ICEberg
I. Information of IME
ICEberg ID8_IME
Name GIsul2 This is a predicted IME derived from literature
Family-
OrganismPseudomonas aeruginosa FFUP_PS_690
Size (bp)15460 bp
GC content [Genome] (%)
Insertion site3′ end of guaA (GMP synthase)
Functionsul2 (sulphonamide), arsBCHR (arsenate/arsenite)
Species that IME can be transferred to-
Nucleotide SequenceKX709966 (complete IME sequence in this contig)
Replicon-
Coordinates29940..45399
Putative oriT region -
Putative relaxase -


II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid


Detailed Informatioin of the Interaction Network
# IME  Inter_Ele [Type] Methods Donors Recipients Exper_Ref 
1GIsul2 RP4 [IncP plasmid] insolicoin trans - - -

insolico This is a putative interactioin


The graph information of GIsul2 components from KX709966
Complete gene list of GIsul2 from KX709966
#GeneCoordinates [+/-], size (bp) Product *Reannotation 
1-25047..25616 [+], 570hypothetical protein
2-25619..26164 [+], 546hypothetical protein
3-26296..27294 [-], 999hypothetical protein
4dcm128224..29498 [+], 1275Dcm1
5-29506..29943 [+], 438hypothetical protein
6-29982..30158 [+], 177hypothetical protein
7int30149..31450 [+], 1302IntIntegrase 
8alpA31598..31819 [+], 222AlpA
9repA-GIsul231824..32651 [+], 828RepA-GIsul2
10repC-GIsul232782..33519 [+], 738RepC-GIsul2
11stbC34552..34740 [+], 189StbC
12trbJ34809..35660 [+], 852TrbJTrbJ, T4SS component 
13trbK35673..35909 [+], 237TrbK
14trbL35920..37347 [+], 1428TrbLTrbL, T4SS component 
15-37352..37600 [+], 249hypothetical protein
16-37664..37888 [+], 225hypothetical protein
17pemI37925..38128 [-], 204PemI
18arsB38243..39310 [-], 1068ArsB
19arsC39307..39813 [-], 507ArsC
20arsH39810..40577 [-], 768ArsH
21arsR40574..40906 [-], 333ArsR
22resG41054..41791 [+], 738ResG
23rcr242223..43716 [+], 1494Rcr2
24glmS43892..44194 [-], 303GlmS
25sul244281..45096 [-], 816Sul2; sulfonamide insensitive dihydropteroateAR 
26-45403..45714 [+], 312hypothetical protein
27parA45888..46673 [+], 786ParA
28parB46677..47858 [+], 1182ParB
29-48232..48867 [-], 636hypothetical protein
30-49429..49806 [+], 378hypothetical protein
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins20Fasta
(1) Guedon G; Libante V; Coluzzi C; Payot S; Leblond-Bourget N (2017). The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems. Genes (Basel). 8(11). [PudMed:29165361]
(2) Harmer CJ; Hamidian M; Hall RM (2017). pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GIsul2 and a novel class II mercury resistance transposon. Plasmid. 92:17-25. [PudMed:28577759]
(3) Hamidian M; Hall RM (2017). Acinetobacter baumannii ATCC 19606 Carries GIsul2 in a Genomic Island Located in the Chromosome. Antimicrob Agents Chemother. 61(1). [PudMed:27795382]
(4) Nigro SJ; Hall RM (2011). GIsul2, a genomic island carrying the sul2 sulphonamide resistance gene and the small mobile element CR2 found in the Enterobacter cloacae subspecies cloacae type strain ATCC 13047 from 1890, Shigella flexneri ATCC 700930 from 1954 and Acinetobacter baumannii ATCC 17978 from 1951. J Antimicrob Chemother. 66(9):2175-6. [PudMed:21653606]
 

Genomics & Bioinformatics Group in MML, SJTU