| Effector Protein ID: 10954976 |
 T4SS name [ID] | VirB [6] |
| Gene symbol | tiorf156 |
| Locus tag | pTi-SAKURA_p158 |
| Strain | Agrobacterium tumefaciens plasmid pTi-SAKURA (NC_002147) |
| Replicon | plasmid pTi-SAKURA [GenBank: NC_002147] [Browse all T4SS(s) in this replicon] |
| Location [Strand] | 153202..154545 [+] |
| Product | type IV secretion system T-DNA border endonuclease VirD2 |
| Effector type | Protein |
| Descripton | VirD2 |
| T4CP requirement | T4CP Depedent |
| Target/Effection | VirD2 is an endonuclease that nicks the T-DNA region of Ti-/Ri-plasmids at border repeat sequences flanking T-DNA. During T-DNA processing, VirD2 covalently attaches to the 5' end of the resulting T-strand. VirD2 serves as a "pilot protein" to lead T-strands through the T4SS and into the host cell. Once in the host, VirD2 probably plays a major role in targeting T-strands to the nucleus |
| UniProt ID | Q9R6B9 |
| KEGG ID | NA |
| PDB ID | NA |
| Domain hit(s) vs Pfam | Relaxase [PF03432], Evalue: 6.9e-34, Aligned region: 16..213 |
| Protein Sequence: 447 a.a. [Download] |
| >gi|10954976|ref|NP_053396.1| hypothetical protein pTi-SAKURA_p158 [Agrobacterium tumefaciens] MPDRAQVIIRIVPGGGTKTLQQIINQLEYLSRKGRLELQRSARHLDIPLPPDQIHELARSWVQETGTYDE SQPDEERQQELTTHIIVSFPAGTSQVAAYAASREWAAEMFGSGAGGGRYNYLTAFHIDRDHPHLHVVVNR RELLGHGWLKISRRHPQLNYDALRIKMAEISLRHGIALDASRRAERGITERPITYAQYRRLEREQARQIR FEDADLEQSSPQGDHPEFSQPFDTSPFEASAGGPEDMPRPNNRQNESQVHLQEPAGVSNEAGVLVRVALE TERLAQPFVSETILADDIGSGSSRVAEGRVESANRTPDIPRAATEAATHTTHDRQRRAKRPHDDDGGPSG AKRVTLEGIAVGPQANAGEQDGSSGPLVRQAGTSRPSPPTATTRASTATDSLSATAHLQQRRGVPSKRPR EDDDGEPSERKRERDERSKDGREGNRR |
| - |
| Nucleotide Sequence: 1344 bp [Download] |
| >gi|10954820|ref|NC_002147.1|:153202-154545 Agrobacterium tumefaciens MAFF301001 plasmid pTi-SAKURA, complete sequence ATGCCCGATCGAGCTCAAGTTATCATTCGCATTGTGCCGGGAGGTGGCACCAAGACCCTTCAACAAATTA TCAATCAGTTGGAGTATCTATCCCGGAAGGGCAGGCTGGAGCTGCAGCGTTCAGCCCGACATCTCGATAT TCCCCTGCCACCGGATCAAATCCACGAACTTGCCCGAAGCTGGGTTCAAGAGACTGGAACTTATGACGAA AGTCAGCCAGACGAGGAAAGGCAACAGGAGTTGACCACCCATATTATTGTAAGCTTCCCCGCGGGTACAA GCCAGGTAGCGGCTTATGCGGCGAGCCGGGAGTGGGCAGCCGAGATGTTTGGGTCAGGCGCAGGGGGGGG CCGATACAACTATCTTACGGCCTTCCACATCGATCGCGACCACCCACATCTGCATGTCGTCGTCAATCGG CGCGAACTTTTAGGACACGGCTGGCTGAAGATATCTCGGCGCCATCCCCAACTGAATTACGACGCCCTGC GCATAAAGATGGCCGAGATTTCACTTCGTCATGGCATTGCCCTCGATGCGAGCCGACGAGCAGAACGTGG CATCACCGAGCGGCCGATCACTTATGCCCAATATCGGCGCCTTGAGCGGGAGCAGGCTCGCCAAATCCGT TTCGAAGACGCGGATTTGGAACAGTCGTCGCCGCAAGGAGATCATCCAGAATTCAGCCAACCTTTCGATA CATCCCCATTTGAAGCATCCGCGGGCGGACCGGAGGACATGCCTCGGCCCAACAATCGGCAGAATGAGTC GCAAGTTCATCTCCAGGAGCCAGCTGGTGTCAGCAACGAAGCCGGTGTCCTTGTGCGGGTTGCATTGGAG ACGGAGCGCCTTGCTCAACCATTCGTTTCCGAAACCATTCTCGCGGACGACATAGGGAGCGGCTCTTCGC GTGTTGCCGAGGGCCGTGTGGAGAGCGCAAACCGCACTCCCGATATTCCTCGCGCAGCAACTGAAGCTGC CACGCACACGACACACGACCGGCAGCGGCGTGCAAAGCGTCCTCATGATGACGACGGAGGGCCGAGTGGA GCAAAACGTGTGACATTGGAAGGCATCGCGGTTGGGCCCCAGGCGAACGCCGGCGAACAGGATGGCAGTA GTGGCCCCTTAGTACGGCAAGCTGGAACGTCTCGGCCATCTCCACCGACGGCCACGACGCGGGCCAGCAC CGCAACCGATTCATTGTCTGCTACAGCCCACCTCCAGCAACGGAGAGGTGTCCCTTCAAAGCGTCCGCGT GAAGATGATGATGGAGAACCGAGTGAACGCAAACGCGAGAGAGATGAGCGCAGCAAGGACGGGCGTGAGG GAAATAGGAGATAG |
| Reference |
| (1) Hattori Y; Iwata K; Suzuki K; Uraji M; Ohta N; Katoh A; Yoshida K (2001). Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA. Genes Genet Syst. 76(2):121-30. [PudMed:11434457] |