T4SS ID	614
Strain	Enterococcus faecalis V583
Replicon	plasmid pTEF2 [Browse all T4SS(s) in this replicon]
Accession	NC_004671
Location	7092..32433
Name	Prg/Pcf
Function	conjugation
Classification	Type IVD; Type GP
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	PcfC	PcfH	PrgA	PrgB	PrgC	PrgD	PrgF	PrgH	PrgI
Number	1	1	1	1	1	1	1	1	1
Component	PrgJ	PrgK	PrgL
Number	1	1	1

The information of T4SS components from NC_004671
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	EF_B0004 (traC-2)	2486..4075 [+], 1590	29377898	TraC protein
2	EF_B0005	4101..5042 [-], 942	29377899	Cro/CI family transcriptional regulator
3	EF_B00051	5255..5332 [+], 78	29377900	hypothetical protein
4	EF_B0007	5822..6202 [+], 381	29377901	pheromone-responsive regulatory protein R
5	EF_B0008	6776..6964 [+], 189	29377902	hypothetical protein
6	EF_B0009	6968..7081 [+], 114	29377903	hypothetical protein
7	EF_B0010 (prgA)	7092..9767 [+], 2676	29377904	surface exclusion protein PrgA	PrgA
8	EF_B0011 (prgB)	9960..13877 [+], 3918	29377905	aggregation substance PrgB	PrgB
9	EF_B0012 (prgC)	14323..15180 [+], 858	29377906	surface protein PrgC	PrgC
10	EF_B0013	15222..16121 [+], 900	29377907	hypothetical protein	PrgD
11	EF_B0014	16141..16575 [+], 435	29377908	hypothetical protein
12	EF_B0015	16622..16849 [+], 228	29377909	hypothetical protein	PrgF
13	EF_B0016	16863..17159 [+], 297	29377910	hypothetical protein
14	EF_B0017	17174..17974 [+], 801	29377911	hypothetical protein	PrgH
15	EF_B0018	17976..18329 [+], 354	29377912	hypothetical protein	PrgI
16	EF_B0019	18436..20673 [+], 2238	29377913	hypothetical protein	PrgJ
17	EF_B0020	20685..23300 [+], 2616	29377914	hypothetical protein	PrgK
18	EF_B0021	23324..23950 [+], 627	29377915	hypothetical protein	PrgL
19	EF_B0022	23928..24182 [+], 255	29377916	hypothetical protein
20	EF_B0023	24166..24774 [+], 609	29377917	hypothetical protein
21	EF_B0024	24931..25419 [+], 489	29377918	hypothetical protein
22	EF_B0025	25416..27248 [+], 1833	29377919	TraG family protein	PcfC
23	EF_B0026	27298..29457 [+], 2160	29377920	hypothetical protein
24	EF_B0027	29490..29762 [+], 273	29377921	LtrD-related protein, putative
25	EF_B0029	30008..30364 [+], 357	29377922	hypothetical protein
26	EF_B0030	30365..32050 [+], 1686	29377923	relaxase
27	EF_B0031	32104..32433 [+], 330	29377924	hypothetical protein	PcfH
28	EF_B0032	32757..32924 [+], 168	29377925	hypothetical protein
29	EF_B0033	32950..34293 [-], 1344	29377926	hypothetical protein
30	EF_B0034	34284..35060 [-], 777	29377927	hypothetical protein
31	EF_B0035	35203..35751 [-], 549	29377928	hypothetical protein
32	EF_B0036	35775..35948 [-], 174	29377929	hypothetical protein
33	EF_B0037	35951..36136 [-], 186	29377930	hypothetical protein
34	EF_B0038	36142..36591 [-], 450	29377931	hypothetical protein
35	EF_B0040	36878..37078 [+], 201	29377932	hypothetical protein
36	EF_B0041	37179..37409 [-], 231	29377933	hypothetical protein

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	PrgB (29377905)	ND	-	Pheromonedependent overproduction of PrgB induces formation of intercellular aggregates. However, upon overproduction, PrgB confers toxicity on E. faecalis donors by a mechanism dependent on extracellular DNA.	(1) PubMed: 25431047

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Bhatty M et al. (2015). Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence. Mol Microbiol. 95(4):660-77. [PudMed:25431047]

(1) Paulsen IT; Banerjei L; Myers GS; Nelson KE; Seshadri R; Read TD; Fouts DE; Eisen JA; Gill SR; Heidelberg JF; Tettelin H; Dodson RJ; Umayam L; Brinkac L; Beanan M; Daugherty S; DeBoy RT; Durkin S; Kolonay J; Madupu R; Nelson W; Vamathevan J; Tran B; Upton J; Hansen T; Shetty J; Khouri H; Utterback T; Radune D; Ketchum KA; Dougherty BA; Fraser CM (2003). Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 299(5615):2071-4. [PudMed:12663927]