I. Information of IME

ICEberg ID	2_IME
Name	BcenGI2
Family	-
Organism	Burkholderia cenocepacia
Size (bp)	16.4 kb
GC content [Genome] (%)
Insertion site	3′ of a tRNAAla gene
Function	blaNDM-1
Species that IME can be transferred to	-
Nucleotide Sequence	AM747720 (complete IME sequence in this genome)
Replicon	-
Genome coordinates	188011..204429
Putative oriT region	-
Putative relaxase	-

II. IME interaction with ICE/CIME/Plasmids

The interaction information of BcenGI2  is not available.

The graph information of BcenGI2  components from AM747720
Complete gene list of BcenGI2  from AM747720
#	Gene	Coordinates [+/-], size (bp)	Product	*Reannotation
1	BCAL0159	183928..184539 [+], 612	putative RlpA-like lipoprotein
2	BCAL0160	184618..185505 [-], 888	putative methylase
3	BCAL0161	185552..185980 [+], 429	conserved hypothetical protein
4	gmhA	186107..186691 [+], 585	phosphoheptose isomerase
5	BCAL0163	186688..187509 [+], 822	putative phospholipid-binding lipoprotein
6	BCAL0164	187506..187868 [+], 363	putative cytochrome c-551 precursor
7	BCAL0165	188900..189877 [+], 978	putative plasmid replication-associated protein
8	BCAL0166	190294..191130 [-], 837	putative transposase	Integrase
9	BCAL0167	191130..191654 [-], 525	putative transposase
10	BCAL0169	192105..192797 [-], 693	conserved hypothetical protein
11	BCAL0170	192918..193169 [+], 252	conserved hypothetical protein
12	BCAL0171	193147..193539 [+], 393	putative plasmid conjugal transfer protein
13	BCAL0172	193882..194244 [+], 363	putative plasmid conjugal transfer protein
14	BCAL0173	194371..195144 [+], 774	putative plasmid conjugal transfer protein	TrbJ, T4SS component
15	BCAL0173a	195154..195345 [+], 192	hypothetical protein
16	BCAL0174	195365..196792 [+], 1428	putative plasmid conjugal transfer protein	TrbL, T4SS component
17	BCAL0175	196857..197147 [+], 291	conserved hypothetical protein
18	BCAL0176	197138..197497 [+], 360	conserved hypothetical protein
19	BCAL0177	197494..197796 [+], 303	putative plasmid conjugal transfer protein
20	BCAL0178	198105..198917 [+], 813	putative DNA methyltransferase
21	BCAL0179	198900..201533 [-], 2634	hypothetical protein
22	BCAL0180	201536..202231 [-], 696	putative membrane protein
23	BCAL0181	202239..202880 [-], 642	conserved hypothetical protein
24	BCAL0182	203211..204338 [-], 1128	putative plasmid recombinase
25	gudD	204567..205964 [-], 1398	putative glucarate dehydratase
26	gudP	206027..207367 [-], 1341	putative glucarate transporter
27	BCAL0185	207582..208505 [-], 924	LysR family regulatory protein
28	BCAL0186	208597..209328 [+], 732	putative glutathione S-transferase

Flanking regions

Gene may contribute to site-specific recombination

Gene may play role in conjugative transfer

(1) Bellanger X; Payot S; Leblond-Bourget N; Guedon G (2014). Conjugative and mobilizable genomic islands in bacteria: evolution and diversity. FEMS Microbiol Rev. 38(4):720-60. [PudMed:24372381]

(2) Graindorge A; Menard A; Monnez C; Cournoyer B (2012). Insertion sequence evolutionary patterns highlight convergent genetic inactivations and recent genomic island acquisitions among epidemic Burkholderia cenocepacia. J Med Microbiol. 61(Pt 3):394-409. [PudMed:21980044]

(3) Holden MT; Seth-Smith HM; Crossman LC; Sebaihia M; Bentley SD; Cerdeno-Tarraga AM; Thomson NR; Bason N; Quail MA; Sharp S; Cherevach I; Churcher C; Goodhead I; Hauser H; Holroyd N; Mungall K; Scott P; Walker D; White B; Rose H; Iversen P; Mil-Homens D; Rocha EP; Fialho AM; Baldwin A; Dowson C; Barrell BG; Govan JR; Vandamme P; Hart CA; Mahenthiralingam E; Parkhill J (2009). The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients. J Bacteriol. 191(1):261-77. [PudMed:18931103]