjugative transfer system protein TraW | - |
| EM854_RS23905 () | 126304..126690 | - | 387 | WP_000214084.1 | type-F conjugative transfer system protein TrbI | - |
| EM854_RS23910 () | 126687..129314 | - | 2628 | WP_001064268.1 | type IV secretion system protein TraC | - |
| EM854_RS23915 () | 129474..129695 | - | 222 | WP_001278689.1 | conjugal transfer protein TraR | - |
| EM854_RS23920 () | 129830..130345 | - | 516 | WP_000809906.1 | type IV conjugative transfer system lipoprotein TraV | - |
| EM854_RS23925 () | 130342..130593 | - | 252 | WP_001038341.1 | conjugal transfer protein TrbG | - |
| EM854_RS23930 () | 130590..130907 | - | 318 | WP_001057292.1 | conjugal transfer protein TrbD | - |
| EM854_RS23935 () | 130904..131476 | - | 573 | WP_000002780.1 | conjugal transfer protein TraP | - |
| EM854_RS23940 () | 131466..132893 | - | 1428 | WP_000146685.1 | conjugal transfer protein TraB | - |
| EM854_RS23945 () | 132893..133597 | - | 705 | WP_072095570.1 | type-F conjugative transfer system secretin TraK | - |
| EM854_RS23950 () | 133608..134174 | - | 567 | WP_000399792.1 | type IV conjugative transfer system protein TraE | - |
| EM854_RS23955 () | 134196..134507 | - | 312 | WP_000012106.1 | type IV conjugative transfer system protein TraL | - |
| EM854_RS23960 () | 134522..134881 | - | 360 | WP_000340272.1 | type IV conjugative transfer system pilin TraA | - |
| EM854_RS23965 () | 134915..135142 | - | 228 | WP_001254386.1 | conjugal transfer relaxosome protein TraY | - |
| EM854_RS23970 () | 135236..135922 | - | 687 | WP_000332484.1 | PAS domain-containing protein | - |
| EM854_RS23975 () | 136113..136496 | - | 384 | WP_000124981.1 | relaxosome protein TraM | - |
| EM854_RS23980 () | 136773..137420 | + | 648 | WP_000614936.1 | hypothetical protein | VF |
| EM854_RS23985 () | 137717..138538 | - | 822 | WP_001234469.1 | DUF945 domain-containing protein | - |
| EM854_RS23990 () | 138660..138947 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| EM854_RS24710 () | 138972..139178 | - | 207 | WP_000547939.1 | hypothetical protein | - |
| EM854_RS24010 () | 139869..140027 | - | 159 | WP_001312861.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| - () | 140071..140295 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - () | 140071..140295 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - () | 140071..140295 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| - () | 140071..140295 | - | 225 | NuclAT_0 | - | Antitoxin Detail |
| EM854_RS24595 () | 140107..140295 | + | 189 | WP_001299721.1 | hypothetical protein | - |
| EM854_RS24015 () | 140307..141026 | - | 720 | WP_001276228.1 | plasmid SOS inhibition protein A | - |
| EM854_RS24020 () | 141023..141457 | - | 435 | WP_000845895.1 | conjugation system SOS inhibitor PsiB | - |
| EM854_RS24025 () | 141512..143470 | - | 1959 | WP_001145469.1 | ParB/RepB/Spo0J family partition protein | - |
| EM854_RS24030 () | 143529..143762 | - | 234 | WP_000006004.1 | DUF905 domain-containing protein | - |
| EM854_RS24035 () | 143818..144345 | - | 528 | WP_000290793.1 | single-stranded DNA-binding protein | - |
| EM854_RS24055 () | 144818..145084 | - | 267 | WP_072254135.1 | hypothetical protein | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|