ICEberg

IME
Gene list
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I. Information of IME

ICEberg ID	199_IME
Name	PGI2
Family	-
Organism	Proteus mirabilis BC11-24
Size (bp)	61578 bp
GC content [Genome] (%)
Insertion site	3'-end of the trmE
Function	dfrA16 (trimethoprim), bla_PSE-1 (ampicillin), aadA2 (streptomycin and spectinomycin), cmlA1 (chloramphenicol), aadA1 (streptomycin and spectinomycin), aacC4 (apramycin, netilmicin and tobramycin), hph (hygromycin), sul2 (sulphonamides) and floR (chloramphenicol and florfenicol) resistance genes
Species that IME can be transferred to	Escherichia coli J53
Nucleotide Sequence	MG201402 (complete IME sequence in this contig)
Replicon	-
Coordinates	1400..62977
Putative oriT region	-
Putative relaxase	-

II. IME interaction with ICE/CIME/Plasmids

The Interaction Network among ICE/IME/CIME/plasmid

Detailed Informatioin of the Interaction Network
#	IME	Inter_Ele [Type]	Methods	Donors	Recipients	Exper_Ref
1	PGI2	pPm14C18 [IncC plasmid]	in trans	Proteus mirabilis	E. coli J53	29463532

This is an interactioin derived from experimental literature

The graph information of PGI2 components from MG201402

Complete gene list of PGI2 from MG201402
#	Gene	Coordinates [+/-], size (bp)	Product	*Reannotation
1	trmE	53..1417 [+], 1365	tRNA modification GTPase
2	int	1620..2843 [+], 1224	PGI2 integrase
3	xis	2840..3208 [-], 369	PGI2 excisionase
4	rep	3563..4516 [-], 954	PGI2 Rep
5	PGI2-004	4503..4793 [-], 291	hypothetical protein
6	PGI2-005	4881..7640 [-], 2760	hypothetical protein	TraN_F, T4SS component
7	PGI2-006	7737..8270 [-], 534	hypothetical protein
8	PGI2-007	8273..8884 [-], 612	hypothetical protein
9	PGI2-008	8884..9105 [-], 222	hypothetical protein
10	PGI2-009	9280..9570 [+], 291	hypothetical protein
11	PGI2-010	9590..9844 [-], 255	hypothetical protein
12	traG	9844..13248 [-], 3405	TraG	TraG_F, T4SS component
13	traH	13252..14676 [-], 1425	TraH	TraH_F, T4SS component
14	PGI2-013	14920..15777 [+], 858	hypothetical protein
15	PGI2-014	15774..16169 [+], 396	hypothetical protein
16	PGI2-015	16584..16853 [-], 270	hypothetical protein
17	PGI2-016	16931..17116 [-], 186	hypothetical protein
18	PGI2-017	16976..17230 [+], 255	hypothetical protein
19	PGI2-018	17127..17444 [-], 318	hypothetical protein
20	PGI2-019	17702..17998 [-], 297	hypothetical protein
21	PGI2-020	18002..18967 [-], 966	Int
22	PGI2-021	19060..19521 [+], 462	hypothetical protein
23	PGI2-022	19307..19627 [-], 321	hypothetical protein
24	PGI2-023	20071..21006 [+], 936	hypothetical protein
25	PGI2-024	21003..21539 [+], 537	hypothetical protein
26	intI1	21812..22825 [-], 1014	IntI1
27	dfrA16	22981..23454 [+], 474	dihydrofolate reductase	AR
28	blaPSE-1	23619..24485 [+], 867	PSE-1 beta-lactamase	AR
29	aadA2	24603..25394 [+], 792	streptomycin/spectinomycin 3' adenyltransferase	AR
30	cmlA1	25656..26915 [+], 1260	chloramphenicol efflux pump	AR
31	aadA1	27008..27799 [+], 792	aminoglycoside-3'-adenylyltransferase	AR
32	qacEdelta1_1	27963..28310 [+], 348	quaternary ammonium compound resistance protein
33	sul1_1	28304..29143 [+], 840	Sul1	AR
34	IS26 tnpA_1	29307..30011 [-], 705	IS26 transposase
35	PGI2-034	30207..30599 [+], 393	NimC/NimA family protein
36	blms	30919..31203 [-], 285	Bleomycin resistance protein	AR
37	IS26 tnpA_2	31499..32203 [-], 705	IS26 transposase
38	aphA1	32354..33169 [+], 816	aminoglycoside phosphotransferase	AR
39	IS26 tnpA_3	33359..34063 [-], 705	IS26 transposase
40	aac(3)-IV	34150..34953 [+], 804	aminoglycoside acetyltransferase	AR
41	hph	35182..36207 [+], 1026	hygromycin B phosphotransferase Hph
42	PGI2-041	36282..36863 [+], 582	hypothetical protein
43	PGI2-042	36629..37381 [-], 753	hypothetical protein
44	Tn3 tnpA	37365..38966 [+], 1602	transposase of Tn3
45	PGI2-044	39192..39677 [+], 486	hypothetical protein
46	IS4 tnpA	39874..40320 [-], 447	transposase of IS4
47	tnp	40395..40964 [-], 570	transposase
48	sul2	41054..41869 [+], 816	dihydropteroate synthase type II	AR
49	glmM	41956..42258 [+], 303	phosphoglucosamine mutase GlmM
50	rcr2	42434..43357 [-], 924	rolling circle replicase Rcr2
51	lysR	44039..44344 [-], 306	transcriptional regulator
52	floR	44372..45586 [-], 1215	florfenicol/chloramphenicol export protein FloR	AR
53	PGI2-052	45803..46687 [-], 885	hypothetical protein
54	PGI2-053	46718..47554 [-], 837	hypothetical protein
55	IS1006 tnpA	47612..48316 [+], 705	transposase
56	Tn21 tnpA	48811..51762 [-], 2952	transposase of Tn21
57	tnpR	51765..52325 [-], 561	resolvase of Tn21
58	tnpM	52451..52651 [-], 201	TnpM
59	intI1-2	53004..54017 [-], 1014	IntI1	Integrase
60	aadA2_2	54163..54954 [+], 792	AadA2	AR
61	lnuF	55086..55907 [+], 822	lincosamide nucleotidyltransferase
62	qacEdelta1_2	56055..56402 [+], 348	QacEdelta1
63	sul1_2	56396..57235 [+], 840	Sul1	AR
64	-	57363..57863 [+], 501	putative acetyltransferase
65	-	57887..58174 [+], 288	hypothetical protein
66	IS6100 tnpA	58340..59134 [+], 795	transposase of IS6100
67	PGI2-066	59352..59933 [+], 582	hypothetical protein
68	PGI2-067	60194..61066 [-], 873	hypothetical protein
69	PGI2-068	61056..62582 [-], 1527	hypothetical protein
70	PMI3124	63404..63868 [+], 465	hypothetical protein

Flanking regions

Gene may contribute to site-specific recombination

Gene may play role in conjugative transfer

Gene may be involved in adaptative function

Element	No. of sequences	Download
Nucleotide sequences	1	Fasta
Proteins	68	Fasta

(1) Lei CW; Chen YP; Kong LH; Zeng JX; Wang YX; Zhang AY; Wang HN (2018). PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis. Antimicrob Agents Chemother. 62(5). [PudMed:29463532]