SecReT4

This Tool Annotates and Locates T4SS components in The DNA Sequence

Input Your DNA Sequence

To Paste a Sequence (FASTA format)

Example: example.fas

To Select a Completely Sequenced Bacterial Genome

Example: Plasmid F [NC_002483]

To Enter a Refseq Accession Number

Example: NC_002483

To Upload Your Sequence

Upload a FASTA-format File

You should:

Upload a Fasta File (Required) Example: NC_002483.fas
Upload a Ptt File (Optional) Example: NC_002483.ptt

Upload a GenBank-format File

You should:

Upload a GenBank File (Required) Example: NC_002483.gbk

Set Parameters

Blastp E-value <

Blastp Identity > (0~100) %

Maximum distance to co-localize two genes: (0~100000) bp **

* Coding sequence prediction will be skipped for annotated sequence.
** Identified T4SS genes within this distance will be clustered.

Please click "Run" after the required file has been uploaded.