| Effector Protein ID: 15611562 |
 T4SS name [ID] | Cag [38] |
| Gene symbol | cagA |
| Locus tag | jhp0495 |
| Strain | Helicobacter pylori J99 |
| Replicon | chromosome [GenBank: NC_000921] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 543605..547108 [+] |
| Product | cag island protein, cytotoxicity associated immunodominant antigen |
| Effector type | Protein |
| Descripton | CagA |
| T4CP requirement | T4CP Depedent |
| Target/Effection | CagA can cause gastritis and peptic ulcer, Injected CagA can act as a ‘master key’ that evolved the ability to highjack multiple host cell signalling cascades, which include the induction of membrane dynamics, actin-cytoskeletal rearrangements and the disruption of cell-to-cell junctions as well as proliferative, pro-inflammatory and anti-apoptotic nuclear responses (Backert et al, 2010). |
| UniProt ID | Q9ZLT1 |
| KEGG ID | hpj:jhp0495 |
| PDB ID | NA |
| Domain hit(s) vs Pfam | CagA [PF03507], Evalue: 5.8e-96, Aligned region: 868..1039 |
| Protein Sequence: 1167 a.a. [Download] |
| >gi|15611562|ref|NP_223213.1| cag island protein, cytotoxicity associated immunodominant antigen [Helicobacter pylori J99] MTNEAINQQPQTEAAFNPQQFINNLQVAFIKVDNVVASFDPNQKPIVDKNDRDNRQAFEKISQLREEFAN KAIKNPTKKNQYFSSFISKSNDLIDKDNLIDTGSSIKSFQKFGTQRYQIFMNWVSHQNDPSKINTQKIRG FMENIIQPPISDDKEKAEFLRSAKQAFAGIIIGNQIRSDQKFMGVFDESLKERQEAEKNGEPNGDPTGGD WLDIFLSFVFNKKQSSDLKETLNQEPVPHVQPDVATTTTDIQSLPPEARDLLDERGNFSKFTLGDMNMLD VEGVADIDPNYKFNQLLIHNNALSSVLMGSHNGIEPEKVSLLYGNNGGPEARHDWNATVGYKNQRGDNVA TLINVHMKNGSGLVIAGGEKGINNPSFYLYKEDQLTGSQRALSQEEIQNKVDFMEFLAQNNAKLDNLSKK EKEKFQNEIEDFQKDSKAYLDALGNDHIAFVSKKDKKHLALVAEFGNGELSYTLKDYGKKADKALDREAK TTLQGSLKHDGVMFVDYSNFKYTNASKSPDKGVGATNGVSHLEAGFSKVAVFNLPNLNNLAITSVVRQDL EDKLIAKGLSPQEANKLVKDFLSSNKELVGKALNFNKAVAEAKNTGNYDEVKQAQKDLEKSLKKRERLEK DVAKNLESKSGNKNKMEAKSQANSQKDEIFALINKEANRDARAIAYAQNLKGIKRELSDKLENINKDLKD FSKSFDEFKNGKNKDFSKAEETLKALKGSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSD LENSIKDVIINQKITDKVDNLNQAVSVAKATGDFSGVEQALADLKNFSKEQLAQQAQKNEDFNTGKNSAL YQSVKNGVNGTLVGNGLSKAEATTLSKNFSDIKKELNAKLGNFNNNNNNGLENSTEPIYTQVAKKVKAKI DRLDQIASGLGDVGQAASFLLKRHDKVDDLSKVGLSANHEPIYATIDDLGGPFPLKRHDKVDDLSKVGLS REQKLTQKIDNLNQAVSEAKASHFDNLDQMIDKLKDSTKKNVVNLYVESAKKVPTSLSAKLDNYATNSHT RINSNVKNGTINEKATGMLTQKNSEWLKLVNDKIVAHNVGSAPLSAYDKIGFNQKNMKDYSDSFKFSTRL SNAVKDIKSGFVQFLTNIFSMGSYSLMKASVEHGVKNTNTKGGFQKS |
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| Nucleotide Sequence: 3504 bp [Download] |
| >gi|15611071|ref|NC_000921.1|:543605-547108 Helicobacter pylori J99 chromosome, complete genome ATGACTAACGAAGCCATTAACCAACAACCACAAACCGAAGCGGCTTTTAACCCGCAGCAATTTATCAATA ATCTTCAAGTGGCTTTTATTAAAGTTGATAATGTTGTCGCTTCATTTGATCCTAATCAAAAACCAATCGT TGATAAGAATGATAGGGATAATAGGCAAGCTTTTGAGAAAATCTCGCAGCTAAGGGAGGAATTCGCTAAT AAAGCGATCAAAAATCCTACCAAAAAGAATCAGTATTTTTCAAGCTTTATCAGTAAGAGCAATGATTTAA TCGACAAAGACAATCTCATTGATACAGGTTCTTCCATAAAGAGCTTTCAGAAATTTGGGACTCAGCGTTA CCAAATTTTTATGAATTGGGTGTCCCATCAAAACGATCCGTCTAAAATCAACACCCAAAAAATCCGAGGT TTTATGGAAAATATCATACAACCCCCTATCTCTGATGATAAAGAGAAAGCGGAGTTTTTGAGGTCTGCCA AACAAGCTTTTGCAGGAATTATCATAGGAAACCAAATCCGATCGGATCAAAAATTCATGGGCGTGTTTGA TGAATCTTTGAAAGAGAGGCAAGAAGCAGAAAAAAATGGAGAGCCTAATGGAGATCCTACTGGTGGGGAT TGGCTTGATATTTTTTTATCATTTGTGTTTAACAAAAAACAATCTTCCGATCTCAAAGAAACGCTCAATC AAGAACCAGTTCCTCATGTCCAACCAGATGTAGCCACTACCACCACTGACATACAAAGCTTACCGCCTGA AGCTAGGGATTTGCTTGATGAAAGGGGTAATTTTTCTAAATTCACTCTTGGCGATATGAACATGTTAGAT GTTGAGGGAGTCGCTGACATTGATCCTAATTACAAGTTCAACCAATTATTGATCCACAATAACGCTCTGT CTTCTGTGTTAATGGGGAGTCATAATGGCATAGAACCTGAAAAAGTTTCATTGTTGTATGGAAACAATGG TGGTCCTGAAGCTAGGCATGATTGGAACGCCACCGTTGGTTATAAAAACCAACGAGGCGACAATGTGGCT ACACTCATTAATGTGCATATGAAAAATGGCAGTGGGTTAGTCATAGCAGGTGGTGAGAAAGGGATTAACA ACCCTAGTTTTTATCTCTACAAAGAAGACCAACTCACAGGCTCACAACGAGCATTGAGTCAAGAAGAGAT CCAAAACAAAGTGGATTTCATGGAATTTCTTGCACAAAATAATGCTAAATTAGACAACTTGAGCAAGAAA GAGAAAGAAAAATTCCAAAATGAGATTGAAGATTTTCAAAAAGACTCTAAGGCTTATTTAGACGCCCTAG GGAATGATCACATTGCTTTTGTTTCTAAAAAAGACAAAAAACATTTAGCTTTAGTTGCTGAGTTTGGTAA TGGGGAATTGAGCTACACTCTCAAAGATTATGGGAAAAAAGCAGATAAAGCTTTAGATAGGGAGGCAAAA ACCACTCTTCAAGGTAGCCTAAAACATGATGGCGTGATGTTTGTTGATTATTCTAATTTCAAATACACCA ACGCCTCCAAGAGTCCTGATAAGGGTGTGGGTGCTACGAATGGCGTTTCCCATTTAGAAGCAGGCTTTAG CAAGGTAGCTGTCTTTAATTTGCCTAATTTAAATAATCTCGCTATCACTAGTGTCGTAAGGCAGGATTTA GAGGATAAACTAATCGCTAAAGGATTGTCCCCACAAGAAGCTAATAAGCTTGTCAAAGATTTTTTGAGCA GCAACAAAGAATTGGTTGGAAAAGCTTTAAACTTCAATAAAGCTGTAGCTGAAGCTAAAAACACAGGCAA CTATGACGAGGTGAAACAAGCTCAGAAAGATCTTGAAAAATCTCTAAAGAAACGAGAGCGTTTGGAGAAA GATGTAGCGAAAAATTTGGAGAGCAAAAGCGGCAACAAAAATAAAATGGAAGCAAAATCTCAAGCTAACA GCCAAAAAGATGAGATTTTTGCGTTGATCAATAAAGAGGCTAATAGGGATGCAAGAGCAATCGCTTACGC TCAGAATCTTAAAGGCATCAAAAGGGAATTGTCTGATAAACTTGAAAATATCAACAAGGATTTGAAAGAC TTTAGTAAATCTTTTGATGAATTCAAAAATGGCAAAAATAAGGATTTCAGCAAGGCAGAAGAAACACTAA AAGCCCTTAAAGGCTCGGTGAAAGATTTAGGTATCAATCCAGAATGGATTTCAAAAGTTGAAAACCTTAA TGCAGCTTTGAATGAATTCAAAAATGGCAAAAATAAGGATTTCAGCAAGGTAACGCAAGCAAAAAGCGAC CTTGAAAATTCCATTAAAGATGTGATCATCAATCAAAAGATAACGGATAAAGTTGATAATCTCAATCAAG CGGTATCAGTGGCTAAAGCAACGGGTGATTTCAGTGGGGTAGAGCAAGCGTTAGCCGATCTCAAAAATTT CTCAAAGGAGCAATTGGCTCAACAAGCTCAAAAAAATGAAGATTTCAATACTGGAAAAAATTCTGCACTA TACCAATCCGTTAAGAATGGTGTAAACGGAACCCTAGTCGGTAATGGGTTATCTAAAGCAGAAGCCACAA CTCTTTCTAAAAACTTTTCGGACATCAAGAAAGAGTTGAATGCAAAACTTGGAAATTTCAATAACAATAA CAATAATGGACTCGAAAACAGCACAGAACCCATTTATACTCAAGTTGCTAAAAAGGTAAAAGCAAAAATT GACCGACTCGATCAAATAGCAAGTGGTTTGGGTGATGTAGGGCAAGCAGCGAGCTTCCTTTTGAAAAGGC ATGATAAAGTTGATGATCTCAGTAAGGTAGGGCTTTCAGCTAACCATGAACCCATTTACGCTACGATTGA TGATCTCGGCGGACCTTTCCCTTTGAAAAGGCATGATAAAGTTGATGATCTCAGTAAGGTAGGGCTTTCA AGGGAGCAAAAATTGACTCAGAAAATTGACAATCTCAACCAGGCGGTATCAGAAGCTAAAGCAAGTCATT TTGACAACCTAGATCAAATGATAGACAAGCTCAAAGATTCTACAAAAAAGAATGTTGTGAATCTATATGT TGAAAGTGCAAAAAAAGTGCCTACTAGTTTGTCAGCGAAATTGGACAATTACGCTACTAACAGCCACACA CGCATTAATAGCAATGTCAAAAATGGAACAATCAATGAAAAAGCGACCGGCATGCTAACGCAAAAAAATT CTGAGTGGCTCAAGCTCGTGAATGATAAGATAGTTGCGCATAATGTGGGAAGTGCTCCTTTGTCAGCGTA TGATAAAATTGGATTCAACCAAAAGAATATGAAAGATTATTCTGATTCGTTCAAGTTTTCCACCAGGTTG AGCAATGCCGTAAAAGACATTAAGTCTGGCTTTGTGCAATTTTTAACCAATATATTTTCTATGGGATCTT ACAGCTTGATGAAAGCAAGTGTGGAACATGGAGTCAAAAATACTAATACAAAAGGTGGTTTCCAAAAATC TTAA |
| Reference |
(1) Delgado-Rosado G; Dominguez-Bello MG; Massey SE (2011). Positive selection on a bacterial oncoprotein associated with gastric cancer. Gut Pathog. 3:18. [PudMed:22078307] 
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(2) Backert S; Churin Y; Meyer TF (2002). Helicobacter pylori type IV secretion, host cell signalling and vaccine development. Keio J Med. 51 Suppl 2:6-14. [PudMed:12528929]
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(3) Puls J; Fischer W; Haas R (2002). Activation of Helicobacter pylori CagA by tyrosine phosphorylation is essential for dephosphorylation of host cell proteins in gastric epithelial cells. Mol Microbiol. 43(4):961-9. [PudMed:11936078]
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(4) Moese S; Selbach M; Zimny-Arndt U; Jungblut PR; Meyer TF; Backert S (2001). Identification of a tyrosine-phosphorylated 35 kDa carboxy-terminal fragment (p35CagA) of the Helicobacter pylori CagA protein in phagocytic cells: processing or breakage. Proteomics. 1(4):618-29. [PudMed:11681214]
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| experimental literature |