ICEberg

ICE
Gene list
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I. Information of ICE

ICEberg ID	46
Name	Tn4371
ICEO ID	ICEO_0000097
Organism	Ralstonia oxalatica A5
Size (bp)	54657
GC content [Genome] (%)	63.49
Insertion site	TTTTTCAT
Function	Degrade biphenyl and 4-chlorobiphenyl
Species that ICE can be transferred to	Cupriavidus metallidurans
Nucleotide Sequence	AJ536756 (complete ICE sequence in this GenBank file)
Replicon	-
Coordinates	1..54657
Putative oriT region	-
Putative relaxase	coordinates: 26607..28160; Family: MOB_L

II. ICE interaction with IME/CIME/

The interaction information of Tn4371 is not available.

The graph information of Tn4371 components from AJ536756

Complete gene list of Tn4371 from AJ536756
#	Gene	Coordinates [+/-], size (bp)	Product (GenBank annotation)	*Reannotation
1	int	181..1389 [+], 1209	putative site specific recombinase	Integrase
2	-	1469..1765 [+], 297	hypothetical protein
3	-	1762..2169 [+], 408	hypothetical protein
4	-	2210..3385 [-], 1176	hypothetical protein
5	-	3388..4533 [+], 1146	putative transposase
6	-	4813..5835 [-], 1023	hypothetical protein
7	-	5989..7980 [-], 1992	hypothetical protein
8	-	8072..9871 [-], 1800	hypothetical protein
9	-	9874..12012 [-], 2139	hypothetical protein
10	-	12442..12792 [+], 351	hypothetical protein
11	-	13054..13410 [-], 357	hypothetical protein
12	-	13502..13882 [-], 381	hypothetical protein
13	-	14290..15117 [+], 828	hypothetical protein
14	-	15194..17266 [+], 2073	hypothetical protein
15	-	17330..17539 [+], 210	hypothetical protein
16	-	18015..18785 [-], 771	hypothetical protein
17	-	18782..19276 [-], 495	putative transcription regulator
18	-	19931..20329 [+], 399	hypothetical protein	TraF, T4SS component
19	-	20697..20990 [-], 294	putative transcription regulator
20	-	21160..21561 [+], 402	hypothetical protein
21	-	21903..22664 [+], 762	hypothetical protein
22	-	22748..23038 [+], 291	hypothetical protein
23	repA	23065..23922 [+], 858	putative replication initiation protein
24	parA	24188..24826 [+], 639	putative plasmid partition protein
25	parB	24823..25086 [+], 264	hypothetical protein
26	-	25083..25616 [+], 534	hypothetical protein
27	traF	25613..26212 [+], 600	putative plasmid conjugal transfer transmembrane protein	TraF, T4SS component
28	-	26607..28160 [+], 1554	hypothetical protein	Relaxase, MOB_L Family
29	bphS	28524..29468 [-], 945	transcription regulator
30	bphE	29962..30768 [+], 807	2-oxopent-4-enoate hydratase
31	bphG	30771..31712 [+], 942	acetaldehyde dehydrogenase oxidoreductase
32	bphF	31724..32779 [+], 1056	putative 4-hydroxy-2-oxovalerate aldolase transmembrane protein
33	bphA1	33268..34644 [+], 1377	biphenyl 2,3-dioxygenase oxidoreductase
34	bphA2	34676..35257 [+], 582	biphenyl 2,3-dioxygenase
35	bphA3	35275..35604 [+], 330	3-phenylpropionate dioxygenase ferredoxin oxidoreductase
36	bphB	35623..36453 [+], 831	2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase
37	bphC	36467..37348 [+], 882	2,3-dihydroxybiphenyl dioxygenase
38	bphD	37380..38240 [+], 861	2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase
39	-	38365..39021 [+], 657	hypothetical protein
40	bphA4	39116..40411 [+], 1296	3-phenylpropionate dioxygenase ferredoxin oxidoreductase
41	traR	40563..41606 [+], 1044	transcription regulator
42	traG	42118..44121 [+], 2004	putative conjugal transfer protein	VirD4, T4SS component
43	-	44118..44582 [+], 465	hypothetical protein
44	trbB	44579..45616 [+], 1038	putative mating pair formation protein	TrbB, T4SS component
45	trbC	45622..46005 [+], 384	putative mating pair formation transmembrane protein	TrbC, T4SS component
46	trbE	46287..48740 [+], 2454	putative mating pair formation protein	TrbE, T4SS component
47	trbJ	48737..49492 [+], 756	putative mating pair formation protein	TrbJ, T4SS component
48	-	49504..49803 [+], 300	hypothetical protein	TrbJ, T4SS component
49	trbL	49800..51149 [+], 1350	putative mating pair formation transmembrane protein	TrbL, T4SS component
50	trbF	51168..51872 [+], 705	putative mating pair formation protein	TrbF, T4SS component
51	trbG	51869..52849 [+], 981	putative mating pair formation protein	TrbG, T4SS component
52	trbI	52852..54132 [+], 1281	putative mating pair formation transmembrane protein	TrbI, T4SS component
53	-	54129..54374 [+], 246	hypothetical protein

Element	No. of sequences	Download
Nucleotide sequences	1	Fasta
Proteins	53	Fasta

(1) Toussaint A; Merlin C; Monchy S; Benotmane MA; Leplae R; Mergeay M; Springael D (2003). The biphenyl- and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids. Appl Environ Microbiol. 69(8):4837-45. [PubMed:12902278]

(2) Merlin C; Springael D; Toussaint A (1999). Tn4371: A modular structure encoding a phage-like integrase, a Pseudomonas-like catabolic pathway, and RP4/Ti-like transfer functions. Plasmid. 41(1):40-54. [PubMed:9887305]

(3) Springael D; Kreps S; Mergeay M (1993). Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutrophus A5. J Bacteriol. 175(6):1674-81. [PubMed:8383664]

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