ICEberg

ICE
Gene list
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References

I. Information of ICE

ICEberg ID	987
Name	ICEMh1
ICEO ID	ICEO_0000728
Organism	Mannheimia haemolytica 42548
Size (bp)	92345
GC content [Genome] (%)	40.18[41.05]
Insertion site	tRNALeu (DR:5'-GATTTTGAATC-3')
Function	Resistance to streptomycin, kanamycin/neomycin (aphA1), tetracycline [tetR-tet(H)], sulphonamides (sul2).
Species that ICE can be transferred to	Pasteurella multocida
Nucleotide Sequence	CP005383 (partial ICE sequence)
Putative oriT region	-
Putative relaxase	coordinates: 2136369..2138348; Locus tag: MHH_c22640; Family: MOB_H

II. ICE interaction with IME/CIME/

The interaction information of ICEMh1 is not available.

The graph information of ICEMh1 components from CP005383

Incomplete gene list of ICEMh1 from CP005383
#	Gene	Coordinates [+/-], size (bp)	Product (GenBank annotation)	*Reannotation
1	MHH_c22510	2125864..2126262 [-], 399	conjugative transfer region lipoprotein
2	MHH_c22520	2126275..2126739 [-], 465	integrating conjugative element protein
3	MHH_c22530	2126825..2127352 [-], 528	hypothetical protein
4	MHH_c22540	2127555..2128094 [+], 540	acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase
5	aphA1	2128248..2129063 [-], 816	aminoglycoside 3'-phosphotransferase protein AphA	AR
6	strB	2129399..2130229 [-], 831	aminoglycoside 6-phosphotransferase protein StrB	AR
7	strA	2130235..2131095 [-], 861	aminoglycoside 3''-phosphotransferase protein StrA	AR
8	sulII	2131207..2132022 [-], 816	dihydropteroate synthase Sul2	AR
9	MHH_c22590	2132216..2132404 [-], 189	putative transcriptional regulator
10	MHH_c22600	2132863..2133705 [+], 843	putative Mhp-like hydrolase
11	MHH_c22630	2135449..2136213 [-], 765	XerD-like tyrosine recombinase-2 family protein	Integrase
12	MHH_c22640	2136369..2138348 [-], 1980	integrating conjugative element relaxase	Relaxase, MOB_H Family
13	MHH_c22650	2138776..2139573 [-], 798	putative HsdM-like type I restriction-modification system methyltransferase subunit
14	MHH_c22660	2139646..2140392 [-], 747	hypothetical protein
15	MHH_c22670	2140532..2141506 [-], 975	antirestriction protein
16	MHH_c22680	2141751..2142158 [-], 408	hypothetical protein
17	MHH_c22690	2142274..2143116 [-], 843	hypothetical protein
18	MHH_c22700	2143194..2143589 [-], 396	hypothetical protein
19	MHH_c22710	2143688..2143933 [-], 246	hypothetical protein
20	MHH_c22720	2144005..2144358 [-], 354	hypothetical protein
21	MHH_c22730	2144732..2145175 [+], 444	DUF1525 domain-containing integrating conjugative element protein	Tfc24, T4SS component
22	MHH_c22740	2145172..2146113 [+], 942	TraU-like integrating conjugative element protein	Tfc23, T4SS component
23	MHH_c22750	2146131..2148143 [+], 2013	integrating conjugative element protein	Tfc22, T4SS component
24	MHH_c22760	2148164..2148592 [+], 429	hypothetical protein	Tfc21, T4SS component
25	MHH_c22770	2148808..2149278 [+], 471	hypothetical protein
26	MHH_c22780	2149299..2149640 [+], 342	hypothetical protein	Tfc20, T4SS component
27	MHH_c22790	2149686..2151158 [-], 1473	TraG-like protein	Tfc19, T4SS component
28	MHH_c22800	2151169..2151486 [-], 318	hypothetical protein	Tfc18, T4SS component
29	MHH_c22810	2151476..2151904 [-], 429	hypothetical protein	Tfc17, T4SS component
30	MHH_c22820	2151904..2154741 [-], 2838	TraC-like conjugative transfer ATPase	Tfc16, T4SS component
31	MHH_c22830	2154744..2155142 [-], 399	conjugative transfer region lipoprotein	Tfc15, T4SS component
32	MHH_c22840	2155155..2156609 [-], 1455	integrating conjugative element protein	Tfc14, T4SS component
33	MHH_c22850	2156621..2157505 [-], 885	TraK-like DUF3438 domain-containing integrating conjugative element protein	Tfc13, T4SS component
34	MHH_c22860	2157505..2158149 [-], 645	DUF2895 domain-containing integrating conjugative element protein	Tfc12, T4SS component
35	MHH_c22870	2158153..2158518 [-], 366	DUF3487 domain-containing conjugative transfer region protein	Tfc11, T4SS component
36	MHH_c22880	2158541..2158930 [-], 390	DUF2976 domain-containing integrating conjugative element protein	Tfc10, T4SS component
37	MHH_c22890	2158945..2159178 [-], 234	hypothetical protein DUF3262
38	MHH_c22900	2159178..2159504 [-], 327	integrative conjugative element protein	Tfc9, T4SS component
39	MHH_c22910	2159691..2160074 [+], 384	hypothetical protein
40	MHH_c22920	2160106..2160801 [-], 696	DUF4400 domain-containing integrating conjugative element membrane protein	Tfc8, T4SS component
41	MHH_c22930	2160798..2161151 [-], 354	hypothetical protein	Tfc7, T4SS component
42	MHH_c22940	2161144..2163345 [-], 2202	TraD-like conjugative coupling factor, SXT/TOL subfamily	Tfc6, T4SS component
43	MHH_c22950	2163345..2163851 [-], 507	DUF2859 domain-containing integrating conjugative element protein	Tfc5, T4SS component
44	MHH_c22960	2163854..2164624 [-], 771	putative lysozyme-like superfamily	Tfc4, T4SS component
45	MHH_c22970	2164603..2165376 [-], 774	integrating conjugative element protein	Tfc3, T4SS component
46	MHH_c22980	2165376..2166005 [-], 630	PilL-like conjugative transfer region protein	Tfc2, T4SS component
47	MHH_c22990	2166164..2166424 [-], 261	hypothetical protein
48	MHH_c23000	2166537..2166899 [-], 363	hypothetical protein
49	MHH_c23010	2166979..2167386 [-], 408	hypothetical protein
50	MHH_c23020	2167412..2168092 [-], 681	hypothetical protein
51	MHH_c23030	2168198..2168629 [-], 432	hypothetical protein DUF3577
52	topB1	2169447..2171498 [-], 2052	DNA topoisomerase 3 TopB
53	MHH_c23050	2171658..2172176 [-], 519	hypothetical protein
54	MHH_c23060	2172273..2172557 [-], 285	hypothetical protein
55	MHH_c23070	2172653..2173363 [+], 711	hypothetical protein
56	MHH_c23080	2173902..2174669 [-], 768	Tas-like aldo/keto reductase
57	MHH_c23090	2174668..2174988 [+], 321	hypothetical protein
58	MHH_c23100	2175170..2175625 [+], 456	putative DUF411 domain-containing metal-binding protein
59	mco	2175638..2177185 [+], 1548	multicopper oxidase Mco
60	MHH_c23120	2177275..2177430 [-], 156	zinc-binding domain-containing alcohol dehydrogenase
61	MHH_c23130	2177519..2177698 [+], 180	hypothetical protein
62	MHH_c23140	2177747..2178265 [+], 519	putative NADH kinase
63	tetR	2178268..2178891 [-], 624	tetracycline repressor protein TetR
64	tetH	2178983..2180185 [+], 1203	tetracycline efflux protein, class H	AR
65	MHH_c23170	2180281..2181591 [-], 1311	ISPst9-like transposase
66	MHH_c23190	2181591..2181893 [-], 303	hypothetical protein
67	MHH_c23200	2181934..2182122 [-], 189	hypothetical protein
68	ssb3	2182429..2182881 [-], 453	single-stranded DNA-binding protein Ssb
69	MHH_c23220	2183237..2183722 [-], 486	hypothetical protein DUF3158
70	MHH_c23230	2183731..2184489 [-], 759	DUF1845 domain-containing integrating conjugative element protein
71	MHH_c23240	2184668..2185858 [-], 1191	hypothetical protein
72	MHH_c23250	2186017..2186574 [-], 558	hypothetical protein DUF2857
73	MHH_c23260	2186567..2188213 [-], 1647	ParB-like integrating conjugative element protein
74	dnaB2	2188222..2189583 [-], 1362	replicative DNA helicase DnaB
75	MHH_c23280	2189595..2190419 [-], 825	chromosome partitioning ATPase
76	xseA	2190914..2192374 [-], 1461	exodeoxyribonuclease VII - large subunit XseA
77	mltA	2192811..2193917 [+], 1107	membrane-bound lytic murein transglycosylase MltA
78	MHH_c23330	2193930..2194379 [+], 450	hypothetical protein

Flanking regions

Element	No. of sequences	Download
Nucleotide sequences	1	Fasta
Proteins	71	Fasta

(1) Eidam C; Poehlein A; Leimbach A; Michael GB; Kadlec K; Liesegang H; Daniel R; Sweeney MT; Murray RW; Watts JL; Schwarz S (2015). Analysis and comparative genomics of ICEMh1, a novel integrative and conjugative element (ICE) of Mannheimia haemolytica. J Antimicrob Chemother. 70(1):93-7. [PubMed:25239467]

(2) Klima CL; Zaheer R; Cook SR; Booker CW; Hendrick S; Alexander TW; McAllister TA (2014). Pathogens of bovine respiratory disease in North American feedlots conferring multidrug resistance via integrative conjugative elements. J Clin Microbiol. 52(2):438-48. [PubMed:24478472]