T4SS ID	616
Strain	Enterococcus faecalis plasmid pBEE99 (NC_013533)
Replicon	plasmid pBEE99 [Browse all T4SS(s) in this replicon]
Accession	NC_013533
Location	8127..34867
Name	Prg/Pcf
Function	conjugation
Classification	Type IVD; Type GP
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	PcfC	PcfH	PrgA	PrgB	PrgC	PrgD	PrgF	PrgH	PrgI
Number	1	1	0	1	1	1	1	1	1
Component	PrgJ	PrgK	PrgL
Number	1	1	1

The information of T4SS components from NC_013533
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pBEE99p04 (prgX)	3731..4714 [-], 984	270208276	PrgX
2	pBEE99p05 (prgR)	5534..5914 [+], 381	270208277	PrgR
3	pBEE99p06 (prgS)	5965..6186 [+], 222	270208278	PrgS
4	pBEE99p07	6539..6829 [+], 291	270208279	transposase
5	pBEE99p08	6865..7701 [+], 837	270208280	integrase
6	pBEE99p09 (prgB)	8127..12044 [+], 3918	270208281	PrgB	PrgB
7	pBEE99p10 (prgC)	12498..13355 [+], 858	270208282	PrgC	PrgC
8	pBEE99p11 (prgD)	13397..14296 [+], 900	270208283	PrgD	PrgD
9	pBEE99p12 (prgK)	14316..14750 [+], 435	270208284	PrgK
10	pBEE99p13 (prgF)	14797..15024 [+], 228	270208285	PrgF	PrgF
11	pBEE99p14	15038..15334 [+], 297	270208286	hypothetical protein
12	pBEE99p15 (prgH)	15349..16149 [+], 801	270208287	PrgH	PrgH
13	pBEE99p16 (prgI)	16151..16504 [+], 354	270208288	PrgI	PrgI
14	pBEE99p17 (prgJ)	16458..18848 [+], 2391	270208289	PrgJ	PrgJ
15	pBEE99p18 (prgK)	18860..21373 [+], 2514	270208290	PrgK	PrgK
16	pBEE99p19	21509..21838 [-], 330	270208291	hypothetical protein
17	pBEE99p20	22097..24031 [+], 1935	270208292	reverse transcriptase
18	pBEE99p21 (prgL)	24304..24930 [+], 627	270208293	PrgL	PrgL
19	pBEE99p22 (prgM)	24908..25162 [+], 255	270208294	PrgM
20	pBEE99p23 (pcfA)	25146..25754 [+], 609	270208295	PcfA
21	pBEE99p24	25867..26622 [+], 756	270208296	hypothetical protein
22	pBEE99p25 (pcfS)	26639..27097 [+], 459	270208297	PcfS
23	pBEE99p26	27143..27325 [+], 183	270208298	hypothetical protein
24	pBEE99p27 (pcfB)	27364..27852 [+], 489	270208299	PcfB
25	pBEE99p28 (pcfC)	27852..29681 [+], 1830	270208300	PcfC	PcfC
26	pBEE99p29 (pcfD)	29732..31891 [+], 2160	270208301	PcfD
27	pBEE99p30 (pcfE)	31924..32196 [+], 273	270208302	PcfE
28	pBEE99p31 (pcfF)	32442..32798 [+], 357	270208303	PcfF
29	pBEE99p32 (pcfG)	32799..34484 [+], 1686	270208304	PcfG
30	pBEE99p33 (pcfH)	34517..34867 [+], 351	270208305	PcfH	PcfH
31	pBEE99p34 (pcfJ)	35384..36727 [-], 1344	270208306	PcfJ
32	pBEE99p35	36727..37500 [-], 774	270208307	hypothetical protein
33	pBEE99p36	37605..38195 [-], 591	270208308	hypothetical protein
34	pBEE99p37 (pcfM)	38219..38410 [-], 192	270208309	PcfM
35	pBEE99p38 (pcfN)	38395..38580 [-], 186	270208310	PcfN
36	pBEE99p39 (pcfP)	38876..39076 [+], 201	270208311	PcfP
37	pBEE99p40 (pcfQ)	39178..39408 [-], 231	270208312	PcfQ

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	PcfF (270208303)	ND (interacts with PcfC T4CP)	PcfG	PcfF interacts with PcfC and PcfG, spatially positions relaxosome or transfer intermediate near the T4CP.	(1) PubMed: 19946141
2	PrgB (270208281)	ND	-	Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.	(2) PubMed: 25431047

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]

(2) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047]

(1) Coburn PS; Baghdayan AS; Craig N; Burroughs A; Tendolkar P; Miller K; Najar FZ; Roe BA; Shankar N (2010). A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation. Plasmid. 64(1):18-25. [PudMed:20307569]