I. Information of ICE

ICEberg ID	67
Name	Tn6000 (EfcTn1)
ICEO ID	ICEO_0000173
Organism	Enterococcus casseliflavus 664.1H1
Size (bp)	33262
GC content [Genome] (%)	34.69
Insertion site	ribosomal L31 gene
Function	Tetracycline resistance
Species that ICE can be transferred to	Enterococcus faecium
Nucleotide Sequence	FN555436 (complete ICE sequence in this GenBank file)
Replicon	-
Coordinates	1..33262
Putative oriT region	-
Putative relaxase	coordinates: 10212..11195;   Family:  MOBT

II. ICE interaction with IME/CIME/

The interaction information of Tn6000 (EfcTn1) is not available.

The graph information of Tn6000 (EfcTn1) components from FN555436
Complete gene list of Tn6000 (EfcTn1) from FN555436
#	Gene	Coordinates [+/-], size (bp)	Product  (GenBank annotation)	*Reannotation
1	-	643..2055 [+], 1413	hypothetical protein
2	-	2099..2818 [-], 720	hypothetical protein
3	-	3081..4877 [+], 1797	hypothetical protein
4	-	4968..6047 [+], 1080	hypothetical protein
5	-	6269..6676 [+], 408	hypothetical protein
6	-	6824..7138 [+], 315	hypothetical protein
7	-	7154..7519 [+], 366	hypothetical protein
8	-	7523..8899 [+], 1377	hypothetical protein	Orf21_Tn, T4SS component
9	-	8889..9173 [+], 285	hypothetical protein
10	-	9679..9873 [+], 195	hypothetical protein
11	-	10212..11195 [+], 984	hypothetical protein	Relaxase, MOBT Family
12	-	11209..11430 [+], 222	hypothetical protein	Orf19_Tn, T4SS component
13	-	11447..11947 [+], 501	hypothetical protein
14	vap	12081..12890 [+], 810	virulence associated protein
15	hel	12880..16017 [+], 3138	DEAD-DEAH box helicase
16	-	16164..16457 [+], 294	hypothetical protein	Orf17_Tn, T4SS component
17	-	16744..18888 [+], 2145	hypothetical protein	Orf16_Tn, T4SS component
18	-	18848..21136 [+], 2289	hypothetical protein	Orf15_Tn, T4SS component
19	-	21147..22109 [+], 963	hypothetical protein	Orf14_Tn, T4SS component
20	rvt	22822..24651 [+], 1830	reverse transcriptase
21	-	24747..24812 [+], 66	hypothetical protein	Orf14_Tn, T4SS component
22	-	24829..25746 [+], 918	hypothetical protein	Orf13_Tn, T4SS component
23	tet(S)	26590..28515 [+], 1926	Tet(S)	AR
24	-	28639..28770 [+], 132	hypothetical protein
25	-	29284..29643 [-], 360	hypothetical protein
26	-	30122..30622 [+], 501	hypothetical protein
27	-	30693..30818 [+], 126	hypothetical protein
28	xis6000	31604..31879 [+], 276	Xis6000 protein
29	int6000	31979..33211 [+], 1233	Int6000 protein	Integrase

Gene may contribute to site-specific recombination

(1) Brouwer MS; Mullany P; Roberts AP (2010). Characterization of the conjugative transposon Tn6000 from Enterococcus casseliflavus 664.1H1 (formerly Enterococcus faecium 664.1H1). FEMS Microbiol Lett. 309(1):71-6. [PubMed:20528943]

(2) Roberts AP; Davis IJ; Seville L; Villedieu A; Mullany P (2006). Characterization of the ends and target site of a novel tetracycline resistance-encoding conjugative transposon from Enterococcus faecium 664.1H1. J Bacteriol. 188(12):4356-61. [PubMed:16740942]

experimental literature