 ICEberg ID | 1_IME |
Name  | ATE-1  |
| Family | - |
| Organism | Trueperella pyogenes |
| Size (bp) | 10540 bp |
| GC content [Genome] (%) | |
| Insertion site | 3′ of guaA (GMP synthase) |
| Function | tet(W) (Tetracycline resistance gene) |
| Species that IME can be transferred to | Escherichia coli |
| Nucleotide Sequence | AY049983 (complete IME sequence in this contig) |
| Replicon | - |
| Coordinates | 2137..12676 |
| Putative oriT region | - |
| Putative relaxase | coordinates: 6458..7801; Gene: mob; Family: MOBV |
The Interaction Network among ICE/IME/CIME/plasmid | ||||||
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| Detailed Informatioin of the Interaction Network | ||||||
| # | IME | Inter_Ele [Type] | Methods | Donors | Recipients | Exper_Ref |
| 1 | ATE-1 | RP4 [IncP plasmid] | in trans | E. coli S17-1 | E. coli DH5αMCR | 11959557 |
| This is an interactioin derived from experimental literature |
The graph information of ATE-1 components from AY049983 | |||||
 | |||||
| Complete gene list of ATE-1 from AY049983 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product | *Reannotation | |
| 1 | rluA | 1..562 [+], 562 | putative pseudouridine synthase | ||
| 2 | guaA | 572..2137 [+], 1566 | GMP synthase/glutamine amidotransferase | ||
| 3 | - | 2553..3068 [+], 516 | unknown | ||
| 4 | - | 3192..3398 [+], 207 | HTH protein | ||
| 5 | Int | 3379..4461 [+], 1083 | DNA integrase | Integrase | |
| 6 | nikA | 4465..4785 [+], 321 | putative mobilization potein | ||
| 7 | - | 4793..5947 [+], 1155 | unknown | ||
| 8 | mob | 6458..7801 [+], 1344 | mobilization protein | Relaxase | |
| 9 | - | 8212..8514 [+], 303 | unknown | ||
| 10 | tet(W) | 8309..10228 [+], 1920 | ribosomal protection tetracycline resistance protein | AR | |
| 11 | - | 10773..11105 [-], 333 | unknown | ||
| 12 | - | 11766..12029 [+], 264 | unknown | ||
| 13 | - | 12047..12676 [+], 630 | unknown | ||
| 14 | doc | 12673..12999 [+], 327 | putative death on curing protein | ||
| 15 | pcrA | 13352..15958 [+], 2607 | helicase | ||
 Flanking regions |
 Gene may contribute to site-specific recombination |
 Gene may play role in conjugative transfer |
 Gene may be involved in adaptative function |
| (1) Guedon G; Libante V; Coluzzi C; Payot S; Leblond-Bourget N (2017). The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems. Genes (Basel). 8(11). [PudMed:29165361] |
| (2) Bellanger X; Payot S; Leblond-Bourget N; Guedon G (2014). Conjugative and mobilizable genomic islands in bacteria: evolution and diversity. FEMS Microbiol Rev. 38(4):720-60. [PudMed:24372381] |
| (3) Billington SJ; Songer JG; Jost BH (2002). Widespread distribution of a tet W determinant among tetracycline-resistant isolates of the animal pathogen Arcanobacterium pyogenes. Antimicrob Agents Chemother. 46(5):1281-7. [PudMed:11959557] |