ICEberg
I. Information of IME
ICEberg ID1_IME
Name ATE-1  This IME is derived from experimental literature
Family-
OrganismTrueperella pyogenes
Size (bp)10540 bp
GC content [Genome] (%)
Insertion site3′ of guaA (GMP synthase)
Functiontet(W) (Tetracycline resistance gene)
Species that IME can be transferred toEscherichia coli
Nucleotide SequenceAY049983 (complete IME sequence in this contig)
Replicon-
Coordinates2137..12676
Putative oriT region -
Putative relaxase coordinates: 6458..7801; Gene: mob;  Family:  MOBV


II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid


Detailed Informatioin of the Interaction Network
# IME  Inter_Ele [Type] Methods Donors Recipients Exper_Ref 
1ATE-1  RP4 [IncP plasmid] experimentalin trans E. coli S17-1 E. coli DH5αMCR 11959557

experimental This is an interactioin derived from experimental literature


The graph information of ATE-1  components from AY049983
Complete gene list of ATE-1  from AY049983
#GeneCoordinates [+/-], size (bp) Product *Reannotation 
1rluA1..562 [+], 562putative pseudouridine synthase
2guaA572..2137 [+], 1566GMP synthase/glutamine amidotransferase
3-2553..3068 [+], 516unknown
4-3192..3398 [+], 207HTH protein
5Int3379..4461 [+], 1083DNA integraseIntegrase 
6nikA4465..4785 [+], 321putative mobilization potein
7-4793..5947 [+], 1155unknown
8mob6458..7801 [+], 1344mobilization proteinRelaxase 
9-8212..8514 [+], 303unknown
10tet(W)8309..10228 [+], 1920ribosomal protection tetracycline resistance proteinAR 
11-10773..11105 [-], 333unknown
12-11766..12029 [+], 264unknown
13-12047..12676 [+], 630unknown
14doc12673..12999 [+], 327putative death on curing protein
15pcrA13352..15958 [+], 2607helicase
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins11Fasta
(1) Guedon G; Libante V; Coluzzi C; Payot S; Leblond-Bourget N (2017). The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems. Genes (Basel). 8(11). [PudMed:29165361]
(2) Bellanger X; Payot S; Leblond-Bourget N; Guedon G (2014). Conjugative and mobilizable genomic islands in bacteria: evolution and diversity. FEMS Microbiol Rev. 38(4):720-60. [PudMed:24372381]
(3) Billington SJ; Songer JG; Jost BH (2002). Widespread distribution of a tet W determinant among tetracycline-resistant isolates of the animal pathogen Arcanobacterium pyogenes. Antimicrob Agents Chemother. 46(5):1281-7. [PudMed:11959557]
 

Genomics & Bioinformatics Group in MML, SJTU