ICEberg

ICE
Gene list
Download
References

I. Information of ICE

ICEberg ID	440
Name	ICEPmu1
ICEO ID	ICEO_0000181
Organism	Pasteurella multocida 36950
Size (bp)	82214
GC content [Genome] (%)	41.9
Insertion site	tRNA-Leu [DRs: GATTTTGAATCAA]
Function	Harbored 11 resistance genes, which confer resistance to streptomycin/spectinomycin (aadA25), streptomycin (strA and strB), gentamicin (aadB), kanamycin/neomycin (aphA1), tetracycline [tetR-tet (H)], chloramphenicol/florfenicol (floR), sulphonamides (sul2), tilmicosin/clindamycin [erm (42)] or tilmicosin/tulathromycin [msr (E)- mph (E)]
Species that ICE can be transferred to	Escherichia coli HK22511; Pasteurella multocida E348-08 (capsular type F); Mannheimia haemolytica 39229
Nucleotide Sequence	CP003022 (complete ICE sequence in this genome)
Replicon	[NC_016808]
Genome coordinates	273284..355497
Putative oriT region	-
Putative relaxase	coordinates: 293488..295467; Locus tag: Pmu_02890; Family: MOB_H

II. ICE interaction with IME/CIME/

The interaction information of ICEPmu1 is not available.

The graph information of ICEPmu1 components from CP003022

Complete gene list of ICEPmu1 from CP003022
#	Gene	Coordinates [+/-], size (bp)	Product (GenBank annotation)	*Reannotation
1	Pmu_02660	272295..272966 [-], 672	putative lipoprotein
2	Pmu_02680	273706..274284 [+], 579	hypothetical protein
3	Pmu_02690	275016..275453 [-], 438	putative phage transposase
4	Pmu_02700	275603..276502 [+], 900	tyrosine recombinase-1 family protein	Integrase
5	Pmu_02710	276775..277698 [-], 924	ISApl1 transposase
6	aphA1	279777..280592 [-], 816	aminoglycoside 3'-phosphotransferase protein
7	strB	280928..281764 [-], 837	aminoglycoside 6-phosphotransferase protein
8	strA	281764..282522 [-], 759	aminoglycoside 3''-phosphotransferase protein
9	sul2	282735..283439 [-], 705	dihydropteroate synthase
10	Pmu_02790	283743..285035 [+], 1293	ISCR21 transposase
11	floR	286824..288038 [+], 1215	florfenicol / chloramphenicol efflux protein
12	Pmu_02830	288066..288371 [+], 306	putative LysR transcriptional regulator, HTH domain profile
13	Pmu_02840	288483..289976 [+], 1494	ISCR2 transposase
14	erm(42)	290396..291301 [+], 906	rRNA (adenine-N6-)-methyltransferase
15	Pmu_02880	292568..293332 [-], 765	tyrosine recombinase-2 family protein
16	Pmu_02890	293488..295467 [-], 1980	integrating conjugative element relaxase, PFGI-1 class	Relaxase, MOB_H Family
17	Pmu_02900	295895..296692 [-], 798	putative type I restriction-modification system methyltransferase subunit
18	Pmu_02910	296765..297511 [-], 747	hypothetical protein
19	Pmu_02920	297651..298625 [-], 975	hypothetical protein
20	Pmu_02930	298870..299277 [-], 408	hypothetical protein
21	Pmu_02940	299393..300235 [-], 843	hypothetical protein
22	Pmu_02950	300313..300708 [-], 396	hypothetical protein
23	Pmu_02960	300807..301052 [-], 246	hypothetical protein
24	Pmu_02970	301124..301477 [-], 354	hypothetical protein
25	Pmu_02980	301851..302294 [+], 444	integrating conjugative element protein, PFL family	Tfc24, T4SS component
26	Pmu_02990	302291..303232 [+], 942	integrating conjugative element protein, PFL family	Tfc23, T4SS component
27	Pmu_03000	303250..305262 [+], 2013	integrating conjugative element protein, PFL family	Tfc22, T4SS component
28	Pmu_03010	305283..305711 [+], 429	hypothetical protein	Tfc21, T4SS component
29	Pmu_03020	305927..306397 [+], 471	hypothetical protein
30	Pmu_03030	306418..306759 [+], 342	hypothetical protein	Tfc20, T4SS component
31	Pmu_03040	306805..308277 [-], 1473	TraG-like domain-containing protein	Tfc19, T4SS component
32	Pmu_03050	308288..308605 [-], 318	hypothetical protein	Tfc18, T4SS component
33	Pmu_03060	308595..309023 [-], 429	hypothetical protein	Tfc17, T4SS component
34	Pmu_03070	309023..311860 [-], 2838	conjugative transfer ATPase TraC-like, PFL family	Tfc16, T4SS component
35	Pmu_03080	311863..312261 [-], 399	conjugative transfer region lipoprotein	Tfc15, T4SS component
36	Pmu_03090	312274..313728 [-], 1455	integrating conjugative element protein, PFL family	Tfc14, T4SS component
37	Pmu_03100	313740..314624 [-], 885	integrating conjugative element protein, PFL family	Tfc13, T4SS component
38	Pmu_03110	314624..315268 [-], 645	integrating conjugative element protein, PFL family	Tfc12, T4SS component
39	Pmu_03120	315272..315637 [-], 366	conjugative transfer region protein	Tfc11, T4SS component
40	Pmu_03130	315660..316049 [-], 390	integrating conjugative element protein, PFL family	Tfc10, T4SS component
41	Pmu_03140	316064..316297 [-], 234	hypothetical protein
42	Pmu_03150	316297..316623 [-], 327	integrative conjugative element protein, RAQPRD family	Tfc9, T4SS component
43	Pmu_03160	316810..317193 [+], 384	hypothetical protein
44	Pmu_03170	317225..317920 [-], 696	integrating conjugative element membrane protein, PFL family	Tfc8, T4SS component
45	Pmu_03180	317917..318270 [-], 354	hypothetical protein	Tfc7, T4SS component
46	Pmu_03190	318263..320464 [-], 2202	conjugative coupling factor TraD, SXT/TOL subfamily	Tfc6, T4SS component
47	Pmu_03200	320464..320970 [-], 507	integrating conjugative element protein, PFL family	Tfc5, T4SS component
48	Pmu_03210	320973..321743 [-], 771	putative lysozyme-like superfamily	Tfc4, T4SS component
49	Pmu_03220	321722..322495 [-], 774	integrating conjugative element protein, PFL family	Tfc3, T4SS component
50	Pmu_03230	322495..323124 [-], 630	putative integrating conjugative element protein PilL, PFGI-1 class	Tfc2, T4SS component
51	Pmu_03240	323283..323543 [-], 261	hypothetical protein
52	Pmu_03250	323656..324018 [-], 363	hypothetical protein
53	Pmu_03260	324098..324505 [-], 408	hypothetical protein
54	Pmu_03270	324531..325211 [-], 681	hypothetical protein
55	Pmu_03280	325317..325748 [-], 432	hypothetical protein
56	topB1	326566..328617 [-], 2052	DNA topoisomerase III
57	Pmu_03300	328777..329295 [-], 519	hypothetical protein
58	Pmu_03310	329392..329676 [-], 285	hypothetical protein
59	Pmu_03320	329772..330482 [+], 711	hypothetical protein
60	Pmu_03330	331021..331788 [-], 768	aldo/keto reductase
61	Pmu_03340	331787..332107 [+], 321	hypothetical protein
62	Pmu_03350	332289..332744 [+], 456	hypothetical protein
63	Pmu_03360	332758..334305 [+], 1548	multicopper oxidase protein
64	Pmu_03370	334395..334550 [-], 156	alcohol dehydrogenase, zinc-binding domain protein
65	Pmu_03380	334639..334818 [+], 180	hypothetical protein
66	Pmu_03390	334867..335385 [+], 519	hypothetical protein
67	tetR1	335388..336011 [-], 624	tetracycline repressor protein
68	aadB	336102..336731 [+], 630	aminoglycoside 2''-O-adenyltransferase protein
69	aadA25	336789..337580 [+], 792	aminoglycoside 3''-O-adenyltransferase protein
70	blaOXA-2	337657..338484 [+], 828	beta-lactamase OXA-2 protein
71	Pmu_03450	338575..339279 [+], 705	IS26 transposase
72	msr(E)	339642..341117 [+], 1476	macrolide efflux protein
73	mph(E)	341173..342057 [+], 885	macrolide 2'-phosphotransferase protein
74	Pmu_03480	342141..342845 [+], 705	IS26 transposase
75	tetR2	343099..343722 [-], 624	tetracycline repressor protein
76	tet(H)	343814..345016 [+], 1203	tetracycline efflux protein, class H
77	Pmu_03510	345112..346422 [-], 1311	transposase tnpA
78	Pmu_03520	346422..346724 [-], 303	hypothetical protein
79	Pmu_03530	346765..346953 [-], 189	hypothetical protein
80	ssb1	347260..347712 [-], 453	single-stranded DNA-binding protein
81	Pmu_03550	348068..348553 [-], 486	hypothetical protein
82	Pmu_03560	348562..349320 [-], 759	integrating conjugative element protein, PFL family
83	Pmu_03570	349499..350689 [-], 1191	hypothetical protein
84	Pmu_03580	350848..351405 [-], 558	hypothetical protein
85	Pmu_03590	351398..353044 [-], 1647	integrating conjugative element ParB family protein, PFGI-1 class
86	dnaB1	353053..354339 [-], 1287	replicative DNA helicase
87	Pmu_03610	354426..355250 [-], 825	chromosome partitioning ATPase
88	Pmu_03640	355836..356327 [+], 492	heme iron utilization protein-like protein
89	Pmu_03650	356342..356857 [+], 516	heme iron utilization protein, pyridoxamine 5'-phosphate domain-type
90	hgbA	356899..359802 [+], 2904	hemoglobin-binding protein A
91	rpsO	359969..360238 [+], 270	30S ribosomal protein S15

Flanking regions

Element	No. of sequences	Download
Nucleotide sequences	1	Fasta
Proteins	86	Fasta

(1) Michael GB; Kadlec K; Sweeney MT; Brzuszkiewicz E; Liesegang H; Daniel R; Murray RW; Watts JL; Schwarz S (2012). ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer. J Antimicrob Chemother. 67(1):91-100. [PubMed:22001176]

(2) Michael GB; Kadlec K; Sweeney MT; Brzuszkiewicz E; Liesegang H; Daniel R; Murray RW; Watts JL; Schwarz S (2012). ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes. J Antimicrob Chemother. 67(1):84-90. [PubMed:22001175]

experimental literature