I. Information of ICE

ICEberg ID	542
Name	pCM32 {AICE}
ICEO ID	ICEO_0000737
Organism	Saccharopolyspora endophytica YIM
Size (bp)	14,611
GC content [Genome] (%)	67.2
Insertion site	tRNA(Ser)
Function	improve production of spinosyn in Saccharopolyspora spinosa
Species that ICE can be transferred to	Saccharopolyspora spinosa
Nucleotide Sequence	KM260754 (complete ICE sequence in this GenBank file)
Replicon	-
Coordinates	1..14611
Putative oriT region	-
Putative relaxase	-

II. ICE interaction with IME/CIME/

The interaction information of pCM32 {AICE} is not available.

The graph information of pCM32 {AICE} components from KM260754
Complete gene list of pCM32 {AICE} from KM260754
#	Gene	Coordinates [+/-], size (bp)	Product  (GenBank annotation)	*Reannotation
1	pCM32.1c	9..1238 [-], 1230	hypothetical protein
2	pCM32.2	1271..1633 [+], 363	Rep3
3	pCM23.3c	1639..2004 [-], 366	GntR family transcriptional regulator
4	pCM32.4	2102..2410 [+], 309	transcriptional regulator
5	pCM32.5	2413..2718 [+], 306	WhiB-family transcriptional regulator
6	pCM32.6c	2703..2939 [-], 237	hypothetical protein
7	pCM32.7	3083..3490 [+], 408	hypothetical protein
8	pCM32.8	3497..4447 [+], 951	TraB
9	pCM32.9	4466..4927 [+], 462	hypothetical protein
10	pCM32.10c	4914..5141 [-], 228	hypothetical protein
11	pCM32.11	5207..5485 [+], 279	hypothetical protein
12	pCM32.12	5482..6780 [+], 1299	hypothetical protein
13	pCM32.13	6895..9096 [+], 2202	cell division protein FtsK/SpoIIIE
14	pCM32.14	9295..10308 [+], 1014	Rep1
15	pCM32.15	10305..11903 [+], 1599	Rep2
16	pCM32.16	12287..12493 [+], 207	Excisionase/Xis
17	pCM32.17	12549..14036 [+], 1488	Integrase	Integrase

Gene may contribute to site-specific recombination

(1) Chen J; Xia H; Dang F; Xu Q; Li W; Qin Z (2015). Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Appl Microbiol Biotechnol. 99(23):10141-9. [PubMed:26260388]

experimental literature

in silico analysis literature