ICEberg
I. Information of IME
ICEberg ID213_IME
Name SGI1-K This IME is derived from experimental literature
Family-
OrganismProteus mirabilis PmSC17
Size (bp)48754 bp
GC content [Genome] (%)
Insertion site3'-end of the trmE
FunctionaacCA5, aadA7, blaTEM-1b, strAB, sul1, tet(A), mercuric ion resistance
Species that IME can be transferred toEscherichia coli UB1637
Nucleotide SequenceAY463797 (complete IME sequence in this contig)
Replicon-
Coordinates1146..49899
Putative oriT region -
Putative relaxase -


II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid


Detailed Informatioin of the Interaction Network
# IME  Inter_Ele [Type] Methods Donors Recipients Exper_Ref 
1SGI1-K pRMH760 [IncC plasmid] experimentalin trans Salmonella enterica Escherichia coli 27620651

experimental This is an interactioin derived from experimental literature


The graph information of SGI1-K components from AY463797
Complete gene list of SGI1-K from AY463797
#GeneCoordinates [+/-], size (bp) Product *Reannotation 
1trmE447..1163 [+], 717TrmE
2int SGI1367..2584 [+], 1218integrase
3S0022581..2949 [-], 369S002
4rep3307..4260 [-], 954Rep
5S0044247..4537 [-], 291S004
6orfB6349..7221 [-], 873IS1359 transposase; OrfB
7orfA7218..7562 [-], 345IS1359 transposase; OrfA
8traG8067..11471 [-], 3405TraGTraG_F, T4SS component 
9TraH11475..12899 [-], 1425TraHTraH_F, T4SS component 
10S01313143..14000 [+], 858hypothetical protein
11S01413997..14392 [+], 396hypothetical protein
12S01514807..15076 [-], 270hypothetical protein
13S01615154..15339 [-], 186hypothetical protein
14S01715199..15453 [+], 255hypothetical protein
15S01815350..15667 [-], 318hypothetical protein
16S01915925..16221 [-], 297hypothetical protein
17S02016225..17190 [-], 966putative integrase
18S02117283..17618 [+], 336hypothetical protein
19S02217530..17805 [-], 276hypothetical protein
20S02318166..19806 [-], 1641putative helicase
21S02419824..21749 [-], 1926putative exonuclease
22S02521827..24370 [-], 2544putative proteinase subtilisin-like protein
23S02624391..25380 [-], 990putative ATPase
24resG25459..26043 [-], 585ResG
25intI126317..27330 [-], 1014IntI1 integraseIntegrase 
26aacCA527488..27964 [+], 477aminoglycoside (3) acetyltransferase; AacCA5 or AAC(3)-Ie
27aadA728040..28837 [+], 798aminoglycoside (3'')(9) adenylyltransferase; AAD(3'')(9)
28qacEdelta129001..29348 [+], 348QacEdelta1
29sul129342..30181 [+], 840sulfonamide insensitive dihydropteroate synthaseAR 
30tnp610030525..31319 [+], 795tnp6100
31merE32325..32561 [-], 237MerE
32merD32558..32923 [-], 366MerD
33merA32941..34623 [-], 1683MerA
34merC34675..35097 [-], 423MerC
35merP35133..35408 [-], 276MerP
36merT35422..35772 [-], 351MerT
37merR35844..36278 [+], 435MerR
38tetR(A)36484..37134 [-], 651TetR(A)
39tetA(A)37240..38439 [+], 1200TetA(A)AR 
40strB42179..43015 [-], 837StrBAR 
41strA43015..43818 [-], 804StrAAR 
42tnpA113343925..44782 [-], 858TnpA
43tnp2645682..46386 [+], 705Tnp26
44tnpR46625..47182 [+], 558Tn2 resolvase
45blaTEM-1b47364..48224 [+], 861BlaTEM-1b beta lactamaseAR 
46tnp2648685..49389 [-], 705Tnp26
47yidY50100..51287 [+], 1188YidYAR 
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins45Fasta
(1) de Curraize C; Neuwirth C; Bador J; Chapuis A; Amoureux L; Siebor E (2018). Two new Salmonella genomic islands 1 from Proteus mirabilis and description of blaCTX-M-15 on a variant (SGI1-K7). J Antimicrob Chemother. . [PudMed:29659873]
(2) Guedon G; Libante V; Coluzzi C; Payot S; Leblond-Bourget N (2017). The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems. Genes (Basel). 8(11). [PudMed:29165361]
(3) Harmer CJ; Hamidian M; Ambrose SJ; Hall RM (2016). Destabilization of IncA and IncC plasmids by SGI1 and SGI2 type Salmonella genomic islands. Plasmid. 87-88:51-57. [PudMed:27620651]
(4) Hamidian M; Holt KE; Hall RM (2015). The complete sequence of Salmonella genomic island SGI1-K. J Antimicrob Chemother. 70(1):305-6. [PudMed:25193084]
(5) Doublet B; Praud K; Bertrand S; Collard JM; Weill FX; Cloeckaert A (2008). Novel insertion sequence- and transposon-mediated genetic rearrangements in genomic island SGI1 of Salmonella enterica serovar Kentucky. Antimicrob Agents Chemother. 52(10):3745-54. [PudMed:18676889]
(6) Levings RS; Partridge SR; Djordjevic SP; Hall RM (2007). SGI1-K, a variant of the SGI1 genomic island carrying a mercury resistance region, in Salmonella enterica serovar Kentucky. Antimicrob Agents Chemother. 51(1):317-23. [PudMed:17088481]
 

Genomics & Bioinformatics Group in MML, SJTU