 ICEberg ID | 59 |
Name  | ICEBs1  |
| ICEO ID | ICEO_0000112 |
| Organism | Bacillus subtilis subsp. subtilis str. 168 |
| Size (bp) | 20510 |
| GC content [Genome] (%) | 35.7[43.51] |
| Insertion site | tRNA-Leu(BSU_tRNA_51) |
| Function | - |
| Species that ICE can be transferred to | Bacillus and Listeria species |
| Nucleotide Sequence | AL009126 (complete ICE sequence in this genome) |
| Replicon | chromosome (4215606 bp, BioProject:76) [NC_000964] |
| Genome coordinates | 529423..549932 |
| Putative oriT region | - |
| Putative relaxase | coordinates: 534773..535831; Locus tag: BSU04870; Family: MOBT |
The graph information of ICEBs1 components from AL009126 | |||||
 | |||||
| Complete gene list of ICEBs1 from AL009126 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product (GenBank annotation) | *Reannotation | |
| 1 | ydcF | 524492..524785 [+], 294 | hypothetical protein | ||
| 2 | ydcG | 524782..525222 [+], 441 | conserved hypothetical protein | ||
| 3 | ydcH | 525206..525649 [+], 444 | putative transcriptional regulator | ||
| 4 | ydcI | 525743..527902 [+], 2160 | putative RNA helicase | ||
| 5 | ydcK | 528129..528581 [+], 453 | conserved hypothetical protein | ||
| 6 | ydcL (int) | 529505..530611 [-], 1107 | putative phage integrase | Integrase | |
| 7 | immA | 530624..531133 [-], 510 | immunity anti-repressor conserved in prophages | ||
| 8 | immR | 531130..531513 [-], 384 | phage element (ICEBs1)transcriptional regulator (Xre family) | ||
| 9 | sacV (xis) | 531787..531981 [+], 195 | transcriptional regulator | ||
| 10 | ydzL | 531978..532238 [+], 261 | conserved hypothetical protein | ||
| 11 | ydcO | 532292..532552 [+], 261 | hypothetical protein; mobile element region | ||
| 12 | ydcP (helP) | 532922..533302 [+], 381 | conserved hypothetical protein; mobile element region | HelP; ICEBs1-encoded helicase processivity factor; [PMID: 23326247] | |
| 13 | ydcQ (conQ) | 533338..534780 [+], 1443 | putative DNA wielding protein; mobile element region | YdcQ, T4SS component | |
| 14 | ydcR (nicK) | 534773..535831 [+], 1059 | putative replication protein; mobile element region | Relaxase, MOBT Family | |
| 15 | ydcS | 536096..536365 [+], 270 | conserved hypothetical protein; mobile element region | ||
| 16 | ydcT | 536404..536670 [+], 267 | conserved hypothetical protein; mobile element region | ||
| 17 | yddA | 536687..536995 [+], 309 | conserved hypothetical protein; mobile element region | ||
| 18 | yddB (conB) | 536985..538049 [+], 1065 | conserved hypothetical protein; mobile element region | YddB, T4SS component | |
| 19 | yddC (conC) | 538061..538309 [+], 249 | conserved hypothetical protein | YddC, T4SS component | |
| 20 | yddD (conD) | 538322..538846 [+], 525 | hypothetical protein; mobile element region | YddD, T4SS component | |
| 21 | yddE (conE) | 538836..541229 [+], 2394 | conserved hypothetical protein | YddE, T4SS component | |
| 22 | yddF | 541248..541574 [+], 327 | conserved hypothetical protein; mobile element region | ||
| 23 | yddG (conG) | 541578..544025 [+], 2448 | conserved hypothetical protein; mobile element region | YddG, T4SS component | |
| 24 | yddH (cwlT) | 544022..545011 [+], 990 | putative cell wall hydrolase; mobile element region | YddH, T4SS component | |
| 25 | yddI | 545026..545532 [+], 507 | conserved hypothetical protein; mobile element region | ||
| 26 | yddJ | 545595..545975 [+], 381 | putative lipoprotein | ||
| 27 | yddK | 546166..546966 [-], 801 | conserved hypothetical protein; mobile element region | ||
| 28 | rapI | 547306..548481 [+], 1176 | response regulator aspartate phosphatase; mobile element region | ||
| 29 | phrI | 548438..548557 [+], 120 | secreted regulator of the activity of phosphatase RapI | ||
| 30 | yddM | 548710..549651 [+], 942 | putative helicase; mobile element region | ||
| 31 | yddN | 550240..551259 [-], 1020 | putative alkanal monooxygenase | ||
| 32 | lrpA | 551519..551929 [+], 411 | transcriptional regulator (Lrp/AsnC family) | ||
| 33 | lrpB | 552052..552501 [-], 450 | transcriptional regulator (Lrp/AsnC family) | ||
| 34 | yddQ | 552616..553158 [+], 543 | putative hydrolase | ||
| 35 | yddR | 553711..554475 [+], 765 | putative metal-dependent hydrolase | ||
 Flanking regions |
|
(1) Lee CA; Thomas J; Grossman AD (2012). The Bacillus subtilis conjugative transposon ICEBs1 mobilizes plasmids lacking dedicated mobilization functions. J Bacteriol. 194(12):3165-72. [PubMed:22505685]
|
|
(2) Babic A; Berkmen MB; Lee CA; Grossman AD (2011). Efficient gene transfer in bacterial cell chains. MBio. 2(2). [PubMed:21406598]
|
|
(3) Smits WK; Grossman AD (2010). The transcriptional regulator Rok binds A+T-rich DNA and is involved in repression of a mobile genetic element in Bacillus subtilis. PLoS Genet. 6(11):e1001207. [PubMed:21085634]
|
|
(4) Bose B; Grossman AD (2011). Regulation of horizontal gene transfer in Bacillus subtilis by activation of a conserved site-specific protease. J Bacteriol. 193(1):22-9. [PubMed:21036995]
|
|
(5) Lee CA; Babic A; Grossman AD (2010). Autonomous plasmid-like replication of a conjugative transposon. Mol Microbiol. 75(2):268-79. [PubMed:19943900]
|
|
(6) Berkmen MB; Lee CA; Loveday EK; Grossman AD (2010). Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 192(1):38-45. [PubMed:19734305]
|
|
(7) Bose B; Auchtung JM; Lee CA; Grossman AD (2008). A conserved anti-repressor controls horizontal gene transfer by proteolysis. Mol Microbiol. 70(3):570-82. [PubMed:18761623]
|
|
(8) Lee CA; Auchtung JM; Monson RE; Grossman AD (2007). Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis. Mol Microbiol. 66(6):1356-69. [PubMed:18005101]
|
|
(9) Lee CA; Grossman AD (2007). Identification of the origin of transfer (oriT) and DNA relaxase required for conjugation of the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 189(20):7254-61. [PubMed:17693500]
|
|
(10) Goranov AI; Kuester-Schoeck E; Wang JD; Grossman AD (2006). Characterization of the global transcriptional responses to different types of DNA damage and disruption of replication in Bacillus subtilis. J Bacteriol. 188(15):5595-605. [PubMed:16855250]
|
|
(11) Auchtung JM; Lee CA; Monson RE; Lehman AP; Grossman AD (2005). Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response. Proc Natl Acad Sci U S A. 102(35):12554-9. [PubMed:16105942]
|
| experimental literature |