I. Information of IME
ICEberg ID199_IME
Name PGI2 This IME is derived from experimental literature
OrganismProteus mirabilis BC11-24
Size (bp)61578 bp
GC content [Genome] (%)
Insertion site3'-end of the trmE
FunctiondfrA16 (trimethoprim), blaPSE-1 (ampicillin), aadA2 (streptomycin and spectinomycin), cmlA1 (chloramphenicol), aadA1 (streptomycin and spectinomycin), aacC4 (apramycin, netilmicin and tobramycin), hph (hygromycin), sul2 (sulphonamides) and floR (chloramphenicol and florfenicol) resistance genes
Species that IME can be transferred toEscherichia coli J53
Nucleotide SequenceMG201402 (complete IME sequence in this contig)
Putative oriT region -
Putative relaxase -

II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid

Detailed Informatioin of the Interaction Network
# IME  Inter_Ele [Type] Methods Donors Recipients Exper_Ref 
1PGI2 pPm14C18 [IncC plasmid] experimentalin trans Proteus mirabilis E. coli J53 29463532

experimental This is an interactioin derived from experimental literature

The graph information of PGI2 components from MG201402
Complete gene list of PGI2 from MG201402
#GeneCoordinates [+/-], size (bp) Product *Reannotation 
1trmE53..1417 [+], 1365tRNA modification GTPase
2int1620..2843 [+], 1224PGI2 integrase
3xis2840..3208 [-], 369PGI2 excisionase
4rep3563..4516 [-], 954PGI2 Rep
5PGI2-0044503..4793 [-], 291hypothetical protein
6PGI2-0054881..7640 [-], 2760hypothetical proteinTraN_F, T4SS component 
7PGI2-0067737..8270 [-], 534hypothetical protein
8PGI2-0078273..8884 [-], 612hypothetical protein
9PGI2-0088884..9105 [-], 222hypothetical protein
10PGI2-0099280..9570 [+], 291hypothetical protein
11PGI2-0109590..9844 [-], 255hypothetical protein
12traG9844..13248 [-], 3405TraGTraG_F, T4SS component 
13traH13252..14676 [-], 1425TraHTraH_F, T4SS component 
14PGI2-01314920..15777 [+], 858hypothetical protein
15PGI2-01415774..16169 [+], 396hypothetical protein
16PGI2-01516584..16853 [-], 270hypothetical protein
17PGI2-01616931..17116 [-], 186hypothetical protein
18PGI2-01716976..17230 [+], 255hypothetical protein
19PGI2-01817127..17444 [-], 318hypothetical protein
20PGI2-01917702..17998 [-], 297hypothetical protein
21PGI2-02018002..18967 [-], 966Int
22PGI2-02119060..19521 [+], 462hypothetical protein
23PGI2-02219307..19627 [-], 321hypothetical protein
24PGI2-02320071..21006 [+], 936hypothetical protein
25PGI2-02421003..21539 [+], 537hypothetical protein
26intI121812..22825 [-], 1014IntI1
27dfrA1622981..23454 [+], 474dihydrofolate reductaseAR 
28blaPSE-123619..24485 [+], 867PSE-1 beta-lactamaseAR 
29aadA224603..25394 [+], 792streptomycin/spectinomycin 3' adenyltransferaseAR 
30cmlA125656..26915 [+], 1260chloramphenicol efflux pumpAR 
31aadA127008..27799 [+], 792aminoglycoside-3'-adenylyltransferaseAR 
32qacEdelta1_127963..28310 [+], 348quaternary ammonium compound resistance protein
33sul1_128304..29143 [+], 840Sul1AR 
34IS26 tnpA_129307..30011 [-], 705IS26 transposase
35PGI2-03430207..30599 [+], 393NimC/NimA family protein
36blms30919..31203 [-], 285Bleomycin resistance proteinAR 
37IS26 tnpA_231499..32203 [-], 705IS26 transposase
38aphA132354..33169 [+], 816aminoglycoside phosphotransferaseAR 
39IS26 tnpA_333359..34063 [-], 705IS26 transposase
40aac(3)-IV34150..34953 [+], 804aminoglycoside acetyltransferaseAR 
41hph35182..36207 [+], 1026hygromycin B phosphotransferase Hph
42PGI2-04136282..36863 [+], 582hypothetical protein
43PGI2-04236629..37381 [-], 753hypothetical protein
44Tn3 tnpA37365..38966 [+], 1602transposase of Tn3
45PGI2-04439192..39677 [+], 486hypothetical protein
46IS4 tnpA39874..40320 [-], 447transposase of IS4
47tnp40395..40964 [-], 570transposase
48sul241054..41869 [+], 816dihydropteroate synthase type IIAR 
49glmM41956..42258 [+], 303phosphoglucosamine mutase GlmM
50rcr242434..43357 [-], 924rolling circle replicase Rcr2
51lysR44039..44344 [-], 306transcriptional regulator
52floR44372..45586 [-], 1215florfenicol/chloramphenicol export protein FloRAR 
53PGI2-05245803..46687 [-], 885hypothetical protein
54PGI2-05346718..47554 [-], 837hypothetical protein
55IS1006 tnpA47612..48316 [+], 705transposase
56Tn21 tnpA48811..51762 [-], 2952transposase of Tn21
57tnpR51765..52325 [-], 561resolvase of Tn21
58tnpM52451..52651 [-], 201TnpM
59intI1-253004..54017 [-], 1014IntI1Integrase 
60aadA2_254163..54954 [+], 792AadA2AR 
61lnuF55086..55907 [+], 822lincosamide nucleotidyltransferase
62qacEdelta1_256055..56402 [+], 348QacEdelta1
63sul1_256396..57235 [+], 840Sul1AR 
64-57363..57863 [+], 501putative acetyltransferase
65-57887..58174 [+], 288hypothetical protein
66IS6100 tnpA58340..59134 [+], 795transposase of IS6100
67PGI2-06659352..59933 [+], 582hypothetical protein
68PGI2-06760194..61066 [-], 873hypothetical protein
69PGI2-06861056..62582 [-], 1527hypothetical protein
70PMI312463404..63868 [+], 465hypothetical protein
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
(1) Lei CW; Chen YP; Kong LH; Zeng JX; Wang YX; Zhang AY; Wang HN (2018). PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis. Antimicrob Agents Chemother. 62(5). [PudMed:29463532]