T4SS ID616
StrainEnterococcus faecalis plasmid pBEE99 (NC_013533)
Repliconplasmid pBEE99 [Browse all T4SS(s) in this replicon]
ClassificationType IVD; Type GP
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_013533
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pBEE99p04 (prgX)3731..4714 [-], 984270208276PrgX 
2pBEE99p05 (prgR)5534..5914 [+], 381270208277PrgR 
3pBEE99p06 (prgS)5965..6186 [+], 222270208278PrgS 
4pBEE99p076539..6829 [+], 291270208279transposase 
5pBEE99p086865..7701 [+], 837270208280integrase 
6pBEE99p09 (prgB)8127..12044 [+], 3918270208281PrgB  PrgBaccesspry protein
7pBEE99p10 (prgC)12498..13355 [+], 858270208282PrgC  PrgC
8pBEE99p11 (prgD)13397..14296 [+], 900270208283PrgD  PrgD
9pBEE99p12 (prgK)14316..14750 [+], 435270208284PrgK 
10pBEE99p13 (prgF)14797..15024 [+], 228270208285PrgF  PrgF
11pBEE99p1415038..15334 [+], 297270208286hypothetical protein 
12pBEE99p15 (prgH)15349..16149 [+], 801270208287PrgH  PrgH
13pBEE99p16 (prgI)16151..16504 [+], 354270208288PrgI  PrgI
14pBEE99p17 (prgJ)16458..18848 [+], 2391270208289PrgJ  PrgJ
15pBEE99p18 (prgK)18860..21373 [+], 2514270208290PrgK  PrgK
16pBEE99p1921509..21838 [-], 330270208291hypothetical protein 
17pBEE99p2022097..24031 [+], 1935270208292reverse transcriptase 
18pBEE99p21 (prgL)24304..24930 [+], 627270208293PrgL  PrgL
19pBEE99p22 (prgM)24908..25162 [+], 255270208294PrgM 
20pBEE99p23 (pcfA)25146..25754 [+], 609270208295PcfA 
21pBEE99p2425867..26622 [+], 756270208296hypothetical protein 
22pBEE99p25 (pcfS)26639..27097 [+], 459270208297PcfS 
23pBEE99p2627143..27325 [+], 183270208298hypothetical protein 
24pBEE99p27 (pcfB)27364..27852 [+], 489270208299PcfB 
25pBEE99p28 (pcfC)27852..29681 [+], 1830270208300PcfC  PcfC
26pBEE99p29 (pcfD)29732..31891 [+], 2160270208301PcfD 
27pBEE99p30 (pcfE)31924..32196 [+], 273270208302PcfE 
28pBEE99p31 (pcfF)32442..32798 [+], 357270208303PcfF  accesspry protein
29pBEE99p32 (pcfG)32799..34484 [+], 1686270208304PcfG 
30pBEE99p33 (pcfH)34517..34867 [+], 351270208305PcfH  PcfH
31pBEE99p34 (pcfJ)35384..36727 [-], 1344270208306PcfJ 
32pBEE99p3536727..37500 [-], 774270208307hypothetical protein 
33pBEE99p3637605..38195 [-], 591270208308hypothetical protein 
34pBEE99p37 (pcfM)38219..38410 [-], 192270208309PcfM 
35pBEE99p38 (pcfN)38395..38580 [-], 186270208310PcfN 
36pBEE99p39 (pcfP)38876..39076 [+], 201270208311PcfP 
37pBEE99p40 (pcfQ)39178..39408 [-], 231270208312PcfQ 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1PcfF (270208303)ND (interacts with PcfC T4CP)PcfGPcfF interacts with PcfC and PcfG, spatially positions relaxosome or transfer intermediate near the T4CP.(1) PubMed: 19946141
2PrgB (270208281)ND-Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.(2) PubMed: 25431047
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Alvarez-Martinez CE; Christie PJ (2009). Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 73(4):775-808. [PudMed:19946141]
(2) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047] experimental
(1) Coburn PS; Baghdayan AS; Craig N; Burroughs A; Tendolkar P; Miller K; Najar FZ; Roe BA; Shankar N (2010). A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation. Plasmid. 64(1):18-25. [PudMed:20307569]