I. Information of IME
ICEberg ID241_IME
Name Tn4453 This IME is derived from experimental literature
OrganismClostridium difficile
Size (bp)6347 bp
GC content [Genome] (%)
Insertion siteNumerous sites (GA)
FunctioncatD, catP
Species that IME can be transferred to-
Nucleotide SequenceAF226276 (complete IME sequence in this contig)
Putative oriT region -
Putative relaxase coordinates: 5661..6926; Gene: tnpZ;  Family:  MOBV

II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid

Detailed Informatioin of the Interaction Network
# IME  Inter_Ele [Type] Methods Donors Recipients Exper_Ref 
1Tn4453 RP4 [IncP plasmid] insolicoin trans - - -

insolico This is a putative interactioin

The graph information of Tn4453 components from AF226276
Complete gene list of Tn4453 from AF226276
#GeneCoordinates [+/-], size (bp) Product *Reannotation 
1tnpX983..3106 [+], 2124TnpXIntegrase 
2tnpV3031..3402 [+], 372TnpV
3catD3584..4207 [+], 624CatDAR 
4tnpY4457..5479 [+], 1023TnpY
5tnpZ5661..6926 [+], 1266TnpZRelaxase 
6tnpW7050..7238 [+], 189TnpW
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) Bellanger X; Payot S; Leblond-Bourget N; Guedon G (2014). Conjugative and mobilizable genomic islands in bacteria: evolution and diversity. FEMS Microbiol Rev. 38(4):720-60. [PudMed:24372381]
(2) Adams V; Lucet IS; Lyras D; Rood JI (2004). DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 family. Mol Microbiol. 53(4):1195-207. [PudMed:15306021]
(3) Lyras D; Adams V; Lucet I; Rood JI (2004). The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements. Mol Microbiol. 51(6):1787-800. [PudMed:15009902]
(4) Lyras D; Rood JI (2000). Transposition of Tn4451 and Tn4453 involves a circular intermediate that forms a promoter for the large resolvase, TnpX. Mol Microbiol. 38(3):588-601. [PudMed:11069682]
(5) Lyras D; Storie C; Huggins AS; Crellin PK; Bannam TL; Rood JI (1998). Chloramphenicol resistance in Clostridium difficile is encoded on Tn4453 transposons that are closely related to Tn4451 from Clostridium perfringens. Antimicrob Agents Chemother. 42(7):1563-7. [PudMed:9660983]
(6) Crellin PK; Rood JI (1998). Tn4451 from Clostridium perfringens is a mobilizable transposon that encodes the functional Mob protein, TnpZ. Mol Microbiol. 27(3):631-42. [PudMed:9489674]
(7) Crellin PK; Rood JI (1997). The resolvase/invertase domain of the site-specific recombinase TnpX is functional and recognizes a target sequence that resembles the junction of the circular form of the Clostridium perfringens transposon Tn4451. J Bacteriol. 179(16):5148-56. [PudMed:9260958]
(8) Bannam TL; Crellin PK; Rood JI (1995). Molecular genetics of the chloramphenicol-resistance transposon Tn4451 from Clostridium perfringens: the TnpX site-specific recombinase excises a circular transposon molecule. Mol Microbiol. 16(3):535-51. [PudMed:7565113]
(9) Abraham LJ; Rood JI (1988). The Clostridium perfringens chloramphenicol resistance transposon Tn4451 excises precisely in Escherichia coli. Plasmid. 19(2):164-8. [PudMed:2901770]
(10) Abraham LJ; Rood JI (1987). Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringens. J Bacteriol. 169(4):1579-84. [PudMed:2881919]